Monitoring Microbial Pathogens and Indicator Microorganisms in Water by Using the Polymerase Chain Reaction and Gene Probes

2009 ◽  
pp. 445-445-10 ◽  
Author(s):  
RM Atlas ◽  
AK Bej ◽  
S McCarty ◽  
J DiCesare ◽  
L Haff
Keyword(s):  
Polymerase Chain Reaction ◽  
Microbial Pathogens ◽  
Chain Reaction ◽  
Indicator Microorganisms ◽  
Gene Probes ◽  
Polymerase Chain

scholarly journals Detection of coliform bacteria in water by polymerase chain reaction and gene probes.

1990 ◽  
Vol 56 (2) ◽  
pp. 307-314 ◽  
Author(s):  
A K Bej ◽  
R J Steffan ◽  
J DiCesare ◽  
L Haff ◽  
R M Atlas
Keyword(s):  
Polymerase Chain Reaction ◽  
Coliform Bacteria ◽  
Chain Reaction ◽  
Gene Probes ◽  
Polymerase Chain

scholarly journals Application of Rapid-Cycle Real-Time Polymerase Chain Reaction for the Detection of Microbial Pathogens: The Mayo-Roche Rapid Anthrax Test

2002 ◽  
Vol 77 (7) ◽  
pp. 673-680 ◽  
Author(s):  
James R. Uhl ◽  
Constance A. Bell ◽  
Lynne M. Sloan ◽  
Mark J. Espy ◽  
Thomas F. Smith ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Microbial Pathogens ◽  
Chain Reaction ◽  
Polymerase Chain

Real-time polymerase chain reaction followed by fast sequencing allows rapid genotyping of microbial pathogens

2010 ◽  
Vol 43 (2) ◽  
pp. 95-99 ◽  
Author(s):  
Tara Wahab ◽  
Johan Ankarklev ◽  
Marianne Lebbad ◽  
Steve Glavas ◽  
Staffan Svärd ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Microbial Pathogens ◽  
Chain Reaction ◽  
Polymerase Chain

Use of polymerase chain reaction for diagnosis of inherited disorders.

1989 ◽  
Vol 35 (9) ◽  
pp. 1843-1848 ◽  
Author(s):  
C D Boehm
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Single Gene ◽  
Prenatal Testing ◽  
Fold Increase ◽  
Microbial Pathogens ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Inherited Disorders ◽  
Polymerase Chain

Abstract The polymerase chain reaction (PCR) is a rapid method for generating a 10(6)- to 10(7)-fold increase in the number of copies of a discrete DNA or RNA sequence. The technique is being used for rapid prenatal diagnosis and carrier testing of several inherited disorders. After PCR, mutations producing single-gene disorders can be detected by several different methods, including endonuclease digestion and gel electrophoresis (applicable when a mutation affects an endonuclease recognition site), gel electrophoresis (used for detection of deletions), and hybridization to an oligonucleotide probe specific for a mutation. Less often, gene sequencing of a PCR product is used to rapidly identify a mutation. In addition, the PCR technique can be applied to polymorphism analysis to provide diagnosis by linkage analysis. In other areas, PCR is being used to detect and characterize microbial pathogens and to characterize mutations associated with carcinogenesis. The PCR method is useful in situations in which the amount of DNA sample is limited, such as in forensics and prenatal testing, or in which the quality of the DNA sample is poor.

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