Identification of Aneuploidy-Inducing Agents Using the Wheat Seedling Assay

2009 ◽  
pp. 326-326-5
Author(s):  
SS Sandhu ◽  
JS Dhesi ◽  
BS Gill
Keyword(s):  
2020 ◽  
Vol 52 (5) ◽  
Author(s):  
Hongjuan Jiang ◽  
Yi Fu ◽  
Cuixiang Li ◽  
Mengying Chen ◽  
Zewei Gu ◽  
...  

2016 ◽  
Vol 42 (12) ◽  
pp. 1764 ◽  
Author(s):  
Sheng-Hui ZHOU ◽  
Qiu-Hong WU ◽  
Jing-Zhong XIE ◽  
Jiao-Jiao CHEN ◽  
Yong-Xing CHEN ◽  
...  

2000 ◽  
Vol 55 (3-4) ◽  
pp. 175-182 ◽  
Author(s):  
Stewart B Wuest ◽  
Stephan L Albrecht ◽  
Katherine W Skirvin

1997 ◽  
Vol 43 (5) ◽  
pp. 417-424 ◽  
Author(s):  
Toshihide Takasawa ◽  
Keiko Sagisaka ◽  
Koichi Yagi ◽  
Kyoko Uchiyama ◽  
Atsushi Aoki ◽  
...  

A polygalacturonase was isolated from the culture medium of Sclerotinia borealis, a psychrophilic fungus that grows on lawn and wheat seedling under the snow in winter and induces the snow mold disease. Pectic acid was a better substrate of this enzyme than pectin when the activity was determined by measuring the reducing sugar produced. However, when the activity was measured by viscosity change, the viscosity of pectin decreased more rapidly than that of pectic acid. The results of viscosity change apparently indicate that the polygalacturonase catalyzes pectin hydrolysis as an endo-type enzyme. Highly methyl-esterified pectin was a poor substrate, as determined by measurements of reducing sugar production and viscosity change. It is suggested from the results that the methoxy group of pectin affects the polygalacturonase reaction. A reaction mechanism was proposed for the polygalacturonase reaction. Molecular mass of this enzyme was 40 kDa and its isoelectric point was pH 7.5. Optimum pH of the enzyme reaction was 4.5 and its optimum temperature was 40–50 °C. Thirty percent of the maximum activity was observed at 5 °C, but it was only slightly active above 60 °C. The activity was preserved for more than 2 years at 5 °C and pH 4.5, but it was lost when kept at room temperature overnight or heated at 50 °C for 30 min. The amino acid sequence of the N-terminal region of the psychrophilic polygalacturonase of Sclerotinia borealis is compared with those of polygalacturonases of mesophilic fungi. The function of this enzyme against the target plants is discussed with reference to the reaction of polygalacturonases of mesophilic fungi.Key words: polygalacturonase, pectin-hydrolyzing enzyme, psychrophilic fungi, snow mold disease, Sclerotinia borealis.


1973 ◽  
Vol 51 (5) ◽  
pp. 987-988 ◽  
Author(s):  
V. W. Fogle ◽  
D. N. Munns

Biomics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 20-26
Author(s):  
D.R. Maslennikova ◽  
F.M. Shakirova

The study carried out a comparative analysis of the effect of 24 epibrassinolide (EB) and 6-benzylaminopurine (BAP) on the growth and state of the main components of the glutathione system in the roots of wheat seedlings under the action of 2% NaCl, which for the first time revealed the ability of these phytohormones to similarly stabilize stress-induced decrease GSH / GSSG ratio, positively regulate the activity of glutathione reductase and glutathione-S-transferase. A comparable level of protective effect of BAP and EB on root length was revealed. The data obtained indicate that endogenous cytokinins may play the role of hormonal intermediates in the implementation of the protective.


2017 ◽  
Vol 109 (5) ◽  
pp. 2004-2010 ◽  
Author(s):  
William F. Schillinger ◽  
Steven E. Schofstoll ◽  
Timothy A. Smith ◽  
John A. Jacobsen

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