scholarly journals Cytometry‐based single‐cell analysis of intact epithelial signaling reveals MAPK activation divergent from TNF ‐α‐induced apoptosis in vivo

2016 ◽  
Vol 12 (8) ◽  
pp. 881
Author(s):  
Alan J Simmons ◽  
Amrita Banerjee ◽  
Eliot T McKinley ◽  
Cherie’ R Scurrah ◽  
Charles A Herring ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Mapk Activation ◽  
Tnf Α ◽  
Induced Apoptosis

scholarly journals P-165 Cytometry-Based Single Cell Analysis of Intact Epithelial Signaling Reveals MAPK Activation Divergent from TNF-α-Induced Apoptosis in Vivo

2016 ◽  
Vol 22 ◽  
pp. S59-S60
Author(s):  
Alan Simmons ◽  
Amrita Banerjee ◽  
Eliot McKinley ◽  
Cherieʼ Scurrah ◽  
Jeffrey Franklin ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Mapk Activation ◽  
Tnf Α ◽  
Induced Apoptosis

scholarly journals Cytometry‐based single‐cell analysis of intact epithelial signaling reveals MAPK activation divergent from TNF ‐α‐induced apoptosis in vivo

2015 ◽  
Vol 11 (10) ◽  
pp. 835 ◽  
Author(s):  
Alan J Simmons ◽  
Amrita Banerjee ◽  
Eliot T McKinley ◽  
Cherie' R Scurrah ◽  
Charles A Herring ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Mapk Activation ◽  
Tnf Α ◽  
Induced Apoptosis

scholarly journals Single Cell Analysis Reveals That IL-4 Receptor/Stat6 Signaling Is Not Required for the In Vivo or In Vitro Development of CD4+Lymphocytes with a Th2 Cytokine Profile

2000 ◽  
Vol 164 (6) ◽  
pp. 3047-3055 ◽  
Author(s):  
Dragana Jankovic ◽  
Marika C. Kullberg ◽  
Nancy Noben-Trauth ◽  
Patricia Caspar ◽  
William E. Paul ◽  
...  
Keyword(s):  
Single Cell ◽  
Single Cell Analysis ◽  
Cytokine Profile ◽  
Cell Analysis ◽  
In Vitro Development ◽  
Th2 Cytokine ◽  
Vitro Development ◽  
Cd4 Lymphocytes

scholarly journals Single-cell analysis of signal transduction in CD4 T cells stimulated by antigen in vivo

2001 ◽  
Vol 98 (19) ◽  
pp. 10805-10810 ◽  
Author(s):  
T. Zell ◽  
A. Khoruts ◽  
E. Ingulli ◽  
J. L. Bonnevier ◽  
D. L. Mueller ◽  
...  
Keyword(s):  
Signal Transduction ◽  
T Cells ◽  
Single Cell ◽  
Cd4 T Cells ◽  
Single Cell Analysis ◽  
Cell Analysis

scholarly journals Single Cell Sequencing Reveals Early PGC-like Intermediates During Mouse Primed to Naïve Transition

2021 ◽  
Author(s):  
Jing Liu ◽  
Shengyong Yu ◽  
Chunhua Zhou ◽  
Jiangping He ◽  
Xingnan Huang ◽  
...  
Keyword(s):  
Germ Cell ◽  
Single Cell ◽  
Cell Fate ◽  
Single Cell Analysis ◽  
Primordial Germ Cell ◽  
Model System ◽  
Cell Analysis ◽  
Rna Seq ◽  
Single Cell Sequencing

Abstract Single cell analysis provides clarity unattainable with bulk approaches. Here we apply single cell RNA-seq to a newly established BMP4 induced mouse primed to naive transition (Bi-PNT) system and show that the reset is not a direct reversal of cell fate but through developmental intermediates. We first show that mEpiSCs bifurcate into c-Kit+ naïve and c-Kit- placenta-like cells, among which, the naive branch undergoes further transition through a primordial germ cell-like cells (PGCLCs) intermediate capable of spermatogenesis in vivo. Indeed, deficiency of Prdm1/Blimp1, the key regulator for PGC specification, blocks the induction of PGCLCs and naïve cells. Instead, Gata2 knockout arrests placenta-like fate, but facilitates the generation of PGCLCs. Our results not only reveal a newly cell fate dynamics between primed and naive states at single-cell resolution, but also provide a model system to explore mechanisms involved in regaining germline competence from primed pluripotency.

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