scholarly journals Intratracheal Transplantation of Amnion-Derived Mesenchymal Stem Cells Ameliorates Hyperoxia-Induced Neonatal Hyperoxic Lung Injury via Aminoacyl-Peptide Hydrolase

2020 ◽  
Vol 13 (2) ◽  
pp. 221-236
Author(s):  
Zhenghao Li ◽  
Xiangcui Gong ◽  
Dong Li ◽  
Xiaofei Yang ◽  
Qing Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Lung Injury ◽  
Peptide Hydrolase ◽  
Hyperoxic Lung Injury

scholarly journals The therapeutic effect of mesenchymal stem cells on pulmonary myeloid cells following neonatal hyperoxic lung injury in mice

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Ali Al-Rubaie ◽  
Andrea F. Wise ◽  
Foula Sozo ◽  
Robert De Matteo ◽  
Chrishan S. Samuel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Lung Injury ◽  
Therapeutic Effect ◽  
Myeloid Cells ◽  
Hyperoxic Lung Injury

Mesenchymal stem cells protect against neonatal rat hyperoxic lung injury

2013 ◽  
Vol 13 (6) ◽  
pp. 817-829 ◽  
Author(s):  
Hongshan Zhang ◽  
Jianpei Fang ◽  
Yanyun Wu ◽  
Yougang Mai ◽  
Wenyu Lai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Lung Injury ◽  
Neonatal Rat ◽  
Hyperoxic Lung Injury

scholarly journals Soluble PTX3 of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Attenuates Hyperoxic Lung Injury by Activating Macrophage Polarization in Neonatal Rat Model

2020 ◽  
Vol 2020 ◽  
pp. 1-18 ◽  
Author(s):  
Miyeon Kim ◽  
Ji Hye Kwon ◽  
Yun Kyung Bae ◽  
Gee-Hye Kim ◽  
Soyoun Um ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Lung Injury ◽  
Therapeutic Potential ◽  
Macrophage Polarization ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Therapeutic Effects ◽  
Paracrine Action ◽  
Hyperoxic Lung Injury

Therapeutic treatment of various inflammation-related diseases using mesenchymal stem cells (MSCs) has increased in recent years because of the paracrine action of these cells but shows several limitations. First, MSC-based therapies exhibit varying efficacies; thus, biomarkers should be determined to identify who may benefit from these candidate therapeutic agents. Second, the mechanism underlying the therapeutic effects is poorly understood. To evaluate the effects of human umbilical cord blood-derived MSCs (UCB-MSCs) on macrophages, the macrophage cell line NR8383 stimulated with lipopolysaccharide (LPS) was cocultured by UCB-MSCs. We found that UCB-MSCs mediated changes in macrophage polarization towards M2 from M1 macrophages. To identify the paracrine action underlying the anti-inflammation effect of UCB-MSCs, the secretion of UCB-MSCs exposed to LPS-stimulated NR8383 cells was tested using a biotin label-based 507 antibody array. Among the secreted proteins, we selected pentraxin-related protein PTX3/tumor necrosis factor-inducible gene 14 protein (PTX3) to investigate its association with UCB-MSCs in macrophage polarization. We found that human PTX3 was secreted from UCB-MSCs under inflammation condition and reinforced the M2 macrophage marker via the Dectin-1 receptor by activating MSK1/2 phosphorylation signaling in NR8383 cells. Accordingly, knockdown of PTX3 in UCB-MSCs significantly attenuated their therapeutic effects in a neonatal hyperoxic lung injury resulting in reduced survival, lung alveolarization, M2 marker expression, Dectin-1 levels, anti-inflammatory cytokines, and improved M1 marker expression and inflammatory cytokines compared to control MSC-injected rats. UCB-MSCs show therapeutic potential by controlling macrophage polarization. Interestingly, higher PTX3 levels in UCB-MSCs induced greater improvement in the therapeutic effects than lower PTX3 levels. Collectively, PTX3 is a potential marker with critical paracrine effects for predicting the therapeutic potential of MSC therapy in inflammatory diseases; quality control assessments using PTX3 may be useful for improving the therapeutic effects of UCB-MSCs.

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