EFFECT OF OVINE GONADOTROPHINS AND ANTISERUM TO INTERSTITIAL CELL-STIMULATING HORMONE ON THE TESTIS OF THE HYPOPHYSECTOMIZED RAT

1963 ◽  
Vol 44 (4) ◽  
pp. 536-544 ◽  
Author(s):  
Ardis J. Lostroh ◽  
Ruth Johnson ◽  
C. W. Jordan
Keyword(s):  
Mitotic Activity ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Interstitial Cell ◽  
Rabbit Antiserum ◽  
Interstitial Tissue ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Stimulating Hormone

ABSTRACT Rabbit antiserum to ovine ICSH was administered to male Sprague-Dawley rats 2 months after hypophysectomy; further atrophy of the testis ensued. The interstitial tissue, the Sertoli cells, and the germ cells were adversely affected by the treatment. In the absence of gonadotrophins and exogenous androgen, spermatogenesis was nearly arrested. Ovine ICSH repaired the interstitial tissue and stimulated mitotic activity among the spermatogonia. Ovine FSH (NIH-FSH-S-1), containing approximately 1% ICSH activity, stimulated spermatid formation. When antiserum to ICSH was injected together with the FSH, no maturation divisions or spermatids were observed. The combination of FSH and ICSH, 100 μg of each per day injected for a period of 21 days, stimulated extensive and uniform tubular repair and advanced spermiogenesis to the acrosome phase; an enhancement in the secretion of androgen also was effected when FSH was given in combination with ICSH.

CHANGES IN SERUM LEVELS OF FOLLICLE STIMULATING HORMONE AND LUTEINIZING HORMONE SHORTLY BEFORE AND AFTER PARTURITION IN RATS

1974 ◽  
Vol 75 (3) ◽  
pp. 491-496 ◽  
Author(s):  
Junichi Mori ◽  
Hiroshi Nagasawa ◽  
Reiko Yanai ◽  
Junji Masaki
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Serum Levels ◽  
Sprague Dawley ◽  
Appreciable Change ◽  
Serum Lh ◽  
Sprague Dawley Rats ◽  
Before And After ◽  
Average Serum ◽  
Stimulating Hormone

ABSTRACT The sequence of changes in the serum levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from 2 days before to 24 h after parturition of primiparous Sprague-Dawley rats was investigated by radioimmunoassay. No appreciable change in average serum FSH levels was observed during 2 days before and 1 h after parturition. After this the levels increased gradually to show a peak at 7 h after parturition and then declined gradually until 24 h after parturition. However, the level at 24 h after parturition was still twice as high as that at parturition (0 h). The average serum LH levels which were low between 2 days before and 1 h after parturition, showed a peak at 7 h and decreased toward 13 h after parturition. The same levels as at parturition were maintained between 13 and 24 h after parturition. The time of surge of either FSH or LH was closely related to the time after parturition. There were some differences between FSH and LH in the patterns of sequence of changes in the serum levels near parturition.

Mesonephric contribution to testis differentiation in the fetal mouse

Development ◽  
1993 ◽  
Vol 117 (1) ◽  
pp. 273-281 ◽  
Author(s):  
M. Buehr ◽  
S. Gu ◽  
A. McLaren
Keyword(s):  
Germ Cells ◽  
Sertoli Cells ◽  
Interstitial Cell ◽  
Mouse Embryos ◽  
Myoid Cells ◽  
Testis Differentiation ◽  
Embryonic Limb ◽  
Female Donor ◽  
Well Differentiated ◽  
Donor Embryo

Testes from 11.5-day-old mouse embryos, with and without attached mesonephroi, were cultured for 7 days. Isolated testes failed to develop well-differentiated testis cords: however, when cultured attached to a mesonephros from either a male or a female donor embryo, testes developed cords that were normal in appearance. Testes cultured next to a mesonephric region but separated from it by a permeable filter, did not develop normal cords, nor did testes grafted to fragments of embryonic limb or heart. When testes were grafted to mesonephric regions from mice carrying a transgenic marker, the marker was found in some of the peritubular myoid cells and other interstitial cells of the testis, but not in the Sertoli cells or the germ cells. We conclude that after 11.5 days post coitum, cells can migrate from the mesonephric region into the differentiating testis and can contribute to the interstitial cell population, and that this contribution is necessary for the establishment of normal cord structure. The germ cells in all cultured testes, whether or not differentiated cords were present, were T1 prospermatogonia: no meiotic germ cells were seen.

Histochemical localization of enzymes of various metabolic pathways in the testes of buffaloes, goats and rams

1984 ◽  
Vol 102 (2) ◽  
pp. 269-274 ◽  
Author(s):  
G. S. Bilaspuri ◽  
S. S. Guraya
Keyword(s):  
Germ Cells ◽  
Sertoli Cells ◽  
Metabolic Pathways ◽  
Maximum Activity ◽  
The Other ◽  
Seminiferous Tubules ◽  
Interstitial Tissue ◽  
Cell Type ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Enzyme Species

SummaryIsocitrate dehydrogenase (ICDH), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), glutamate dehydrogenase (GDH), β-hydroxybutyrate dehydrogenase (β-OH-BDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) were histochemically located in the testes of buffaloes, goats and rams. The enzyme activities varied with the enzyme, species and cell type. The activities in the seminiferous tubules were correlated with the stages of seminiferous epithelial cycle (SEC). During this cycle, the activities in the Sertoli cells, spermatogonia and spermatocytes remained unaltered in contrast to those in the spermatids. The activities of SDH, ICDH and MDH were relatively greater in buffalo, while goat and ram resembled each other quite closely. ICDH and MDH preferred NADP to NAD. In the three species, the activities of ICDH, SDH and MDH generally followed an increasing order. G-6-PDH was greater in the interstitial tissue of buffalo than in goat and ram; the maximum activity of this enzyme in each species was found in the spermatogonia. In comparison with G-6-PDH, GDH was less evident in the interstitial tissue of buffalo and goat; Sertoli cells and spermatogonia also showed relatively less MDH activity whereas the other germ cells may have relatively less, similar or more, GDH activity depending on the species. β-OHBDH activity was similar in the interstitial tissue of the three species, but in the seminiferous tubule, the activity was less in goat. But for GDH and β-OH-BDH which could show different results, the activities of other enzymes generally decreased from spermatogonia through spermatocytes to spermatids but increased during spermiogenesis. In spermatozoa, the enzymes were observed only in the mid-piece. The possible physiological significance of the results is discussed in relation to different metabolic pathways.

scholarly journals Differential expression of glucose transporter GLUT8 during mouse spermatogenesis

Reproduction ◽  
2006 ◽  
Vol 131 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Olga Gómez ◽  
Amparo Romero ◽  
José Terrado ◽  
José E Mesonero
Keyword(s):  
Western Blot ◽  
Differential Expression ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Glucose Transporter ◽  
Mouse Testis ◽  
Pivotal Role ◽  
Interstitial Tissue ◽  
Rt Pcr ◽  
Fuel Supply

GLUT8 is a facilitative glucose transporter expressed at high levels in the testis. In this study, we analyzed the GLUT8 expression in mouse testis during spermatogenesis by RT–PCR, Western blot and immunohistochemistry methods. Our results show that GLUT8 expression is limited to spermatids and spermatozoa in the testis. Expression begins when round spermatids are formed at postnatal day 24. The expression persists throughout spermiogenesis, and it is also detected in spermatozoa, but it is absent in more immature germ cells, Sertoli cells and interstitial tissue. GLUT8 immunoreactivity is always restricted to the acrosomic system in a manner that matches the acrosome system formation. The GLUT8 expression is mainly associated with the acrosomic membrane in the acrosome, although significant immunoreactivity is also found inside the acrosomic lumen. The specific GLUT8 location suggests that this transporter plays a pivotal role in the fuel supply of spermatozoa, and in the traffic of sugars during the capacitation and fertilization processes.

THE EFFECT OF CERTAIN STILBOESTROL ANALOGUES ON PLASMA PROLACTIN AND TESTOSTERONE IN THE RAT

1973 ◽  
Vol 57 (2) ◽  
pp. 207-215 ◽  
Author(s):  
VARAPAN DANUTRA ◽  
M. E. HARPER ◽  
A. R. BOYNS ◽  
E. N. COLE ◽  
B. G. BROWNSEY ◽  
...  
Keyword(s):  
Biological Effect ◽  
Interstitial Cell ◽  
Adrenal Glands ◽  
Seminal Vesicles ◽  
Plasma Testosterone ◽  
Plasma Prolactin ◽  
Sprague Dawley ◽  
Testosterone Concentration ◽  
Sprague Dawley Rats ◽  
Daily Dose

SUMMARY Oestradiol-17β, diethylstilboestrol (DES), dl-dihydrodibutylstilboestrol (dl-DHBS) and meso-dihydrodibutylstilboestrol (meso-DHBS) were injected intramuscularly into male Sprague-Dawley rats in a daily dose of 100 μg for a period of 10 days. Oestradiol-17β and DES decreased the weight of the prostate and seminal vesicles to the same extent, whereas meso-DHBS was less effective. dl-DHBS was almost inactive. Only oestradiol-17β and DES caused a decrease in the weight of the testes. The adrenal glands increased in weight after administration of either oestradiol-17β, DES or meso-DHBS. Four hormones in the plasma were measured: testosterone, androstenedi-one, prolactin and interstitial cell-stimulating hormone (ICSH). DES decreased the plasma concentration of both ICSH and testosterone. Oestradiol-17β and meso-DHBS administration resulted in a lowering of the plasma testosterone concentration with no effect on ICSH. Oestradiol-17β, DES and meso-DHBS markedly increased plasma prolactin concentrations. dl-DHBS appeared to have little biological effect causing only very small changes in all the parameters investigated.

EFFECT OF FOLLICLE-STIMULATING HORMONE AND INTERSTITIAL CELL-STIMULATING HORMONE ON SPERMATOGENESIS IN LONG-EVANS RATS HYPOPHYSECTOMIZED FOR SIX MONTHS

1963 ◽  
Vol 43 (4) ◽  
pp. 592-600 ◽  
Author(s):  
Ardis J. Lostroh
Keyword(s):  
Germ Cell ◽  
Pituitary Gland ◽  
Germ Cells ◽  
Interstitial Cell ◽  
Follicle Stimulating Hormone ◽  
Primary Spermatocyte ◽  
Seminiferous Tubules ◽  
Effective Agent ◽  
Long Evans ◽  
Stimulating Hormone

ABSTRACT The effects of sheep interstitial cell-stimulating hormone (ICSH) and follicle-stimulating hormone (FSH) on spermatogenesis were studied in rats of the Long-Evans strain six months after removal of the pituitary gland. The results confirm the contention that sheep ICSH alone is not an effective agent for stimulating repair of the epithelium of the seminiferous tubules of the rat, and that preparations of sheep FSH contain a principle which, in the presence of less than 1 μg of ICSH per day, can stimulate substantial repair. All the effects obtained with gonadotrophin preparations can be accomodated if one assumes that FSH is specifically concerned with some critical step in the evolution of the primary spermatocyte and that androgen is responsible for maintaining a favorable intratubular environment in which the germ cells can develop. A complication encountered in this study was an unexpected variation in the germ cell population of rats that had been hypophysectomized for a period of 6 months; the possibility that residual gonadotrophin may account for this observation is discussed.

THE EFFECT OF GROWTH HORMONE AND PROLACTIN PREPARATIONS ON THE CONTROL BY INTERSTITIAL CELL-STIMULATING HORMONE OF UPTAKE OF 65Zn BY THE RAT DORSOLATERAL PROSTATE

1965 ◽  
Vol 32 (2) ◽  
pp. 205-214 ◽  
Author(s):  
S. A. GUNN ◽  
THELMA C. GOULD ◽  
W. A. D. ANDERSON
Keyword(s):  
Growth Hormone ◽  
Interstitial Cell ◽  
Prostate Gland ◽  
Pituitary Hormones ◽  
Thyroid Stimulating Hormone ◽  
Sprague Dawley ◽  
Zn Uptake ◽  
Significant Augmentation ◽  
Consistent Parameter ◽  
Stimulating Hormone

SUMMARY Five pituitary hormones: follicle-stimulating hormone (FSH), adrenocorticotrophic hormone (ACTH), thyroid-stimulating hormone (TSH), growth hormone and prolactin, were tested for their capacity to alter the control by interstitial cell-stimulating hormone (ICSH) of the uptake of 65Zn by the dorsolateral prostate gland of the mature hypophysectomized Sprague-Dawley rat. Only prolactin and growth hormone produced significant augmentation of the response to ICSH. Contamination with growth hormone was apparently not responsible for the augmentation of ICSH activity brought about by the NIH-prolactin preparation used. NIH-prolactin and a highly purified preparation provided by Dr C. H. Li were shown to be equally effective in their capacity to augment the 65Zn-uptake response produced by ICSH. Augmentation was detectable with total doses of prolactin as low as 10–30 μg. (0·21–0·63 i.u.). Prolactin caused an augmentation of testosterone activity on uptake of 65Zn in the hypophysectomized and castrated rat, indicating an effect of prolactin on the prostate that is not mediated by the testis. NIH-growth hormone was not as effective as the prolactin preparations in enhancing ICSH activity, but in sufficient doses produced a significant increase in the 65Zn-uptake response to ICSH. These studies showed also that the uptake of 65Zn of the dorsolateral prostate was a more sensitive and more consistent parameter than glandular weight for the detection of augmentation of ICSH response by growth hormone or prolactin preparations.

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