scholarly journals SITE-SPECIFIC CHARACTERIZATION OF P450CAM SUBSTRATE RECOGNITION VIA 2D IR SPECTROSCOPY

2020 ◽  
Author(s):  
Sashary Ramos ◽  
Megan Thielges
Keyword(s):  
Ir Spectroscopy ◽  
Substrate Recognition ◽  
Site Specific ◽  
2D Ir

scholarly journals Site-specific 2D IR spectroscopy: a general approach for the characterization of protein dynamics with high spatial and temporal resolution

2019 ◽  
Vol 21 (2) ◽  
pp. 780-788 ◽  
Author(s):  
Sashary Ramos ◽  
Rachel E. Horness ◽  
Jessica A. Collins ◽  
David Haak ◽  
Megan C. Thielges
Keyword(s):  
Ir Spectroscopy ◽  
Spatial Heterogeneity ◽  
Protein Dynamics ◽  
Temporal Resolution ◽  
Protein Structures ◽  
Side Chains ◽  
Conformational Heterogeneity ◽  
Site Specific ◽  
2D Ir

The conformational heterogeneity and dynamics of protein side chains contribute to function, but investigating exactly how is hindered by experimental challenges arising from the fast timescales involved and the spatial heterogeneity of protein structures.

scholarly journals Heterogeneous and Highly Dynamic Interface in Plastocyanin–Cytochrome f Complex Revealed by Site-Specific 2D-IR Spectroscopy

2019 ◽  
Vol 123 (9) ◽  
pp. 2114-2122 ◽  
Author(s):  
Sashary Ramos ◽  
Amanda L. Le Sueur ◽  
Rachel E. Horness ◽  
Jonathan T. Specker ◽  
Jessica A. Collins ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Cytochrome F ◽  
Site Specific ◽  
2D Ir ◽  
Dynamic Interface

Site-Specific Difference 2D-IR Spectroscopy of Bacteriorhodopsin

2009 ◽  
Vol 113 (18) ◽  
pp. 6520-6527 ◽  
Author(s):  
Esben Ravn Andresen ◽  
Peter Hamm
Keyword(s):  
Ir Spectroscopy ◽  
Specific Difference ◽  
Site Specific ◽  
2D Ir

Protein Dynamics by Two-Dimensional Infrared Spectroscopy

2021 ◽  
Vol 14 (1) ◽  
pp. 299-321
Author(s):  
Goran W. Tumbic ◽  
Md Yeathad Hossan ◽  
Megan C. Thielges
Keyword(s):  
Infrared Spectroscopy ◽  
Ir Spectroscopy ◽  
Protein Dynamics ◽  
Two Dimensional ◽  
Spatial And Temporal Scales ◽  
2D Ir ◽  
Functional Dynamics ◽  
Powerful Approach ◽  
Two Dimensional Infrared Spectroscopy

Proteins function as ensembles of interconverting structures. The motions span from picosecond bond rotations to millisecond and longer subunit displacements. Characterization of functional dynamics on all spatial and temporal scales remains challenging experimentally. Two-dimensional infrared spectroscopy (2D IR) is maturing as a powerful approach for investigating proteins and their dynamics. We outline the advantages of IR spectroscopy, describe 2D IR and the information it provides, and introduce vibrational groups for protein analysis. We highlight example studies that illustrate the power and versatility of 2D IR for characterizing protein dynamics and conclude with a brief discussion of the outlook for biomolecular 2D IR.

scholarly journals FTIR and Femtosecond 2D-IR Spectroscopy of Azidohomoalanine-Labeled PDZ3 from PSD-95: Site-Specific Probing of Ultrafast Dynamics and Electrostatics in Proteins

2013 ◽  
Vol 104 (2) ◽  
pp. 29a ◽  
Author(s):  
Henrike M. Mueller-Werkmeister ◽  
Katharina Eberl ◽  
Martin Essig ◽  
Jens Bredenbeck
Keyword(s):  
Ir Spectroscopy ◽  
Ultrafast Dynamics ◽  
Site Specific ◽  
2D Ir

Gelatin and Glycerine-Based Bioadhesive Vaginal Hydrogel

2020 ◽  
Vol 17 (4) ◽  
pp. 303-311
Author(s):  
Roberta Cassano ◽  
Federica Curcio ◽  
Delia Mandracchia ◽  
Adriana Trapani ◽  
Sonia Trombino
Keyword(s):  
Differential Scanning Calorimetry ◽  
Inflammatory Process ◽  
Vaginal Mucosa ◽  
Vaginal Infection ◽  
Scanning Calorimetry ◽  
Time Intervals ◽  
Site Specific ◽  
Release Studies ◽  
Hiv Seropositive

Aim: The work’s aim was the preparation and characterization of a hydrogel based on gelatin and glycerine, useful for site-specific release of benzydamine, an anti-inflammatory drug, able to attenuate the inflammatory process typical of the vaginal infection. Objective: The obtained hydrogel has been characterized by Electronic Scanning Microscopy (SEM) and Differential Scanning Calorimetry (DSC). In addition, due to the precursor properties, the hydrogel exhibits a relevant mucoadhesive activity. Methods: The swelling degree was evaluated at two different pHs and at defined time intervals. In particular, phosphate buffers were used at pH 6.6, in order to mimic the typical conditions of infectious diseases at the vaginal level, particularly for HIV-seropositive pregnant women, and pH 4.6, to simulate the physiological environment. Results: The obtained results revealed that the hydrogel swells up well at both pHs. Conclusion: Release studies conducted at both pathological and physiological pHs have shown that benzydamine is released at the level of the vaginal mucosa in a slow and gradual manner. These data support the hypothesis of the hydrogel use for the site-specific release of benzydamine in the vaginal mucosa.

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