STUDIES ON GROWTH HORMONE-LIKE (SULPHATION FACTOR) ACTIVITY OF HUMAN SERUM

1962 ◽  
Vol 40 (3_Suppl) ◽  
pp. S58 ◽  
Author(s):  
S. Almqvist
Keyword(s):  
Growth Hormone ◽  
Human Serum ◽  
Factor Activity

Comparison between the human serum growth hormone-binding protein and the water-soluble growth hormone-binding site released from IM-9 lymphocytes

1993 ◽  
Vol 129 (6) ◽  
pp. 559-564 ◽  
Author(s):  
Guy Massa ◽  
Mapoko Ilondo ◽  
Magda Vanderschueren-Lodeweyckx
Keyword(s):  
Growth Hormone ◽  
Human Serum ◽  
Binding Site ◽  
Binding Protein ◽  
Water Soluble ◽  
Hormone Binding ◽  
Serum Growth Hormone ◽  
Gh Receptor ◽  
Growth Hormone Binding Protein ◽  
Hormone Binding Protein

The characteristics of the human serum growth hormone-binding protein (GHBP) were compared with those of a water-soluble GH-binding site prepared by incubating cultured IM-9 lymphocytes in assay buffer with 25 mmol/l iodoacetamide. High-performance liquid chromatography gel filtration of the water-soluble GH-binding site incubated with 125I-labeled human GH ([125I]hGH) revealed a large peak of bound [125I]hGH eluting at the same position as the peak of [125I]hGH bound to the GHBP in serum. The estimated Mr of the peak was 120 000, presumably representing one [125I]hGH bound to two binding sites. The binding specificities of the serum GHBP, the water-soluble GH-binding site and the GH receptor on IM-9 lymphocytes were identical. The binding affinities for 22 000 hGH and for 20 000 hGH of the serum GHBP were similar to the binding affinity of the water-soluble GH-binding site but lower than those of the cellular GH receptor. These findings show that the characteristics of the serum GHBP are comparable to those of the water-soluble GH-binding site released from IM-9 cells and support the hypothesis that in man the serum GHBP is produced by proteolytic cleavage of the cellular GH receptor.

Reduced Serum Somatomedin Activity in Patients with Chronic Liver Disease

1974 ◽  
Vol 47 (4) ◽  
pp. 359-366 ◽  
Author(s):  
A. Wu ◽  
D. B. Grant ◽  
Judy Hambley ◽  
A. J. Levi
Keyword(s):  
Growth Hormone ◽  
Alkaline Phosphatase ◽  
Liver Disease ◽  
Chronic Liver Disease ◽  
Severe Disease ◽  
Chronic Liver ◽  
Hormone Deficiency ◽  
Factor Activity ◽  
Important Site ◽  
Low Serum

1. Somatomedin (SM; sulphation factor) activity was estimated by a chick-cartilage assay in fasting sera from twenty-one patients with chronic liver disease. 2. Low SM values were found in nine out of the ten patients with cirrhosis and in two other patients, one with hepatofibrosis and one with hepatoma. 3. In general, the lowest serum SM activities were found in those patients with the most severe disease and significant correlations were found between serum SM and serum albumin, alkaline phosphatase and bilirubin. 4. Growth hormone was also measured in the samples and concentrations above 10 units/ml were found in seven patients, all of whom had reduced serum SM activities. 5. These findings indicate that low serum SM activity in liver disease is not related to growth hormone deficiency and suggest that the liver may be an important site for SM synthesis in man.

Tissue Factor Activity of Cultured Macrophages. Enhancement by Specific Antigenic Stimulation

1975 ◽  
Author(s):  
S. Beguin ◽  
A. Goutner ◽  
F. Josso
Keyword(s):  
Human Serum ◽  
Tissue Factor ◽  
Cell Activity ◽  
Cell Suspensions ◽  
Rabbit Serum ◽  
Factor Activity ◽  
Clotting System ◽  
Subsequent Increase ◽  
Macrophage Activity ◽  
Tissue Factor Activity

Peritoneal macrophage suspensions (95 p. cent pure) were obtained in rabbits either intact or previously immunized against human serum. Cell suspensions exhibited a clear tissue factor activity, closely related to the cell concentration.Macrophages (10000/mm3) were cultured in the presence of PO4)2 Ca3 adsorbed and decomplemented serum of either rabbit or human origin. Clotting activity of the supernatant medium was tested after 24 hour culture. In immunized animals, tissue factor activity was much higher when the cells were cultured in the presence of the antigens (human serum) instead of rabbit serum; no difference was observed in intact rabbits.Contamination of the cell suspensions by lymphocytes seems too low to play a role in this stimulation of macrophage activity. The reported data suggest that antibodies bound to macrophage membrane react with the medium antigens with subsequent increase of the cell activity.Such a phenomenon could initiate the activation of the clotting system observed in some immune reactions.

SULPHATION FACTOR (SOMATOMEDIN ACTIVITY) IN EXPERIMENTAL PROTEIN MALNUTRITION IN THE RAT

1978 ◽  
Vol 77 (2) ◽  
pp. 233-240 ◽  
Author(s):  
B. SHAPIRO ◽  
B. L. PIMSTONE
Keyword(s):  
Growth Hormone ◽  
Rat Model ◽  
Protein Malnutrition ◽  
Bovine Growth Hormone ◽  
Factor Activity ◽  
Low Level

SUMMARY In a rat model of protein malnutrition in which the failure of growth is a major feature, a low level of bioassayable sulphation factor activity was present in the serum, associated with normal levels of growth hormone and low insulin in the plasma. The administration of pharmacological doses of human or bovine growth hormone did not increase the amount of sulphation factor activity in the serum or the width of the tibial epiphyses in the proteinmalnourished animals. The basal and serum-stimulated incorporation of 35SO42− into the costal cartilages of malnourished animals did not differ from that of controls, which suggests that the responsiveness of the end-organs was normal.

EFFECT OF INTRAVENOUS ADMINISTRATION OF HUMAN GROWTH HORMONE ON SULPHATION FACTOR ACTIVITY IN SERUM OF HYPOPITUITARY SUBJECTS

1971 ◽  
Vol 66 (3) ◽  
pp. 491-497 ◽  
Author(s):  
Kerstin Hall
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Intravenous Administration ◽  
Human Growth ◽  
Significant Rise ◽  
Mean Difference ◽  
Factor Activity ◽  
The Mean

ABSTRACT Human growth hormone (HGH) administered as an iv injection of 2–4 mg to hypopituitary patients induced a rise in the levels of sulphation factor (SF) in serum. The low basal levels of SF were not changed during the first hour after HGH injection. Not until three hours after injection, when HGH values approached basal values, there was a significant rise in SF. The mean difference of SF at one and at three hours after HGH injection was 0.52 ± 0.11.

Brain Growth-Promoting Activity in Human Serum: Relationship to Growth Hormone and Somatomedin

1978 ◽  
Vol 47 (5) ◽  
pp. 1129-1131 ◽  
Author(s):  
VICKI R. SARA ◽  
MARGARET C. STUART ◽  
R. RUTHERFORD ◽  
S. MOORE ◽  
L. LAZARUS
Keyword(s):  
Growth Hormone ◽  
Human Serum ◽  
Brain Growth ◽  
Growth Promoting

NON-SUPPRESSIBLE INSULIN-LIKE ACTIVITY IN LARON'S SYNDROME

1979 ◽  
Vol 90 (3) ◽  
pp. 414-420 ◽  
Author(s):  
Knud W. Kastrup ◽  
Jürgen Zapf
Keyword(s):  
Growth Hormone ◽  
Growth Factors ◽  
Growth Retardation ◽  
Binding Assay ◽  
Factor Activity ◽  
Protein Binding Assay ◽  
The Family ◽  
Low Activity ◽  
Severe Growth ◽  
Insulin Like Activity

ABSTRACT Severe growth retardation is found in patients with high levels of growth hormone and low sulphation factor activity or somatomedin. Also nonsuppressible insulin-like activity (NSILA-s) has been found to be very low in a patient with this condition as measured by bioassay, protein binding assay and radioimmunoassay and to be below activities found in hypopituitary patients. Partially purified NSILA-s restored the ability of serum to increase sulphation activity although full restitution may still depend on other factors. These findings support the hypothesis that NSILA-s belongs to the family of somatomedin and thus is involved in promoting growth, and that low activity of these growth factors is a primary cause of the growth retardation found in these patients.

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