scholarly journals Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli

2018 ◽  
Vol 14 (6) ◽  
Author(s):  
Manuel Campos ◽  
Sander K Govers ◽  
Irnov Irnov ◽  
Genevieve S Dobihal ◽  
François Cornet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Cycle ◽  
Cell Morphogenesis ◽  
Phenotypic Analysis

scholarly journals Genome-wide phenotypic analysis of growth, cell morphogenesis and cell cycle events in Escherichia coli.

2017 ◽  
Author(s):  
Manuel Campos ◽  
Sander K Govers ◽  
Irnov Irnov ◽  
Genevieve S Dobihal ◽  
Francois Cornet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Cycle ◽  
Growth Rate ◽  
Cell Division ◽  
Cell Size ◽  
Gene Knockout ◽  
Single Gene ◽  
Cell Morphogenesis ◽  
Phenotypic Analysis ◽  
New Genes

Cell size, cell growth and the cell cycle are necessarily intertwined to achieve robust bacterial replication. Yet, a comprehensive and integrated view of these fundamental processes is lacking. Here, we describe an image-based quantitative screen of the single-gene knockout collection of Escherichia coli, and identify many new genes involved in cell morphogenesis, population growth, nucleoid (bulk chromosome) dynamics and cell division. Functional analyses, together with high-dimensional classification, unveil new associations of morphological and cell cycle phenotypes with specific functions and pathways. Additionally, correlation analysis across ~4,000 genetic perturbations shows that growth rate is surprisingly not predictive of cell size. Growth rate was also uncorrelated with the relative timings of nucleoid separation and cell constriction. Rather, our analysis identifies scaling relationships between cell size and nucleoid size and between nucleoid size and the relative timings of nucleoid separation and cell division. These connections suggest that the nucleoid links cell morphogenesis to the cell cycle.

scholarly journals HU content and dynamics in Escherichia coli during the cell cycle and at different growth rates

2017 ◽  
Vol 364 (19) ◽  
Author(s):  
Anteneh Hailu Abebe ◽  
Alexander Aranovich ◽  
Itzhak Fishov
Keyword(s):  
Escherichia Coli ◽  
Cell Cycle ◽  
Growth Rates

scholarly journals Lipid synthesis during the Escherichia coli cell cycle.

1981 ◽  
Vol 145 (1) ◽  
pp. 472-478 ◽  
Author(s):  
C E Carty ◽  
L O Ingram
Keyword(s):  
Escherichia Coli ◽  
Cell Cycle ◽  
Lipid Synthesis ◽  
Coli Cell

Genomic and phenotypic analysis of heat and sanitizer resistance in Escherichia coli from beef in relation to the locus of heat resistance

2021 ◽  
Author(s):  
Xianqin Yang ◽  
Frances Tran ◽  
Peipei Zhang ◽  
Hui Wang
Keyword(s):  
Escherichia Coli ◽  
Heat Resistance ◽  
Phylogenetic Relationships ◽  
Core Genome ◽  
Elevated Temperatures ◽  
Resistant Bacteria ◽  
Phenotypic Analysis ◽  
Decimal Reduction Time ◽  
E Coli ◽  
Clade 1

The locus of heat resistance (LHR) can confer heat resistance to Escherichia coli to various extents. This study investigated the phylogenetic relationships, and genomic and phenotypic characteristics of E. coli with or without LHR recovered from beef by direct plating or from enrichment broth at 42°C. LHR-positive E. coli isolates (n=24) were whole genome-sequenced by short- and long-reads. LHR-negative isolates (n=18) from equivalent sources as LHR-positive isolates were short-read sequenced. All isolates were assessed for decimal reduction time at 60°C ( D 60°C ) and susceptibility to E-SAN and Perox-E. Selected isolates were evaluated for growth at 42°C. The LHR-positive and negative isolates were well separated on the core genome tree, with 22/24 of the positive isolates clustering into three clades. Isolates within clade 1 and 2, despite their different D 60°C values, were clonal, as determined by subtyping (MLST, core genome MLST, and serotyping). Isolates within each clade are of one serotype. The LHR-negative isolates were genetically diverse. The LHR-positive isolates had a larger (p<0.001) median genome size by 0.3 Mbp (5.0 vs 4.7 Mbp), and overrepresentation of genes in plasmid maintenance, stress response and cryptic prophages, but underrepresentation of genes involved in epithelial attachment and virulence. All LHR-positive isolates harbored a chromosomal copy of LHR, and all clade 2 isolates had an additional partial copy of LHR on conjugative plasmids. The growth rates at 42°C were 0.71±0.02 and 0.65±0.02 logOD h −1 for LHR-positive and negative isolates. No meaningful difference in sanitizer susceptibility was noted between LHR-positive and negative isolates. Importance Resistant bacteria are serious food safety and public health concerns. Heat resistance conferred by the LHR varies largely among different strains. The findings in this study show that genomic background and composition of LHR, in addition to the presence of LHR, play an important role in the degree of heat resistance in E. coli , and that strains with certain genetic background are more likely to acquire and maintain the LHR. Also, caution should be exercised when recovering E. coli at elevated temperatures as the presence of LHR may confer growth advantages to some strains. Interestingly, the LHR harboring strains seem to have evolved further from their primary animal host to adapt to their secondary habitat, as reflected by fewer genes in virulence and epithelial attachment. The phylogenetic relationships among the isolates point towards multiple mechanisms for acquiring LHR, likely prior to their deposition on meat.

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