miR-200c-141 Enhances Sheep Kidney Cell Reprogramming into Pluripotent Cells by Targeting ZEB1

Human Kidney Cell Reprogramming: Applications for Disease Modeling and Personalized Medicine

Journal of the American Society of Nephrology ◽

10.1681/asn.2012121199 ◽

2013 ◽

Vol 24 (9) ◽

pp. 1347-1356 ◽

Cited By ~ 15

Author(s):

Adam C. O’Neill ◽

Sharon D. Ricardo

Keyword(s):

Personalized Medicine ◽

Kidney Cell ◽

Disease Modeling ◽

Human Kidney ◽

Cell Reprogramming ◽

And Personalized Medicine

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Analysis of the Epigenetic Signature of Cell Reprogramming by Computational DNA Methylation Profiles

Current Bioinformatics ◽

10.2174/1574893614666190919103752 ◽

2020 ◽

Vol 15 (6) ◽

pp. 589-599

Author(s):

Yongchun Zuo ◽

Mingmin Song ◽

Hanshuang Li ◽

Xing Chen ◽

Pengbo Cao ◽

...

Keyword(s):

Dna Methylation ◽

Molecular Mechanism ◽

Regional Distribution ◽

Dermal Fibroblasts ◽

Cell Reprogramming ◽

Dna Hypermethylation ◽

Pluripotent Cells ◽

Cpg Sites ◽

Hdf Cells ◽

Insight Into

Background: DNA methylation plays an important role in the reprogramming process. Understanding the underlying molecular mechanism of reprogramming is crucial for answering fundamental questions regarding the transition of cell identity. Methods: In this study, based on the genome-wide DNA methylation data from different cell lines, comparative methylation profiles were proposed to identify the epigenetic signature of cell reprogramming. Results: The density profile of CpG methylation showed that pluripotent cells are more polarized than Human Dermal Fibroblasts (HDF) cells. The heterogeneity of iPS has a greater deviation in the DNA hypermethylation pattern. The result of regional distribution showed that the differential CpG sites between pluripotent cells and HDFs tend to accumulate in the gene body and CpG shelf regions, whereas the internal differential methylation CpG sites (DMCs) of three types of pluripotent cells tend to accumulate in the TSS1500 region. Furthermore, a series of endogenous markers of cell reprogramming were identified based on the integrative analysis, including focal adhesion, pluripotency maintenance and transcription regulation. The calcium signaling pathway was detected as one of the signatures between NT cells and iPS cells. Finally, the regional bias of DNA methylation for key pluripotency factors was discussed. Our studies provide new insight into the barrier identification of cell reprogramming. Conclusion: Our studies analyzed some epigenetic markers and barriers of nuclear reprogramming, hoping to provide new insight into understanding the underlying molecular mechanism of reprogramming.

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Mitochondrial Dynamics: In Cell Reprogramming as It Is in Cancer

Stem Cells International ◽

10.1155/2017/8073721 ◽

2017 ◽

Vol 2017 ◽

pp. 1-11 ◽

Cited By ~ 17

Author(s):

Javier Prieto ◽

Josema Torres

Keyword(s):

Stem Cells ◽

Pluripotent Stem Cells ◽

Mitochondrial Dynamics ◽

Mitochondrial Fission ◽

Somatic Cells ◽

Embryonic Stem ◽

Cellular Transformation ◽

Cell Reprogramming ◽

Mitochondrial Morphology ◽

Pluripotent Cells

Somatic cells can be reprogrammed into a pluripotent cellular state similar to that of embryonic stem cells. Given the significant physiological differences between the somatic and pluripotent cells, cell reprogramming is associated with a profound reorganization of the somatic phenotype at all levels. The remodeling of mitochondrial morphology is one of these dramatic changes that somatic cells have to undertake during cell reprogramming. Somatic cells transform their tubular and interconnected mitochondrial network to the fragmented and isolated organelles found in pluripotent stem cells early during cell reprogramming. Accordingly, mitochondrial fission, the process whereby the mitochondria divide, plays an important role in the cell reprogramming process. Here, we present an overview of the importance of mitochondrial fission in both cell reprogramming and cellular transformation.

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