International Rules for Seed Testing

2015 ◽  
Keyword(s):  
Seed Testing

APLIKASI METODE OVEN SUHU TINGGI TETAP DAN BENIH UTUH DALAM PENGUJIAN KADAR AIR BENIH KELAPA SAWIT (Elaeis guineensis L. Jacq.)

2018 ◽  
Vol 26 (3) ◽  
pp. 153-159
Author(s):  
Mohamad Arif ◽  
Nur Muhammad Akbar Illahi
Keyword(s):  
Elaeis Guineensis ◽  
Seed Testing

Kadar air (KA) benih merupakan salah satu faktor penting yang menentukan tingkat viabilitas selama penyimpanan maupun pengecambahan benih. Oleh karena itu, kemampuan untuk menduga KA benih dengan tepat merupakan kebutuhan dasar bagi produsen kecambah. Bagi benih-benih berukuran besar seperti benih kelapa sawit, International Seed Testing Association (ISTA) mensyaratkan penggunaan oven suhu tinggi dan suhu rendah serta penerapan pemecahan benih untuk penentuan KA yang lebih tepat, sedangkan produsen menggunakan benih utuh untuk proses penentuan parameter tersebut. Hasil percobaan memperlihatkan bahwa benih-benih yang diuji dengan oven suhu rendah konstan memberikan nilai KA yang lebih tinggi dibanding KA yang diperlihatkan oleh metode oven suhu tinggi. Selain itu, benih yang dianalisis secara utuh memberikan nilai KA yang tidak berbeda nyata dengan KA benih yang dianalisis dengan metode pemecahan benih. Berdasar komponen penyusun, inti benih memiliki KA yang secara nyata lebih tinggi dibanding KA pada cangkang, baik menggunakan metode oven suhu rendah ataupun suhu tinggi. Percobaan lebih lanjut dibutuhkan mengingat luasnya keragaman ketebalan cangkang benih kelapa sawit.

A needle in a seedstack: an improved method for detection of rare alleles in bulk seed testing through KASP

2021 ◽  
Author(s):  
Anthony Brusa ◽  
Eric L Patterson ◽  
Todd A Gaines ◽  
Kevin Dorn ◽  
Philip Westra ◽  
...  
Keyword(s):  
Improved Method ◽  
Seed Testing ◽  
Rare Alleles

scholarly journals Use of molecular marker techniques in seed testing by Brazilian seed companies

1998 ◽  
Vol 55 (spe) ◽  
pp. 79-82 ◽  
Author(s):  
P.T. Della Vecchia ◽  
C.A.R. da Silva ◽  
P. Terenciano-Sobrinho
Keyword(s):  
Molecular Marker ◽  
New Technologies ◽  
Seed Quality ◽  
Genetic Purity ◽  
Process Step ◽  
Seed Testing ◽  
Routine Basis ◽  
Seed Market ◽  
Key Issues ◽  
High Quality Seed

Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD, microsatelites, AFLP) for genetic purity determinations in seed testing. As far as we know, no Brazilian seed company is using, on regular basis, RAPD or other molecular marker techniques in the determination of genetic purity in seed testing. Most of these are using morphological or physiological traits expressed by seed, seedling or mature plant and/or electrophoresis of seed or seedling proteins/isoenzymes for that purpose. Main reasons for that are: DNA molecular marker techniques are relatively new; lack of specialized personnel to run DNA molecular marker assays on routine basis; higher cost/sample when compared to proteins/isoenzymes electrophoresis.

scholarly journals Effect of colour and size grading of China aster (Callistephus chinensis Nees) seeds on their germination

2012 ◽  
Vol 62 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Agnieszka Rosińska ◽  
Grzegorz Rosiński ◽  
Roman Hołubowicz
Keyword(s):  
Seed Testing ◽  
Royal Horticultural Society ◽  
China Aster ◽  
Colour Chart ◽  
Size Grading ◽  
Better Than

Seeds of 3 commercial China aster (<i>Callistephus chinensis</i> Nees) lots were divided by hand into 3 grades with different colours: dark brown, brown and light brown, and 2 grades with a different size: length below 3.9 mm (small) and above 3.9 mm (large). The colour grading was done based on the Royal Horticultural Society Colour Chart and size grading was done by hand for each seed. Then, seeds were routinely germinated based on the International Seed Testing Association (ISTA) rules. The size of seeds had no effect on their germination. The dark brown seeds germinated better than the light brown ones. Removing light brown seeds from the China aster seed lot improved their germination.

Seed Testing

1999 ◽  
pp. 296-325
Author(s):  
Larry O. Copeland ◽  
Miller B. McDonald
Keyword(s):  
Seed Testing

Routine Estimation of Captan on Individual Sorghum Seeds

1971 ◽  
Vol 54 (5) ◽  
pp. 1110-1112
Author(s):  
Paul W Simon ◽  
Martin M Kulik
Keyword(s):  
Seed Weight ◽  
Seed Treatment ◽  
Colorimetric Method ◽  
Color Intensity ◽  
Average Amount ◽  
Testing Laboratories ◽  
Seed Testing ◽  
Definition Of ◽  
Theoretical Amount

Abstract A colorimetric method to delect captan was modified to permit rapid estimation of this fungicide on individual sorghum seeds. The procedure involves extraction of captan with benzene, removal of interfering dyes with Florisil, reaction with a resorcinol solution, and determination of color intensity with a colorimeter. One hundred seeds from each of 11 commercially treated samples were tested. The seeds numbered from 116,000 to 161,000/ lb. The average amount per sample of captan removed from seeds varied from 10.6 to 28.9 μg/seed and from 0.307 to 1.032 μg/mg seed weight. The theoretical amount of captan should have been 0.975 to 1.400 μg/mg seed weight, according to the container labels. The results indicate the need for closer control of seed treatment and for better definition of amounts of treatment required for adequate seed protection. The method presented now makes it feasible for seed testing laboratories to routinely determine amounts of captan on individual seeds. One technician could probably analyze up to 600 seeds daily, using equipment of larger capacity than the prototypes described in this paper.

EFFECT OF DIFFERENT DAMAGE FACTORS ON SOYBEAN SEED QUALITY

2001 ◽  
Vol 49 (2) ◽  
pp. 133-139 ◽  
Author(s):  
G. A. Lori ◽  
M. N. Sisterna ◽  
M. C. Rollán ◽  
R. A. Barreyro
Keyword(s):  
Buenos Aires ◽  
Seed Quality ◽  
Soybean Seed ◽  
Pathogenic Fungi ◽  
Poor Quality ◽  
Germination Capacity ◽  
Sample Analysis ◽  
Fungal Contamination ◽  
Anatomical Features ◽  
Seed Testing

Poor quality in soybean seed can be due to physiological, pathological or mechanical causes. Seed morphological and anatomical features also make soybean more susceptible to damage factors than other plant species. The purpose of this paper was to evaluate the effects of the different damage factors upon soybean seed quality and its longevity during storage. In two trials, carried out in La Plata (Prov. Buenos Aires, Argentina), soybean seed samples from 7 pre-trading lines, obtained in two consecutive crops, were analysed. Assessments were performed on day 40 and day 160 after harvesting for the first trial and on day 50 and day 150 after harvest in the second. The method employed was the blotter test, following the International Seed Testing Association (ISTA) rules. The parameters evaluated were: damaged cotyledons, rotten seed, fungal contamination and germination capacity. The damage factors had different influences on seed quality. The presence of pathogenic fungi did not necessarily mean low germination capacity. Their effect depended on the degree of infection, the presence of seed-borne pathogens and the time of sample analysis. Rotten seeds and damaged cotyledons caused by moisture had a striking influence on seed quality and preservation during storage.

scholarly journals Laboratory germination testing of the sweet chestnut (Castanea sativa Mill.) according to ISTA rules

2019 ◽  
Vol 143 (9-10) ◽  
pp. 469-477
Author(s):  
Damird Drvodelić ◽  
Igor Poljak ◽  
Marilena Idžojtić ◽  
Ivana Zegnal ◽  
Katarina Tumpa ◽  
...  
Keyword(s):  
Castanea Sativa ◽  
Sweet Chestnut ◽  
Seed Testing ◽  
Castanea Sativa Mill

U radu su prikazani rezultati ispitivanja laboratorijske klijavosti i morfoloških značajki klijanaca europskoga pitomoga kestena (Castanea sativa Mill.). Uzorci za istraživanja sakupljeni su u submediteranskom području Hrvatske, a radni uzorak za ispitivanje klijavosti iznosio je 8×25 sjemenki. Sjeme je do ispitivanja klijavosti čuvano tri mjeseca u hladnjaku na temperaturi od 3 °C. Postotak laboratorijske klijavosti utvrđen je prema postotku pravilnih klijanaca koji su normalno proklijali nakon 27 dana ispitivanja. Ispitivanje je provedeno u laboratoriju prema uvjetima koje propisuje ISTA (International Seed Testing Association). Izrađen je katalog sa slikama i opisom nepravilnih klijanaca. Na osnovi definiranih kodova nepravilnih klijanaca moguće je preventivno djelovati kako bi se reducirale nepoželjne jedinke. Rezultati ovog istraživanja od velike su koristi za razumijevanje složenoga procesa klijanja sjemena, kako u laboratoriju, tako i šumskim rasadnicima.

Sign in / Sign up

Export Citation Format

Share Document