Endothelial Cell Seeded Synthetic Vascular Grafts

2008 ◽  
pp. 33-33-11 ◽  
Author(s):  
JC Stanley ◽  
LM Graham ◽  
WE Burkel
1996 ◽  
Vol 5 (5) ◽  
pp. 296 ◽  
Author(s):  
Manfred Deutsch ◽  
Johann Meinhart ◽  
Teddy Fischlein ◽  
Peter Zilla

1989 ◽  
Vol 3 (4) ◽  
pp. 351-363 ◽  
Author(s):  
Tiziano Curti ◽  
Gianandrea Pasquinelli ◽  
Paola Preda ◽  
Antonio Freyrie ◽  
Renzo Laschi ◽  
...  

Angiology ◽  
1995 ◽  
Vol 46 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Keith A. Robinson ◽  
Francisco J. Candal ◽  
Neal A. Scott ◽  
Edwin W. Ades

1988 ◽  
Vol 1 (1) ◽  
pp. 35-44 ◽  
Author(s):  
Steven P. Schmidt ◽  
Navid Monajjem ◽  
M. Michelle Evancho ◽  
Todd R. Pippert ◽  
W. V. Sharp

1994 ◽  
Vol 8 ◽  
pp. 139
Author(s):  
J.V. Smyth ◽  
M. Welch ◽  
H.M.H. Carr ◽  
P. Eisenberg ◽  
M.G. Walker

2000 ◽  
Vol 84 (08) ◽  
pp. 325-331 ◽  
Author(s):  
M. J. B. Wissink ◽  
M. J. A. van Luyn ◽  
R. Beernink ◽  
F. Dijk ◽  
A. A. Poot ◽  
...  

SummaryEndothelial cell seeding, a promising method to improve the performance of small-diameter vascular grafts, requires a suitable substrate, such as crosslinked collagen. Commonly used crosslinking agents such as glutaraldehyde and formaldehyde cause, however, cytotoxic reactions and thereby hamper endothelialization of currently available collagen-coated vascular graft materials.The aim of this study was to investigate the effects of an alternative method for crosslinking of collagen, using N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) in combination with N-hydroxysuccinimide (NHS), on various cellular functions of human umbilical vein endothelial cells (HUVECs) in vitro. Compared to non-crosslinked type I collagen, proliferation of seeded endothelial cells was significantly increased on EDC/NHS-crosslinked collagen. Furthermore, higher cell numbers were found with increasing crosslink densities. Neither the morphology of the cells nor the secretion of prostacyclin (PGI2), von Willebrand factor (vWF), tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI-1) was affected by the crosslink density of the collagen substrate. Therefore, EDC/NHScrosslinked collagen is candidate substrate for in vivo application such as endothelial cell seeding of collagen-coated vascular grafts.


2006 ◽  
Vol 7 (3) ◽  
pp. 210
Author(s):  
G.X. Wang ◽  
D.H. Wei ◽  
L.S. Liu ◽  
C.J. Tang ◽  
Y.Z. Yang ◽  
...  

2012 ◽  
Vol 100A (8) ◽  
pp. 2204-2210 ◽  
Author(s):  
Lynn A. Dudash ◽  
Faina Kligman ◽  
Samantha M. Sarett ◽  
Kandice Kottke-Marchant ◽  
Roger E. Marchant

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