scholarly journals Enhancement of Replication and Differentiation Potential of Human Bone Marrow Stem Cells by Nicotinamide Treatment

2018 ◽  
Vol 11 (1) ◽  
pp. 13-25 ◽  
Author(s):  
Jeong Su Ok ◽  
Seon Beom Song ◽  
Eun Seong Hwang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Nicotinamide Treatment

scholarly journals Human dental pulp stem cells exhibit enhanced properties in comparison to human bone marrow stem cells on neurites outgrowth

2020 ◽  
Vol 34 (4) ◽  
pp. 5499-5511 ◽  
Author(s):  
Pierfrancesco Pagella ◽  
Shayee Miran ◽  
Estrela Neto ◽  
Ivan Martin ◽  
Meriem Lamghari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Dental Pulp ◽  
Enhanced Properties

scholarly journals Osteoporosis is accompanied by reduced CD274 expression in human bone marrow-derived mesenchymal stem cells

2021 ◽  
Vol 41 ◽  
pp. 603-615
Author(s):  
A-N Zeller ◽  
◽  
M Selle ◽  
Z Gong ◽  
M Winkelmann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Surface ◽  
Chondrogenic Differentiation ◽  
Surface Antigens ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Proliferation Potential

Underlying pathomechanisms of osteoporosis are still not fully elucidated. Cell-based therapy approaches pose new possibilities to treat osteoporosis and its complications. The aim of this study was to quantify differences in human bone marrow-derived mesenchymal stem cells (hBMSCs) between healthy donors and those suffering from clinically manifest osteoporosis. Cell samples of seven donors for each group were selected retrospectively from the hBMSC cell bank of the Trauma Department of Hannover Medical School. Cells were evaluated for their adipogenic, osteogenic and chondrogenic differentiation potential, for their proliferation potential and expression of surface antigens. Furthermore, a RT2 Osteoporosis Profiler PCR array, as well as quantitative real-time PCR were carried out to evaluate changes in gene expression. Cultivated hBMSCs from osteoporotic donors showed significantly lower cell surface expression of CD274 (4.98 % ± 2.38 %) than those from the control group (26.03 % ± 13.39 %; p = 0.007), as assessed by flow cytometry. In osteoporotic patients, genes involved in inhibition of the anabolic WNT signalling pathway and those associated with stimulation of bone resorption were significantly upregulated. Apart from these changes, no significant differences were found for the other cell surface antigens, adipogenic, osteogenic and chondrogenic differentiation ability as well as proliferation potential. These findings supported the theory of an influence of CD274 on the regulation of bone metabolism. CD274 might be a promising target for further investigations of the pathogenesis of osteoporosis and of cell-based therapies involving MSCs.

scholarly journals The Role of Mitochondria in the Dual Effect of Low-Temperature Plasma on Human Bone Marrow Stem Cells: From Apoptosis to Activation of Cell Proliferation

2020 ◽  
Vol 10 (24) ◽  
pp. 8971
Author(s):  
Sergej V. Belov ◽  
Yakov P. Lobachevsky ◽  
Yurij K. Danilejko ◽  
Aleksej B. Egorov ◽  
Alexander V. Simakin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Low Temperature ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Low Temperature Plasma ◽  
Dual Effect ◽  
Temperature Plasma

The potential use of low-temperature plasma (LTP) for therapeutic purposes has aroused the concern of many researchers. This paper examines the effect of LTP on the morphofunctional state of human bone marrow stem cells (BMSC). It has been established that LTP-induced oxidative stress has a dual effect on the state of stem cells. On the one hand, a cell culture exposed to LTP exhibits the progression of a destructive processes, which is manifested by the perturbation of the cell’s morphology, the initiation of lipid peroxidation and the accumulation of products of this process, like diene conjugates and malondialdehyde, as well as the induction of mitochondrial dysfunction, leading to cell death. On the other hand, the effect of LTP on BMSC located at a distance from the plasma is accompanied by the activation of proliferative processes, as evidenced by the tendency of the activity of mitochondrial biogenesis and fission/fusion processes to increase. The paper discusses the role of mitochondria and reactive oxygen species (ROS) in the cellular response to LTP.

scholarly journals Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 370
Author(s):  
Alessio Rochira ◽  
Luisa Siculella ◽  
Fabrizio Damiano ◽  
Andrea Palermo ◽  
Franco Ferrante ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Protein Quantification ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Medium ◽  
Alizarin Red ◽  
Matrix Mineralization

Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.

Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity

Cytotherapy ◽  
2009 ◽  
pp. 1-15 ◽  
Author(s):  
Remco Koninckx ◽  
Karen Hensen ◽  
Annick Daniëls ◽  
Marjan Moreels ◽  
Ivo Lambrichts ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Neonatal Rat ◽  
Human Bone Marrow ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

scholarly journals The Effects of Extracellular pH on Proliferation and Differentiation of human Bone Marrow Stem Cells

2012 ◽  
Vol 19 (1) ◽  
pp. 35 ◽  
Author(s):  
Yea Hyun Leem ◽  
Tae Suk Nam ◽  
Jung Hwa Kim ◽  
Kang Sik Lee ◽  
Dong Ho Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Extracellular Ph ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Proliferation And Differentiation

Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

Methods ◽  
2008 ◽  
Vol 45 (1) ◽  
pp. 2-9 ◽  
Author(s):  
Simon R. Tew ◽  
Alan D. Murdoch ◽  
Richard P. Rauchenberg ◽  
Timothy E. Hardingham
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Chondrocytes
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