Characterization of Crack Closure

2008 ◽  
pp. 361-361-20 ◽  
Author(s):  
H Sehitoglu
Keyword(s):  
Crack Closure

Characterization of Discontinuous Crack Closure Behavior after the Application of a Single Overload Cycle

2021 ◽  
pp. 103028
Author(s):  
Giancarlo Luis Gómez Gonzáles ◽  
Julián Andrés Ortiz González ◽  
José Luiz de França Freire
Keyword(s):  
Crack Closure ◽  
Single Overload

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

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