Chapter 6: The topographical tetrazolium test

2022 ◽  
Vol 2022 (1) ◽  
pp. i-6-26
Keyword(s):  
Tetrazolium Test

Suitability of forms of preparing seeds of Dalbergia miscolobium Benth for exposure to the tetrazolium test / Adequação das formas de preparação de sementes de Dalbergia miscolobium Benth para exposição ao teste de tetrazolium

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Juliana Martins de Mesquita Matos ◽  
Rosana De Carvalho Cristo Martins ◽  
Valéria Regina Bellotto ◽  
Lilian Gomes da Silva Rocha ◽  
Eloiza Aparecida Barbosa ◽  
...  
Keyword(s):  
Endangered Species ◽  
Filter Paper ◽  
Federal District ◽  
Complete Removal ◽  
Sensu Stricto ◽  
The Other ◽  
Distrito Federal ◽  
Viable Seeds ◽  
Range Test ◽  
Tetrazolium Test

Dalbergia miscolobium or Jacarandá do Cerrado is a species of legume in the Fabaceae family. It occurs in the sensu stricto Cerrado and in the dystrophic cerradão. It shows potential for landscaping and for recovering damaged areas. It is an endangered species and therefore is protected by the law that prevents cut in areas of the Federal District (Decree No. 14.783/93). The purpose of this study was to determine the best procedure to prepare seeds of Dalbergia miscolobium to assess viability in the tetrazolium test. We carried out the following treatments: i) hydration on filter paper at 25 ° C, ii) hydration on filter paper at 25 ° C followed by a cut in the tegument and iii) hydration on filter paper at 25 ° C followed bya complete removal of the tegument. The results were analyzed using analysis of variance and the Tukey range test. The analyzes showed that the best procedure to prepare seeds of Dalbergia miscolobium is the treatment in which there is a hydration followed by the complete removal of the integument. Where 78% of the seeds showed uniform staining, indicating that the seeds analyzed are of good quality. The other treatments, hydration and hydration followed by cutting, showed respectively 35% and 41% of viable seeds. RESUMO A Dalbergia miscolobium ou Jacarandá do Cerrado é uma espécie de leguminosa da família Fabaceae. Ocorre no sentido stricto Cerrado e no cerradão distrófico. Possui potencial para paisagismo e para recuperar áreas degradadas. É uma espécie ameaçada de extinção e, portanto, está protegida pela lei que previne o corte em áreas do Distrito Federal (Decreto 14.783 / 93). O objetivo deste estudo foi determinar o melhor procedimento de prepararação das sementes de Dalbergia miscolobium para serem submetidas à análise de viabilidade pelo teste de tetrazólio. Foram realizados os seguintes tratamentos: i) hidratação em papel de filtro a 25 ° C, ii) hidratação em papel de filtro a 25 ° C seguida de um corte no tegumento e iii) hidratação em papel de filtro a 25 ° C seguido de remoção completa do tegumento. Os resultados foram analisados utilizando-se a análise de variância e o teste de médias de Tukey. As análises mostraram que o melhor procedimento para preparar sementes de Dalbergia miscolobium é o tratamento em que há uma hidratação seguida pela remoção completa do tegumento, onde 78% das sementes apresentaram coloração uniforme, indicando que as sementes analisadas são de boa qualidade. Os demais tratamentos, hidratação e hidratação seguida de corte, mostraram respectivamente 35% e 41% de sementes viáveis.

The Nitroblue Tetrazolium Test in Tuberculosis

1974 ◽  
Vol 62 (3) ◽  
pp. 431-434
Author(s):  
Sally E. Ryden ◽  
Eugene M. Silverman
Keyword(s):  
Nitroblue Tetrazolium ◽  
Nitroblue Tetrazolium Test ◽  
Tetrazolium Test

scholarly journals PHYSIOLOGICAL POTENTIAL AND CONSERVATION OF MURUCI (Byrsonima crassifolia) SEEDS

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
CAMILA RIBEIRO DE SOUZA GRZYBOWSKI ◽  
WALNICE MARIA OLIVEIRA DO NASCIMENTO ◽  
ROSEMEIRE CARVALHO DA SILVA ◽  
ELISA SERRA NEGRA VIEIRA ◽  
MARISTELA PANOBIANCO
Keyword(s):  
Block Design ◽  
Rapid Assessment ◽  
Germplasm Collection ◽  
Germination Test ◽  
Byrsonima Crassifolia ◽  
Randomized Block Design ◽  
Relative Air Humidity ◽  
Different Temperatures ◽  
Mother Plants ◽  
Tetrazolium Test

ABSTRACT The goal of the present study is to assess the physiological potential of Byrsonima crassifoliaseeds and analyze the procedures of the germination and tetrazolium tests, as well as the conservation of such seeds by testing different combinations of packaging and environment. The experiment used pyrenes of the clone Açu, collected from mother plants in the germplasm collection of muruci plants of Embrapa Amazonia Oriental, in Belém, Brazil. Different temperatures were tested in the germination test (25, 30, 35 and 20 - 30 ºC). The best methodology and assessment dates were determined for the test, and the seedlings of the species were characterized. Viability was assessed with the tetrazolium test, with combinations of hydration methods (by immersion and between paper towels), staining periods (3 and 4 h) and concentrations of tetrazolium solution (0.5, 0.7 and 1.0%). Storage of muruci pyrenes was evaluated at three, six and twelve months, in polyethylene and Kraft paper bags, under dry chamber and refrigerator conditions. The study used a randomized block design with four replicates. The data underwent analysis of variance. In conclusion, the physiological potential of seeds of B. crassifoliacan be assessed by the germination test conducted in sand, with alternating temperatures of 20-30 ºC, or with the use of the tetrazolium test (with staining for three hours in the tetrazolium solution at 1.0%) as a rapid assessment of viability. Muruci pyrenes can be kept in polythene or paper Kraft bags for up to 12 months, in a dry chamber at 16 ºC and with relative air humidity of 50 to 60%.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

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