scholarly journals Method of X-Ray Anomalous Diffraction for Lipid Structures

2006 ◽  
Vol 91 (2) ◽  
pp. 736-743 ◽  
Author(s):  
Wangchen Wang ◽  
Deng Pan ◽  
Yang Song ◽  
Wenhan Liu ◽  
Lin Yang ◽  
...  
Keyword(s):  
Anomalous Diffraction ◽  
X Ray ◽  
Lipid Structures

scholarly journals Semi-automated protein crystal mounting device for the sulfur single-wavelength anomalous diffraction method

2010 ◽  
Vol 43 (2) ◽  
pp. 341-346 ◽  
Author(s):  
Yu Kitago ◽  
Nobuhisa Watanabe ◽  
Isao Tanaka
Keyword(s):  
Diffraction Method ◽  
Systematic Errors ◽  
Protein Crystal ◽  
X Rays ◽  
Anomalous Diffraction ◽  
X Ray ◽  
Frozen Solution ◽  
Custom Made ◽  
Protein Molecules ◽  
X Ray Absorption

Use of longer-wavelength X-rays has advantages for the detection of small anomalous signals from light atoms, such as sulfur, in protein molecules. However, the accuracy of the measured diffraction data decreases at longer wavelengths because of the greater X-ray absorption. The capillary-top mounting method (formerly the loopless mounting method) makes it possible to eliminate frozen solution around the protein crystal and reduces systematic errors in the evaluation of small anomalous differences. However, use of this method requires custom-made tools and a large amount of skill. Here, the development of a device that can freeze the protein crystal semi-automatically using the capillary-top mounting method is described. This device can pick up the protein crystal from the crystallization drop using a micro-manipulator, and further procedures, such as withdrawal of the solution around the crystal by suction and subsequent flash freezing of the protein crystal, are carried out automatically. This device makes it easy for structural biologists to use the capillary-top mounting method for sulfur single-wavelength anomalous diffraction phasing using longer-wavelength X-rays.

X-ray anomalous diffraction and resonant magnetic scattering X-ray study of FexMn1−x/Ir(001) superlattices

1997 ◽  
Vol 165 (1-3) ◽  
pp. 87-91 ◽  
Author(s):  
A. Déchelette-Barbara ◽  
J.M. Tonnerre ◽  
M.C. Saint-Lager ◽  
F. Bartolomé ◽  
J.F. Bérar ◽  
...  
Keyword(s):  
Magnetic Scattering ◽  
Anomalous Diffraction ◽  
X Ray

A method to stabilize the incident X-ray energy for anomalous diffraction measurements

2017 ◽  
Vol 24 (4) ◽  
pp. 781-786
Author(s):  
Wenjia Wang ◽  
Xiaoyun Yang ◽  
Guangcai Chang ◽  
Pengfei An ◽  
Kewen Cha ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Data Collection ◽  
Absorption Edge ◽  
Feedback Loop ◽  
Inflection Point ◽  
Single Crystal Diffraction ◽  
Double Crystal ◽  
Anomalous Diffraction ◽  
X Ray ◽  
Double Crystal Monochromator

A method to calibrate and stabilize the incident X-ray energy for anomalous diffraction data collection is provided and has been successfully used at the single-crystal diffraction beamline 1W2B at the Beijing Synchrotron Radiation Facilities. Employing a feedback loop to control the movement of the double-crystal monochromator, this new method enables the incident X-ray energy to be kept within a 0.2 eV range at the inflection point of the absorption edge.

scholarly journals Multi-crystal native SAD analysis at 6 keV

2014 ◽  
Vol 70 (10) ◽  
pp. 2544-2557 ◽  
Author(s):  
Qun Liu ◽  
Youzhong Guo ◽  
Yanqi Chang ◽  
Zheng Cai ◽  
Zahra Assur ◽  
...  
Keyword(s):  
De Novo ◽  
Real Life ◽  
Kinase Domain ◽  
X Rays ◽  
Protein Kinase Domain ◽  
Anomalous Diffraction ◽  
X Ray ◽  
Tyrosine Protein Kinase ◽  
Sad Phasing ◽  
Feasibility Test

Anomalous diffraction signals from typical native macromolecules are very weak, frustrating their use inde novostructure determination. Here, native SAD procedures are described to enhance signal to noise in anomalous diffraction by using multiple crystals in combination with synchrotron X-rays at 6 keV. Increased anomalous signals were obtained at 6 keV compared with 7 keV X-ray energy, which was used for previous native SAD analyses. A feasibility test of multi-crystal-based native SAD phasing was performed at 3.2 Å resolution for a known tyrosine protein kinase domain, and real-life applications were made to two novel membrane proteins at about 3.0 Å resolution. The three applications collectively serve to validate the robust feasibility of native SAD phasing at lower energy.

scholarly journals Selenium single-wavelength anomalous diffraction de novo phasing using an X-ray-free electron laser

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Mark S. Hunter ◽  
Chun Hong Yoon ◽  
Hasan DeMirci ◽  
Raymond G. Sierra ◽  
E. Han Dao ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
De Novo ◽  
Anomalous Diffraction ◽  
X Ray

X-Ray Anomalous Diffraction Study of the Crystal Structure of Y(Co,Ni)4B Compounds

2001 ◽  
Vol 378-381 ◽  
pp. 434-439 ◽  
Author(s):  
Cyril Chacon ◽  
Olivier Isnard ◽  
Jean François Berar
Keyword(s):  
Crystal Structure ◽  
Diffraction Study ◽  
Anomalous Diffraction ◽  
X Ray
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