Controlled-Strain Testing Procedures

You get what you screen for: on the value of fermentation characterization in high-throughput strain improvements in industrial settings

Journal of Industrial Microbiology & Biotechnology ◽

10.1007/s10295-020-02295-3 ◽

2020 ◽

Vol 47 (11) ◽

pp. 913-927 ◽

Cited By ~ 2

Author(s):

Maren Wehrs ◽

Alexander de Beaumont-Felt ◽

Alexi Goranov ◽

Patrick Harrigan ◽

Stefan de Kok ◽

...

Keyword(s):

High Throughput ◽

Testing Procedures ◽

Strain Design ◽

Design Build ◽

Strain Testing ◽

Depth Study ◽

Improved Performance ◽

Cell Densities ◽

Frequent Sampling ◽

Industrial Context

AbstractWhile design and high-throughput build approaches in biotechnology have increasingly gained attention over the past decade, approaches to test strain performance in high-throughput have received less discussion in the literature. Here, we describe how fermentation characterization can be used to improve the overall efficiency of high-throughput DBTAL (design-build-test-analyze-learn) cycles in an industrial context. Fermentation characterization comprises an in-depth study of strain performance in a bioreactor setting and involves semi-frequent sampling and analytical measurement of substrates, cell densities and viabilities, and (by)products. We describe how fermentation characterization can be used to (1) improve (high-throughput) strain design approaches; (2) enable the development of bench-scale fermentation processes compatible with a wide diversity of strains; and (3) inform the development of high-throughput plate-based strain testing procedures for improved performance at larger scales.

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Elaborative testing procedures [abstract]

PsycEXTRA Dataset ◽

10.1037/e602442011-002 ◽

1981 ◽

Keyword(s):

Testing Procedures

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Automation of Testing Procedures.

American Psychologist ◽

10.1037/h0020548 ◽

1969 ◽

Vol 24 (12) ◽

pp. 1174-1174

Author(s):

Joseph L. Moses

Keyword(s):

Testing Procedures

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Rubber Fracture Characterization Using J-Integral

Tire Science and Technology ◽

10.2346/1.2148797 ◽

1988 ◽

Vol 16 (1) ◽

pp. 44-60 ◽

Cited By ~ 14

Author(s):

C. L. Chow ◽

J. Wang ◽

P. N. Tse

Keyword(s):

Crack Initiation ◽

Carbon Black ◽

Natural Rubber ◽

Fracture Parameter ◽

J Integral ◽

Specimen Length ◽

Fracture Characterization ◽

Premature Failure ◽

Testing Procedures ◽

Material Evaluation

Abstract The use of the J-integral to investigate fracture characterization in a carbon black reinforced natural rubber is described. Three applications to crack initiation are included: two based on the use of a hypothetical zero specimen length and one on conventional testing procedures for metals. While the validity of the zero-length methods is questionable, the conventional method yielded a consistent Jc value of 1.01 N/mm for a typical tire compound. This value was obtained from 24 combinations of varying specimen geometries and pre-crack lengths. The J-integral is revealed as a valid fracture parameter that is applicable not only for material evaluation but also for designing tire structures to resist premature failure. These conclusions disagree with those from an earlier investigation, so the causes for the discrepancies are examined and discussed.

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Dynamics of the parachute sling - Testing procedures and evaluations

10.2514/6.1991-846 ◽

1991 ◽

Author(s):

D. PREVORSEK ◽

H. CHIN ◽

Y. KWON

Keyword(s):

Testing Procedures

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Hubungan Tingkat Pengetahuan dengan Presisi dan Akurasi Pemipetan Menggunakan Mikropipet Metode Forward

Jurnal Laboratorium Khatulistiwa ◽

10.30602/jlk.v2i1.318 ◽

2019 ◽

Vol 2 (1) ◽

pp. 5

Author(s):

Hendra Budi Sungkawa ◽

Inten Ayu Kusuma

Keyword(s):

Random Sampling ◽

Simple Random Sampling ◽

Support Service ◽

Control Area ◽

Test Results ◽

Medical Support ◽

Testing Procedures ◽

Service Unit ◽

Level Of Knowledge ◽

Precision And Accuracy

Abstract : Pipetting is one of the most important activities in health laboratory analysis. Knowledge of pipetting must be owned by every health laboratory. Students of health analyst as a candidate of health laboratory who will become medical support service unit is expected to be able to do the pipetting precision and accurately. To get a thorough and accurate results then the results of the analysis must be located within a specifc control area and both in precision and accuracy. Precision and accuracy are responsible for analytical interpretation of test results and testing procedures. The method use in this research is the forward method by 61 people research samples which determined by simple random sampling. While the research design used was observational analytics. Based on the results of the research obtained the level of knowledge of respondents research is 71,38; precision pipetting 99,69% and accuracy pipetting 99,58%. From the data that has been obtained then analyzed statistically using tau kendau test. The result indicates that the level of knowledge and precision has a p=0,640 and correlation coeffcient of -0,044 so that the Ho is accepted, meaning there is no relationship between the level of knowledge and precision meaningful. Meanwhile, the level of knowledge and accuracy of the p=0,574 and correlation coeffcient value is -0,053 so that Ho is accepted, it means there is no relationship between the level of knowledge with accuracy. Abstrak: Pemipetan merupakan salah satu kegiatan yang sangat penting dalam analisa laboratorium kesehatan. Pengetahuan pemipetan harus dimiliki oleh setiap tenaga laboratorium kesehatan. Mahasiswa analis kesehatan sebagai calon tenaga laboratorium kesehatan yang akan menjadi unit pelayanan penunjang medis diharapkan mampu melakukan pemipetan dengan teliti dan akurat. Untuk mendapatkan hasil yang teliti dan akurat maka hasil analisa harus terletak di dalam daerah kontrol tertentu dan baik dalam presisi maupun akurasi. Presisi dan akurasi bertanggung jawab terhadap interpretasi analitik hasil pengujian serta prosedur pengujian. Metode yang dilakukan dalam penelitian ini adalah metode forward oleh sampel penelitian sebanyak 61 orang yang ditentukan secara simple random sampling. Sedangkan desain penelitian yang digunakan adalah observasional analitik. Berdasarkan dari hasil penelitian diperoleh nilai tingkat pengetahuan responden penelitian adalah 71,38; presisi pemipetan 99,69% dan akurasi pemipetannya 99,58%. Dari data yang telah didapatkan kemudian dianalisis secara statistik menggunakan uji kendal tau. Untuk tingkat pengetahuan dengan presisi didapatkan hasil nilai p=0,640 dan koefsien korelasi -0,044 sehingga Ho diterima, berarti tidak ada hubungan antara tingkat pengetahuan dan presisi. Untuk tingkat pengetahuan dengan akurasi nilai p=0,574 dan koefsien korelasi adalah -0,053 sehingga Ho diterima, berarti tidak ada hubungan antara tingkat pengetahuan dengan akurasi.

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