A retained intron in the 3′‐
            UTR
            of
            Calm3
            mRNA
            mediates its Staufen2‐ and activity‐dependent localization to neuronal dendrites

Tejaswini Sharangdhar; Yoichiro Sugimoto; Jacqueline Heraud‐Farlow; Sandra M Fernández‐Moya; Janina Ehses; Igor Ruiz de los Mozos; Jernej Ule; Michael A Kiebler

doi:10.15252/embr.201744334

A retained intron in the 3′‐ UTR of Calm3 mRNA mediates its Staufen2‐ and activity‐dependent localization to neuronal dendrites

EMBO Reports ◽

10.15252/embr.201744334 ◽

2017 ◽

Vol 18 (10) ◽

pp. 1762-1774 ◽

Cited By ~ 17

Author(s):

Tejaswini Sharangdhar ◽

Yoichiro Sugimoto ◽

Jacqueline Heraud‐Farlow ◽

Sandra M Fernández‐Moya ◽

Janina Ehses ◽

...

Keyword(s):

Neuronal Dendrites ◽

Retained Intron ◽

Activity Dependent

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Activity-dependent spatially localized miRNA maturation in neuronal dendrites

Science ◽

10.1126/science.aaf8995 ◽

2017 ◽

Vol 355 (6325) ◽

pp. 634-637 ◽

Cited By ~ 92

Author(s):

Sivakumar Sambandan ◽

Güney Akbalik ◽

Lisa Kochen ◽

Jennifer Rinne ◽

Josefine Kahlstatt ◽

...

Keyword(s):

Neuronal Dendrites ◽

Activity Dependent

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Neuronal cotransport of glycine receptor and the scaffold protein gephyrin

The Journal of Cell Biology ◽

10.1083/jcb.200506066 ◽

2006 ◽

Vol 172 (3) ◽

pp. 441-451 ◽

Cited By ~ 86

Author(s):

Christoph Maas ◽

Nadia Tagnaouti ◽

Sven Loebrich ◽

Bardo Behrend ◽

Corinna Lappe-Siefke ◽

...

Keyword(s):

Plasma Membranes ◽

Glycine Receptor ◽

Scaffold Protein ◽

Inhibitory Synapses ◽

Motor Complex ◽

Neuronal Dendrites ◽

Dynein Motor ◽

Cytoskeletal Elements ◽

Transport Complex ◽

Activity Dependent

The dynamics of postsynaptic receptor scaffold formation and remodeling at inhibitory synapses remain largely unknown. Gephyrin, which is a multimeric scaffold protein, interacts with cytoskeletal elements and stabilizes glycine receptors (GlyRs) and individual subtypes of γ-aminobutyric acid A receptors at inhibitory postsynaptic sites. We report intracellular mobility of gephyrin transports packets over time. Gephyrin units enter and exit active synapses within several minutes. In addition to previous reports of GlyR–gephyrin interactions at plasma membranes, we show cosedimentation and coimmunoprecipitation of both proteins from vesicular fractions. Moreover, GlyR and gephyrin are cotransported within neuronal dendrites and further coimmunoprecipitate and colocalize with the dynein motor complex. As a result, the blockade of dynein function or dynein–gephyrin interaction, as well as the depolymerization of microtubules, interferes with retrograde gephyrin recruitment. Our data suggest a GlyR–gephyrin–dynein transport complex and support the concept that gephyrin–motor interactions contribute to the dynamic and activity-dependent rearrangement of postsynaptic GlyRs, a process thought to underlie the regulation of synaptic strength.

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Antibodies directed against retained intron 4 of the constitutively active CCK-2 receptor bind to a tumour-specific epitope

Gastroenterology ◽

10.1016/s0016-5085(01)82463-x ◽

2001 ◽

Vol 120 (5) ◽

pp. A496-A496 ◽

Cited By ~ 1

Author(s):

P CLARKE ◽

S GRIMES ◽

D MCWILLIAMS ◽

W KLEIE ◽

K KHAN ◽

...

Keyword(s):

Specific Epitope ◽

Retained Intron ◽

Intron 4 ◽

Constitutively Active

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Malfunctional Reorganization in the Developing Limbo-Prefrontal System in Animals: Implications for Human Psychoses?

Zeitschrift für Neuropsychologie ◽

10.1024//1016-264x.12.1.8 ◽

2001 ◽

Vol 12 (1) ◽

pp. 8-14

Author(s):

Gertraud Teuchert-Noodt ◽

Ralf R. Dawirs

Keyword(s):

Prefrontal Cortex ◽

Mental Disorders ◽

Dentate Gyrus ◽

Cognitive Functions ◽

Experimental Approach ◽

Regulatory Mechanisms ◽

Hippocampal Dentate Gyrus ◽

Non Invasive ◽

Invasive Method ◽

Activity Dependent

Abstract: Neuroplasticity research in connection with mental disorders has recently bridged the gap between basic neurobiology and applied neuropsychology. A non-invasive method in the gerbil (Meriones unguiculus) - the restricted versus enriched breading and the systemically applied single methamphetamine dose - offers an experimental approach to investigate psychoses. Acts of intervening affirm an activity dependent malfunctional reorganization in the prefrontal cortex and in the hippocampal dentate gyrus and reveal the dopamine position as being critical for the disruption of interactions between the areas concerned. From the extent of plasticity effects the probability and risk of psycho-cognitive development may be derived. Advance may be expected from insights into regulatory mechanisms of neurogenesis in the hippocampal dentate gyrus which is obviously to meet the necessary requirements to promote psycho-cognitive functions/malfunctions via the limbo-prefrontal circuit.

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