International Journal of Advances in Chemical Engineering and Biological Sciences

First, let me introduce myself. I am the Product Development and Downstream Processing Manager for an SME, ProMetic BioSciences Ltd (PBL) based in Cambridge, UK. I did my undergraduate degree in Biological Sciences at the University of Wolverhampton during which I completed a sandwich placement in the Chemical Engineering department at the University of Birmingham. After I completed my degree I went back to the same group (The Biochemical Recovery Group) at Birmingham to do my PhD.

Download Full-text

Dynamical Scattering in Crystalline Biological Specimens. I. Criteria of Validity for Scattering Approximations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010011458x ◽

1975 ◽

Vol 33 ◽

pp. 192-193

Author(s):

Robert M. Glaeser ◽

Bing K. Jap

Keyword(s):

Biological Sciences ◽

Electron Microscopy ◽

Electron Microscope ◽

Electron Diffraction ◽

Born Approximation ◽

Materials Science ◽

Image Data ◽

Dynamical Effect ◽

Crystallographic Structure ◽

Electron Microscope Image

The dynamical scattering effect, which can be described as the failure of the first Born approximation, is perhaps the most important factor that has prevented the widespread use of electron diffraction intensities for crystallographic structure determination. It would seem to be quite certain that dynamical effects will also interfere with structure analysis based upon electron microscope image data, whenever the dynamical effect seriously perturbs the diffracted wave. While it is normally taken for granted that the dynamical effect must be taken into consideration in materials science applications of electron microscopy, very little attention has been given to this problem in the biological sciences.

Download Full-text

The Application of Ultra-Thin Sectioning Techniques to Non-Biological Samples

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100101402 ◽

1968 ◽

Vol 26 ◽

pp. 332-333

Author(s):

C. F. Oster

Keyword(s):

Biological Sciences ◽

Epoxy Resin ◽

Cross Sections ◽

Biological Samples ◽

Industrial Applications ◽

Photographic Film ◽

Silver Particles ◽

Thin Sectioning ◽

Embedding Medium

Although ultra-thin sectioning techniques are widely used in the biological sciences, their applications are somewhat less popular but very useful in industrial applications. This presentation will review several specific applications where ultra-thin sectioning techniques have proven invaluable.The preparation of samples for sectioning usually involves embedding in an epoxy resin. Araldite 6005 Resin and Hardener are mixed so that the hardness of the embedding medium matches that of the sample to reduce any distortion of the sample during the sectioning process. No dehydration series are needed to prepare our usual samples for embedding, but some types require hardening and staining steps. The embedded samples are sectioned with either a prototype of a Porter-Blum Microtome or an LKB Ultrotome III. Both instruments are equipped with diamond knives.In the study of photographic film, the distribution of the developed silver particles through the layer is important to the image tone and/or scattering power. Also, the morphology of the developed silver is an important factor, and cross sections will show this structure.

Download Full-text