scholarly journals Live‐cell imaging reveals the interplay between transcription factors, nucleosomes, and bursting

2019 ◽  
Vol 38 (12) ◽  
Author(s):  
Benjamin T Donovan ◽  
Anh Huynh ◽  
David A Ball ◽  
Heta P Patel ◽  
Michael G Poirier ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Imaging of native transcription factors and histone phosphorylation at high resolution in live cells

2018 ◽  
Vol 217 (4) ◽  
pp. 1537-1552 ◽  
Author(s):  
Sascha Conic ◽  
Dominique Desplancq ◽  
Alexia Ferrand ◽  
Veronique Fischer ◽  
Vincent Heyer ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Rna Polymerase Ii ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Cell Types ◽  
Fluorescent Labeling ◽  
Live Cell ◽  
Biological Information ◽  
Live Cells

Fluorescent labeling of endogenous proteins for live-cell imaging without exogenous expression of tagged proteins or genetic manipulations has not been routinely possible. We describe a simple versatile antibody-based imaging approach (VANIMA) for the precise localization and tracking of endogenous nuclear factors. Our protocol can be implemented in every laboratory allowing the efficient and nonharmful delivery of organic dye-conjugated antibodies, or antibody fragments, into different metazoan cell types. Live-cell imaging permits following the labeled probes bound to their endogenous targets. By using conventional and super-resolution imaging we show dynamic changes in the distribution of several nuclear transcription factors (i.e., RNA polymerase II or TAF10), and specific phosphorylated histones (γH2AX), upon distinct biological stimuli at the nanometer scale. Hence, considering the large panel of available antibodies and the simplicity of their implementation, VANIMA can be used to uncover novel biological information based on the dynamic behavior of transcription factors or posttranslational modifications in the nucleus of single live cells.

Simple Fluorescence Turn-On Chemosensor for Selective Detection of Ba2+ Ion and Its Live Cell Imaging

2019 ◽  
Vol 91 (15) ◽  
pp. 10095-10101 ◽  
Author(s):  
Palanisamy Ravichandiran ◽  
Sivakumar Allur Subramaniyan ◽  
Antony Paulraj Bella ◽  
Princy Merlin Johnson ◽  
Ae Rhan Kim ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On
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