URINARY EXCRETION OF ADRENAL HORMONES IN GONADAL DYSGENESIS AND ITS RESPONSE TO CORTICOTROPHIN AND GONADOTROPHIN STIMULATION

1962 ◽  
Vol 40 (2) ◽  
pp. 247-253 ◽  
Author(s):  
M. L. Batrinos ◽  
B. Mathieu de Fossey ◽  
P. Aubert
Keyword(s):  
Urinary Excretion ◽  
Gonadal Dysgenesis ◽  
Normal Subjects ◽  
Chorionic Gonadotrophin ◽  
Adrenal Hormones ◽  
Normal Limits ◽  
Sex Chromatin ◽  
Male Sex ◽  
Hcg Test ◽  
Secretory Capacity

ABSTRACT The functional capacity of the adrenals was studied in eight adult patients with gonadal dysgenesis, ranging in age from 16–42 years, by measuring the 24-hour urinary excretion of 17-hydroxycorticosteroids (17-OHCS) and of 17-ketosteroids (17-KS) with the patient at rest and also by estimating its response to stimulation with corticotrophin and chorionic gonadotrophin. In six patients the clinical diagnosis of the syndrome was confirmed by laparotomy, when no gonads were found in five patients and a white fibrous band replacing the gonads was found in the other. The remaining two patients of the series both had male sex-chromatin. The resting excretion of both 17-OHCS and 17-KS was within normal limits in all eight patients. After corticotrophin stimulation (25 IU infused over 8 hours) an increased 17-OHCS excretion was found in all patients but the increase was less constant for the 17-KS. These results are identical with those expected from normal subjects and were considered to indicate a normal adrenal secretory capacity in the present cases. Stimulation by chorionic gonadotrophin (HCG test) was performed in four patients using various doses of the hormone, namely 1500 IU for 5 days, 5000 IU for 2 or 5 days, or 10 000 IU for 2 days. Three of these patients had no gonads and the fourth had male sex-chromatin. The HCG test failed to elicit an increased excretion of either 17-OHCS or 17-KS in these patients. This absence of response is regarded as evidence that HCG does not stimulate adrenal tissue to secrete the precursors of these hormones. The findings of the present study are discussed in the light of previous publications.

URINARY EXCRETION OF ANDROST-16-EN-3α-OL LEVELS IN NORMAL SUBJECTS, AND EFFECTS OF TREATMENT WITH TROPHIC HORMONES

1962 ◽  
Vol 24 (4) ◽  
pp. 435-444 ◽  
Author(s):  
B. W. L. BROOKSBANK
Keyword(s):  
Menstrual Cycle ◽  
Urinary Excretion ◽  
Small Proportion ◽  
Intramuscular Injection ◽  
Human Subjects ◽  
Normal Subjects ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Normal Human ◽  
The Mean

SUMMARY Data are presented on the urinary excretion of androst-16-en-3α-ol by normal human subjects over the age span 4–86 years. The figures range from < 100 to 2630 μg./24 hr. in males, and < 100 to 1100 μg./24 hr. in females, the mean for men of 16–45 years being nearly three times that for women of the same age. The effect on urinary androstenol and 17-oxosteroids of human chorionic gonadotrophin (HCG) and of corticotrophin (ACTH) have been compared in three normal young men and two women. Marked elevation of androstenol excretion occurred after ACTH in both sexes, while HCG administration resulted in an increased urinary output of androstenol and 17-oxosteroids only in two of the men and not in the women tested in either phase of the menstrual cycle. Intramuscular injection of androstenol itself (20 mg.) resulted in increased levels of androstenol in the urine equivalent only to a very small proportion of the injected dose. The metabolic origin of androstenol is discussed in the light of the results presented and of those of other investigators. It seems likely that androstenol arises not primarily from testosterone but mainly from an adrenal precursor.

Deoxycorticosterone Excretion in Normal, Hypertensive and Hypokalaemic Subjects

1975 ◽  
Vol 48 (2) ◽  
pp. 97-105 ◽  
Author(s):  
C. L. Cope ◽  
S. Loizou
Keyword(s):  
Essential Hypertension ◽  
Urinary Excretion ◽  
Cushing’S Syndrome ◽  
Normal Subjects ◽  
Cushing's Syndrome ◽  
Metabolic Disturbance ◽  
Normal Limits ◽  
Excretion Rates

1. Radioimmunoassay has been used to detect and estimate the urinary excretion of deoxycorticosterone (DOC) in normal, hypertensive and hypokalaemic subjects. The range of excretions in ten healthy normal subjects was 41–232 pmol (13.7–76.7 ng) daily, with a mean of 124 pmol (41 ng). 2. In fourteen subjects with essential hypertension without metabolic disturbance the range found was 29–144 pmol (9.7–47.7 ng) daily, with a mean of 87 pmol (28.8 ng), which is not significantly different from that in normal subjects. 3. In twelve patients with Cushing's syndrome due to adrenal cortical hyperplasia the range found was 26–542 pmol (8.7–179 ng). Ten of these twelve patients had values within normal limits. 4. Of nine subjects showing hypokalaemia, eight had elevated excretion of deoxycorticosterone with values from 263 to 5515 pmol (87–1820 ng) daily. Seven of these were hypertensive and two were normotensive. The elevated excretion of deoxycorticosterone found in hypokalaemic subjects is thus not confined to those with hypertension. 5. No correlation has been found between excretion rates for aldosterone and deoxycorticosterone. Raised excretion of the latter provides an indicator of disturbed adrenal cortical metabolism.

Spontaneous Haemophilia in a Female

1960 ◽  
Vol 04 (03) ◽  
pp. 369-375 ◽  
Author(s):  
E. H Braun ◽  
David B. Stollar
Keyword(s):  
Family History ◽  
Bleeding Time ◽  
Dental Extraction ◽  
White Girl ◽  
Complete Family ◽  
Normal Limits ◽  
Sex Chromatin ◽  
History Of ◽  
Generation Test

SummaryA case of haemophilia in a young white girl is described. There was a history of bleeding from birth. The thromboplastin generation test was grossly abnormal and A. H. G. levels were below 1%. Bleeding time and capillary morphology was within normal limits. Dental extraction after transfusion caused almost uncontrollable haemorrhage.A complete family history was obtained for four generations. There was no case of a “bleeder” amongst these.The girl’s apparent sex was confirmed by sex chromatin studies.

ÉTUDES IN VIVO SUR LE MÉTABOLISME DES ANDROGÉNES APRES ADMINISTRATION DE STÉROÏDES INHIBITEURS DE L'OVULATION

1965 ◽  
Vol 50 (1) ◽  
pp. 131-144 ◽  
Author(s):  
P. Mauvais-Jarvis ◽  
M. F. Jayle ◽  
J. Decourt ◽  
J. Louchart ◽  
J. Truffert
Keyword(s):  
Luteinizing Hormone ◽  
Urinary Excretion ◽  
Normal Subjects ◽  
Hormone Activity ◽  
Testosterone Production ◽  
Normal Men ◽  
Ethinyl Oestradiol

ABSTRACT Normal subjects and hirsute women with micropolycystic ovaries were treated with ethinyl-oestrenol + 3-methoxy-ethinyl-oestradiol (Lyndiol®), in view of studying the action of this compound on the production of androgens and on the urinary excretion of their metabolites. In normal men, the production of testosterone and the excretion of androsterone and aetiocholanolone are suppressed, whereas the excretion of other 17-ketosteroids and the production of dehydroepiandrosterone sulphate are unchanged. Moreover, the luteinizing hormone activity (LH) in plasma is depressed. It seems that the preparation inhibits specifically the testicular androgen production, by suppressing the hypothalamo-hypophyseal control of LH. Testosterone production and urinary 17-ketosteroid excretion are modified in the same way in women with Stein-Leventhal's syndrome. Physiopathological and therapeutical implications which come from these results are discussed.

A COMPARISON OF TWO METHODS FOR MEASUREMENT OF ALDOSTERONE SECRETION RATE IN MAN

1966 ◽  
Vol 53 (2) ◽  
pp. 177-188 ◽  
Author(s):  
P. Lund-Johansen ◽  
T. Thorsen ◽  
K. F. Støa
Keyword(s):  
Urinary Excretion ◽  
Normal Subjects ◽  
Secretion Rate ◽  
Aldosterone Secretion ◽  
Simple Method ◽  
Mean Values ◽  
Pathological Conditions ◽  
Significant Difference ◽  
Derivative Method ◽  
The Mean

ABSTRACT A comparison has been made between (A), a relatively simple method for the measurement of aldosterone secretion rate, based on paper chromatography and direct densitometry of the aldosterone spot and (B) a more elaborate isotope derivative method. The mean secretion rate in 9 normal subjects was 112 ± 26 μg per 24 hours (method A) and 135 ± 35 μg per 24 hours (method B). The »secretion rate« in one adrenalectomized subject after the intravenous injection of 250 μg of aldosterone was 230 μg per 24 hours (method A) and 294 μg per 24 hours (method B). There was no significant difference in the mean values, and correlation between the two methods was good (r = 0.80). It is concluded that the densitometric method is suitable for clinical purposes as well as research, being more rapid and less expensive than the isotope derivative method. Method A also measures the urinary excretion of the aldosterone 3-oxo-conjugate, which is of interest in many pathological conditions. The densitometric method is obviously the less sensitive and a prerequisite for its use is an aldosterone secretion of 20—30 μg per 24 hours. Lower values are, however, rare in adults.

THE INFLUENCE OF 17α-METHYL-19-NORTESTOSTERONE ON THE ENDOCRINE FUNCTION OF THE ADRENAL CORTEX

1960 ◽  
Vol XXXIII (III) ◽  
pp. 388-400 ◽  
Author(s):  
L. G. Huis in 't Veld ◽  
B. Louwerens ◽  
P. A. F. van der Spek
Keyword(s):  
Urinary Excretion ◽  
Adrenal Cortex ◽  
Direct Effect ◽  
Chorionic Gonadotrophin ◽  
Endocrine Function ◽  
List Type ◽  
Prolonged Administration ◽  
Acth Secretion ◽  
Male Patients ◽  
Normal Males

ABSTRACT In two male patients and two castrated males, the influence of corticotrophin (ACTH) on the urinary excretion of neutral 17-ketosteroids and 17-hydroxycorticosteroids was determined before and during a period in which patients were treated with 5 mg 17α-methyl-19-nortestosterone (MNT) daily. In two castrated males, moreover, the influence of chorionic gonadotrophin and ACTH + chorionic gonadotrophin on the urinary excretion of 17-ketosteroids and 17-hydroxycorticosteroids was determined before and during a period of treatment with 5 mg MNT daily. Prolonged administration of MNT causes a decrease in the urinary excretion of neutral 17-ketosteroids and 17-hydroxycorticosteroids both in the normal males and in the male castrates. ACTH caused an increase in the urinary excretion of 17-ketosteroids and 17-hydroxycorticosteroids before and during MNT administration. During MNT administration this increase (expressed in mg/24 hours) was ≤ the increase produced by the same dose of ACTH prior to MNT administration. In two male castrates treated with MNT, chorionic gonadotrophin caused no increase in the urinary excretion of 17-ketosteroids and 17-hydroxycorticosteroids. The effect obtained before and during MNT administration by administration of ACTH + chorionic gonadotrophin did not exceed the effect obtained by the same dose of ACTH alone. Our conclusion is that the effect of MNT on the excretion of adrenocortical steroids is not due to the inhibition of the ACTH secretion. The possibility of a direct effect of MNT on the adrenal cortex has not been excluded with complete certainty. A change in the corticosteroid metabolism due to the influence of MNT, however, must also be taken into consideration.

Identification of N-Monoacetylcystine in Uraemic Plasma

1981 ◽  
Vol 60 (3) ◽  
pp. 331-334 ◽  
Author(s):  
F. Gejyo ◽  
G. Ito ◽  
Y. Kinoshita
Keyword(s):  
Plasma Levels ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Exchange Chromatography ◽  
Cysteic Acid ◽  
Homocysteic Acid ◽  
Normal Limits ◽  
Acidic Nature ◽  
Sulphur Amino Acids

1. An unidentified ninhydrin-positive substance of an acidic nature was detected in the plasma of uraemic patients. This substance was isolated from haemodialysate by ion-exchange chromatography and gel filtration, and identified as a sulphur-containing amino acid: N-monoacetylcystine. 2. The quantitative determination of sulphur amino acids in plasma revealed that the plasma levels of cysteic acid, homocysteic acid, taurine, cystine and cystathionine as well as N-monoacetylcystine in uraemic patients were markedly higher than in normal subjects (P < 0.001 for each). However, the plasma levels of methionine in uraemic patients were within normal limits.

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