scholarly journals Bone Marrow Stem Cells Anti-liver Fibrosis Potency: Inhibition of Hepatic Stellate Cells Activity and Extracellular Matrix Deposition

2017 ◽  
Vol 10 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Ervina Julien Sitanggang ◽  
Radiana Dhewayani Antarianto ◽  
Sri Widia A. Jusman ◽  
Jeanne Adiwinata Pawitan ◽  
Ahmad Aulia Jusuf
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Liver Fibrosis ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Bone Marrow Stem Cells ◽  
Matrix Deposition ◽  
Extracellular Matrix Deposition

Bone Marrow Stem Cells Contribute to Alcohol Liver Fibrosis in Humans

2010 ◽  
Vol 19 (9) ◽  
pp. 1417-1425 ◽  
Author(s):  
Evangelos Dalakas ◽  
Philip N. Newsome ◽  
Shelagh Boyle ◽  
Rachael Brown ◽  
Anne Pryde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Fibrosis ◽  
Bone Marrow Stem Cells

scholarly journals Human placental mesenchymal stem cells ameliorate liver fibrosis in mice by upregulation of Caveolin1 in hepatic stellate cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yunqi Yao ◽  
Zhemin Xia ◽  
Fuyi Cheng ◽  
Qingyuan Jang ◽  
Jiao He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Fibrosis ◽  
Therapeutic Effect ◽  
Hepatic Stellate Cells ◽  
Time Window ◽  
Stellate Cells ◽  
Therapeutic Benefits ◽  
Placental Mesenchymal Stem Cells

Abstract Background Liver fibrosis (LF) is a common pathological process characterized by the activation of hepatic stellate cells (HSCs) and accumulation of extracellular matrix. Severe LF causes cirrhosis and even liver failure, a major cause of morbidity and mortality worldwide. Transplantation of human placental mesenchymal stem cells (hPMSCs) has been considered as an alternative therapy. However, the underlying mechanisms and the appropriate time window for hPMSC transplantation are not well understood. Methods We established mouse models of CCl4-injured LF and administered hPMSCs at different stages of LF once a week for 2 weeks. The therapeutic effect of hPMSCs on LF was investigated, according to histopathological and blood biochemical analyses. In vitro, the effect of hPMSCs and the secretomes of hPMSCs on the inhibition of activated HSCs was assessed. RNA sequencing (RNA-seq) analysis, real-time PCR array, and western blot were performed to explore possible signaling pathways involved in treatment of LF with hPMSCs. Results hPMSC treatment notably alleviates experimental hepatic fibrosis, restores liver function, and inhibits inflammation. Furthermore, the therapeutic effect of hPMSCs against mild-to-moderate LF was significantly greater than against severe LF. In vitro, we observed that the hPMSCs as well as the secretomes of hPMSCs were able to decrease the activation of HSCs. Mechanistic dissection studies showed that hPMSC treatment downregulated the expression of fibrosis-related genes, and this was accompanied by the upregulation of Caveolin-1 (Cav1) (p < 0.001). This suggested that the amelioration of LF occurred partly due to the restoration of Cav1 expression in activated HSCs. Upregulation of Cav1 can inhibit the TGF-β/Smad signaling pathway, mainly by reducing Smad2 phosphorylation, resulting in the inhibition of activated HSCs, whereas this effect could be abated if Cav1 was silenced in advance by siRNAs. Conclusions Our findings suggest that hPMSCs could provide multifaceted therapeutic benefits for the treatment of LF, and the TGF-β/Cav1 pathway might act as a therapeutic target for hPMSCs in the treatment of LF.

scholarly journals Human Menstrual Blood-Derived Stem Cells Ameliorate Liver Fibrosis in Mice by Targeting Hepatic Stellate Cells via Paracrine Mediators

2016 ◽  
Vol 6 (1) ◽  
pp. 272-284 ◽  
Author(s):  
Lijun Chen ◽  
Chunfeng Zhang ◽  
Lu Chen ◽  
Xiaojun Wang ◽  
Bingyu Xiang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Liver Fibrosis ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Menstrual Blood

Human bone marrow mesenchymal stem cells suppress the proliferation of hepatic stellate cells by inhibiting the ubiquitination of p27

2017 ◽  
Vol 95 (6) ◽  
pp. 628-633 ◽  
Author(s):  
Liang Wang ◽  
Guang Bai ◽  
Fei Chen
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Cell Cycle Arrest ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Cycle Arrest ◽  
Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BMSCs) have considerable therapeutic potential for the treatment of end-stage liver disease. Previous studies have demonstrated that BMSCs secrete growth factors and cytokines that inactivate hepatic stellate cells (HSCs), which inhibited the progression of hepatic fibrosis. The aim of this study was to determine the mechanism by which BMSCs suppress the function of HSCs in fibrosis. Our results showed that co-culture of BMSCs and HSCs induced cell cycle arrest at the G10/G1 phase and cell apoptosis of HSCs, which finally inhibited the cell proliferation of HSCs. Consistent with the cell cycle arrest, co-culture of BMSCs and HSCs increased the abundance of the cell cycle protein p27. Mechanistically, we further uncovered that following the co-culture with BMSCs, the expression level of the E3 ligase S-phase kinase-associated protein 2 (SKP2) that is responsible for the ubiquitination of p27 was decreased, which attenuated the ubiquitination of p27 and increased the stability of p27 in HSCs. Collectively, our results indicated the potential involvement of the SKP2–p27 axis for the inhibitory effect of BSMCs on the cell proliferation of HSCs.

Extracellular matrix deposition of bone marrow stroma enhanced by macromolecular crowding

Biomaterials ◽  
2015 ◽  
Vol 73 ◽  
pp. 60-69 ◽  
Author(s):  
Marina C. Prewitz ◽  
Aline Stißel ◽  
Jens Friedrichs ◽  
Nicole Träber ◽  
Steffen Vogler ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Macromolecular Crowding ◽  
Bone Marrow Stroma ◽  
Matrix Deposition ◽  
Marrow Stroma ◽  
Extracellular Matrix Deposition

scholarly journals Bone marrow stem cells-derived extracellular matrix is a promising material

Oncotarget ◽  
2017 ◽  
Vol 8 (58) ◽  
pp. 98336-98347 ◽  
Author(s):  
Xiaoyan Wang ◽  
Guanghua Chen ◽  
Chao Huang ◽  
Hualei Tu ◽  
Jilong Zou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Bone Marrow Stem Cells ◽  
Promising Material
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