Chlorophyll Fluorescence: Its Status and Future as a Rapid Assay of Plant Stress

2009 ◽  
pp. 123-123-11
Author(s):  
LA Kapustka
Keyword(s):  
Chlorophyll Fluorescence ◽  
Plant Stress ◽  
Rapid Assay

scholarly journals MEASUREMENT OF SUN INDUCED CHLOROPHYLL FLUORESCENCE USING HYPERSPECTRAL SATELLITE IMAGERY

2016 ◽  
Vol XLI-B8 ◽  
pp. 911-913
Author(s):  
S. M. Irteza ◽  
J. E. Nichol
Keyword(s):  
Chlorophyll Fluorescence ◽  
Steady State ◽  
Satellite Images ◽  
Plant Stress ◽  
Fluorescence Signal ◽  
Absorption Bands ◽  
Considerable Evidence ◽  
Different Seasons ◽  
Insight Into ◽  
Accurate Indicator

Solar Induced Chlorophyll Fluorescence (SIF), can be used as an indicator of stress in vegetation. Several scientific approaches have been made and there is considerable evidence that steady state Chlorophyll fluorescence is an accurate indicator of plant stress hence a reliable tool to monitor vegetation health status. Retrieval of Chlorophyll fluorescence provides an insight into photochemical and carbon sequestration processes within vegetation. Detection of Chlorophyll fluorescence has been well understood in the laboratory and field measurement. Fluorescence retrieval methods were applied in and around the atmospheric absorption bands 02B (Red wavelength) approximately 690 nm and 02A (Far red wavelengths) 740 nm. Hyperion satellite images were acquired for the years 2012 to 2015 in different seasons. Atmospheric corrections were applied using the 6S Model. The Fraunhofer Line Discrimanator (FLD) method was applied for retrieval of SIF from the Hyperion images by measuring the signal around the absorption bands in both vegetated and non vegetated land cover types. Absorption values were extracted in all the selected bands and the fluorescence signal was detected. The relationships between NDVI and Fluorescence derived from the satellite images are investigated to understand vegetation response within the absorption bands.

Photoprotection in water-stressed plants of durum wheat (Triticum turgidum var. durum): changes in chlorophyll fluorescence, spectral signature and photosynthetic pigments

2002 ◽  
Vol 29 (1) ◽  
pp. 35 ◽  
Author(s):  
Eduardo A. Tambussi ◽  
Jaume Casadesus ◽  
Sergi Munné-Bosch ◽  
José Luis Araus
Keyword(s):  
Chlorophyll Fluorescence ◽  
Water Stress ◽  
Durum Wheat ◽  
Triticum Turgidum ◽  
Plant Stress ◽  
Co2 Assimilation ◽  
Photochemical Quenching ◽  
Spectral Signature ◽  
Antioxidant Defences ◽  
Carotene Content

We analysed the photoprotective response in water-stressed plants of durum wheat (Triticum turgidum L. var. durum cv. Mexa). The plants were grown in a greenhouse for 4 weeks and then exposed to water stress by withholding water for 8 d. Development of water stress was monitored as the decrease in relative water content (RWC) and net CO2 assimilation of the last fully developed leaf. The photoprotective response was evaluated in the same leaves by analysing modulated chlorophyll fluorescence, leaf spectroradiometrical changes, and pigment content. Measurements were performed 3, 6 (moderate stress) and 8 (severe stress) d after water-stress treatment began. The non-photochemical quenching of chlorophyll fluorescence (qN), as well as the contents of zeaxanthin and antheraxanthin increased significantly after 6 d of treatment. However, a further rise in these xanthophylls on day 8 was not associated with any increase in qN. In addition, the β-carotene content rose significantly on day 8, suggesting an increase in antioxidant defences. The photochemical index (PI), derived from spectroradiometrical measurements, showed a strong progressive drop on days 6 and 8, which was paralleled by an increase in the de-epoxidation state of the xanthophyll cycle (DPS), in particular by the zeaxanthin content. At midday, PI was strongly (negatively) correlated with DPS and qN. These results suggest that the PI may be a reliable indicator of photoprotection in the study of plant stress, and in breeding programs.

Plant stress analysis: Application of prompt, delayed chlorophyll fluorescence and 820 nm modulated reflectance. Insights from independent experiments

2014 ◽  
Vol 85 ◽  
pp. 105-113 ◽  
Author(s):  
Elisabetta Salvatori ◽  
Lina Fusaro ◽  
Elena Gottardini ◽  
Martina Pollastrini ◽  
Vasilij Goltsev ◽  
...  
Keyword(s):  
Chlorophyll Fluorescence ◽  
Stress Analysis ◽  
Plant Stress ◽  
Analysis Application

scholarly journals MEASUREMENT OF SUN INDUCED CHLOROPHYLL FLUORESCENCE USING HYPERSPECTRAL SATELLITE IMAGERY

2016 ◽  
Vol XLI-B8 ◽  
pp. 911-913
Author(s):  
S. M. Irteza ◽  
J. E. Nichol
Keyword(s):  
Chlorophyll Fluorescence ◽  
Steady State ◽  
Satellite Images ◽  
Plant Stress ◽  
Fluorescence Signal ◽  
Absorption Bands ◽  
Considerable Evidence ◽  
Different Seasons ◽  
Insight Into ◽  
Accurate Indicator

Solar Induced Chlorophyll Fluorescence (SIF), can be used as an indicator of stress in vegetation. Several scientific approaches have been made and there is considerable evidence that steady state Chlorophyll fluorescence is an accurate indicator of plant stress hence a reliable tool to monitor vegetation health status. Retrieval of Chlorophyll fluorescence provides an insight into photochemical and carbon sequestration processes within vegetation. Detection of Chlorophyll fluorescence has been well understood in the laboratory and field measurement. Fluorescence retrieval methods were applied in and around the atmospheric absorption bands 02B (Red wavelength) approximately 690 nm and 02A (Far red wavelengths) 740 nm. Hyperion satellite images were acquired for the years 2012 to 2015 in different seasons. Atmospheric corrections were applied using the 6S Model. The Fraunhofer Line Discrimanator (FLD) method was applied for retrieval of SIF from the Hyperion images by measuring the signal around the absorption bands in both vegetated and non vegetated land cover types. Absorption values were extracted in all the selected bands and the fluorescence signal was detected. The relationships between NDVI and Fluorescence derived from the satellite images are investigated to understand vegetation response within the absorption bands.

DEVELOPMENT OF A PORTABLE SPECTROMETER FOR DETECTING PLANT STRESS CONDITIONS

2020 ◽  
Vol 4 (41) ◽  
pp. 104-110
Author(s):  
ALEKSANDR SMIRNOV ◽  
◽  
YURIY PROSHKIN ◽  
IGOR DOVLATOV
Keyword(s):  
Spectral Analysis ◽  
Chlorophyll Fluorescence ◽  
Fluorescence Spectrum ◽  
Plant Stress ◽  
Stress Factor ◽  
Stress Conditions ◽  
Research Purpose ◽  
Portable Devices ◽  
Halogen Lamp ◽  
Stress States

Non-invasive methods for detecting plant stress conditions are a method for registering chlorophyll fluorescence, including measurement of induction and spectral analysis of fluorescence, and a method for spectral analysis of radiation reflected from leaves. Existing portable devices allow you to measure only one of these parameters. (Research purpose) The research purpose is in developing a combined portable device for detecting plant stress conditions by recording the reflective characteristics of leaves, the spectrum, and the induction of chlorophyll fluorescence. (Materials and methods) Authors developed a laboratory sample of the device for determining the stress states of plants and tested it on plants of garden basil (Ocimum basilicum) lemon varieties, while some plants were exposed to a stress factor in the form of ultraviolet C-radiation. Authors analyzed the fluorescence spectrum of chlorophyll when excited by various radiation sources and the back reflection spectrum when irradiated with a halogen lamp. (Results and discussion) The influence of the stress factor is manifested in a change in the shape of the fluorescence spectrum, namely, in a decrease in the intensity of fluorescence in the long-wave part of the spectrum. the reflectivity in the green and far red regions of the spectrum also decreases, and the steepness of the graph decreases when moving from the red to the far red region. (Conclusions) To register the fluorescence spectrum of chlorophyll, the best option for excitation is the use of lasers with wavelengths in the range of 405÷470 nanometers. It is possible to combine the functions of spectral analysis of chlorophyll fluorescence and radiation reflected from leaves in one device, which can improve the accuracy of determining the stress states of plants.

scholarly journals Features and applications of a field imaging chlorophyll fluorometer to measure stress in agricultural plants

2020 ◽  
Author(s):  
Alexander I. Linn ◽  
Alexander K. Zeller ◽  
Erhard E. Pfündel ◽  
Roland Gerhards
Keyword(s):  
Chlorophyll Fluorescence ◽  
Plant Stress ◽  
Pulse Amplitude ◽  
Sensor Data ◽  
Stress Detection ◽  
Herbicide Resistant ◽  
Primary Stress ◽  
Field Estimation ◽  
Agricultural Plants ◽  
Imaging Sensor

Abstract Most non-destructive methods for plant stress detection do not measure the primary stress response but reactions of processes downstream of primary events. For instance, the chlorophyll fluorescence ratio Fv/Fm, which indicates the maximum quantum yield of photosystem II, can be employed to monitor stress originating elsewhere in the plant cell. This article describes the properties of a sensor to quantify herbicide and pathogen stress in agricultural plants for field applications by the Fv/Fm parameter. This dedicated sensor is highly mobile and measures images of pulse amplitude modulated (PAM) chlorophyll fluorescence. Special physical properties of the sensor are reported, and the range of its field applications is defined. In addition, detection of herbicide resistant weeds by employing an Fv/Fm-based classifier is described. The PAM-imaging sensor introduced here can provide in-field estimation of herbicide sensitivity in crops and weeds after herbicide treatment before any damage becomes visible. Limitations of the system and the use of a classifier to differentiate between stressed and non-stressed plants based on sensor data are presented. It is concluded that stress detection by the Fv/Fm parameter is suitable as an expert tool for decision making in crop management.

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