scholarly journals Parental Behavior Controls Incubation Period and Asynchrony of Hatching in Magellanic Penguins: Reply to Demongin, Poisbleau and Eens (2013)

The Condor ◽  
2013 ◽  
Vol 115 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Ginger A. Rebstock ◽  
P. Dee Boersma
Keyword(s):  
Incubation Period ◽  
Parental Behavior ◽  
Magellanic Penguins

scholarly journals Foraging areas of Magellanic penguins Spheniscus magellanicus breeding at San Lorenzo, Argentina, during the incubation period

1995 ◽  
Vol 129 ◽  
pp. 1-6 ◽  
Author(s):  
RP Wilson ◽  
JA Scolaro ◽  
G Peters ◽  
S Laurenti ◽  
M Kierspel ◽  
...  
Keyword(s):  
Incubation Period ◽  
San Lorenzo ◽  
Spheniscus Magellanicus ◽  
Magellanic Penguins

Parental behavior and child distress and pain during pediatric medical procedures: Systematic review and meta-analysis.

2020 ◽  
Vol 39 (7) ◽  
pp. 558-572
Author(s):  
Małgorzata Sobol-Kwapińska ◽  
Marek Sobol ◽  
Ewa Woźnica-Niesobska
Keyword(s):  
Systematic Review ◽  
Parental Behavior ◽  
Meta Analysis ◽  
Medical Procedures ◽  
Child Distress

Effect of Temperature on ADP-Induced Platelet Aggregation

1973 ◽  
Vol 29 (01) ◽  
pp. 183-189
Author(s):  
C. A Praga ◽  
E. M Pogliani
Keyword(s):  
Platelet Aggregation ◽  
Incubation Period ◽  
Room Temperature ◽  
Important Variable ◽  
Practical Significance ◽  
Effect Of Temperature ◽  
Induce Platelet Aggregation ◽  
Cuvette Holder ◽  
Exact Temperature

SummaryTemperature represents a very important variable in ADP-induced platelet aggregation.When low doses of ADP ( < 1 (μM) are used to induce platelet aggregation, the length of the incubation period of PRP in the cuvette holder of the aggregometer, thermostatted at 37° C, is very critical. Samples of the same PRP previously kept at room temperature, were incubated for increasing periods of time in the cuvette of the aggregometer before adding ADP, and a significant decrease of aggregation, proportional to the length of incubation, was observed. Stirring of the PRP during the incubation period made these changes more evident.To measure the exact temperature of the PRP during incubation in the aggre- gometer, a thermocouple device was used. While the temperature of the cuvette holder was stable at 37° C, the PRP temperature itself increased exponentially, taking about ten minutes from the beginning of the incubation to reach the value of 37° C. The above results have a practical significance in the reproducibility of the platelet aggregation test in vitro and acquire particular value when the effect of inhibitors of ADP induced platelet aggregation is studied.Experiments carried out with three anti-aggregating agents (acetyl salicyclic acid, dipyridamole and metergoline) have shown that the incubation conditions which influence both the effect of the drugs on platelets and the ADP breakdown in plasma must be strictly controlled.

Colchicine Uptake and Binding by Human Platelets

1975 ◽  
Vol 34 (03) ◽  
pp. 780-794 ◽  
Author(s):  
Dianne M Kenney ◽  
Francis C Chao ◽  
James L Tullis ◽  
Gail S Conneely
Keyword(s):  
Time Dependence ◽  
Incubation Period ◽  
Binding Sites ◽  
Silicone Oil ◽  
Cold Treatment ◽  
Human Platelets ◽  
Temperature Dependent

SummaryThe uptake and binding of antimitotic alkaloid colchicine has been demonstrated in washed preparations of human platelets. A silicone oil technique was adapted so that both uptake and binding of 14C-colchicine were examined in the same platelet preparations. The time dependence and amount of colchicine taken up and bound by different platelet preparations during a 90 to 120 min incubation period were highly reproducible. Both colchicine uptake and binding by intact platelets, and colchicine binding by preparations of lysed platelets were specific and temperature dependent. Colchicine uptake was slowly reversible. Magnesium and GTP enhanced colchicine binding by lysed platelet preparations but calcium decreased binding.Exposure of platelets to either cold (4° C) or to thrombin, which disrupt platelet microtubules, produced significant increases in colchicine uptake and binding. The thrombin effect was maximal at 37° C and resulted in a greater increase in uptake and binding than that produced by either cold treatment alone or, by cold treatment followed by incubation with thrombin at 37° C. The amount of increase in uptake and binding produced by thrombin was independent of both thrombin (1–5 Units/109 platelets) and colchicine concentrations (1–50 × 10−6M).It is postulated that thrombin may initiate the formation, or make available, colchicine binding sites (microtubule subunits) within platelets.

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