scholarly journals Hierarchical protein targeting and secretion is controlled by an affinity switch in the type III secretion system of enteropathogenic Escherichia coli

2017 ◽  
Vol 36 (23) ◽  
pp. 3517-3531 ◽  
Author(s):  
Athina G Portaliou ◽  
Konstantinos C Tsolis ◽  
Maria S Loos ◽  
Vassileia Balabanidou ◽  
Josep Rayo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Protein Targeting ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Enteropathogenic Escherichia Coli ◽  
Type Iii

scholarly journals EscA Is a Crucial Component of the Type III Secretion System of Enteropathogenic Escherichia coli

2012 ◽  
Vol 194 (11) ◽  
pp. 2819-2828 ◽  
Author(s):  
N. Sal-Man ◽  
E. Biemans-Oldehinkel ◽  
D. Sharon ◽  
M. A. Croxen ◽  
R. Scholz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Enteropathogenic Escherichia Coli ◽  
Type Iii ◽  
Crucial Component

EscI: a crucial component of the type III secretion system forms the inner rod structure in enteropathogenic Escherichia coli

2012 ◽  
Vol 442 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Neta Sal-Man ◽  
Wanyin Deng ◽  
B. Brett Finlay
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Integral Membrane Protein ◽  
Secretion System ◽  
Host Cells ◽  
Enteropathogenic Escherichia Coli ◽  
Bacterial Membranes ◽  
Type Iii ◽  
Protein Functions

The T3SS (type III secretion system) is a multi-protein complex that plays a central role in the virulence of many Gram-negative bacterial pathogens. This apparatus spans both bacterial membranes and transports virulence factors from the bacterial cytoplasm into eukaryotic host cells. The T3SS exports substrates in a hierarchical and temporal manner. The first secreted substrates are the rod/needle proteins which are incorporated into the T3SS apparatus and are required for the secretion of later substrates, the translocators and effectors. In the present study, we provide evidence that rOrf8/EscI, a poorly characterized locus of enterocyte effacement-encoded protein, functions as the inner rod protein of the T3SS of EPEC (enteropathogenic Escherichia coli). We demonstrate that EscI is essential for type III secretion and is also secreted as an early substrate of the T3SS. We found that EscI interacts with EscU, the integral membrane protein that is linked to substrate specificity switching, implicating EscI in the substrate-switching event. Furthermore, we showed that EscI self-associates and interacts with the outer membrane secretin EscC, further supporting its function as an inner rod protein. Overall, the results of the present study suggest that EscI is the YscI/PrgJ/MxiI homologue in the T3SS of attaching and effacing pathogens.

Function and distribution of EspG2, a type III secretion system effector of enteropathogenic Escherichia coli

2006 ◽  
Vol 8 (8) ◽  
pp. 2220-2227 ◽  
Author(s):  
Katherine Smollett ◽  
Robert K. Shaw ◽  
Junkal Garmendia ◽  
Stuart Knutton ◽  
Gad Frankel
Keyword(s):  
Escherichia Coli ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Enteropathogenic Escherichia Coli ◽  
Type Iii
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