Chapter 3—Musculoskeletal Allograft Tissue Banking and Safety

2009 ◽  
pp. 30-30-38
Author(s):  
CT Laurencin ◽  
WW Tomford ◽  
MJ Joyce ◽  
DM Joyce
Keyword(s):  
Tissue Banking ◽  
Allograft Tissue

scholarly journals Initial study on removing cellular residues from hydrostatic high-pressure treated allogeneic tissue using ultrasound

2020 ◽  
Vol 6 (3) ◽  
pp. 176-179
Author(s):  
Christoph Drobek ◽  
Janine Waletzko ◽  
Michael Dau ◽  
Bernhard Frerich ◽  
Volker Weißmann ◽  
...  
Keyword(s):  
High Pressure ◽  
Structural Properties ◽  
Dna Content ◽  
Bone Allograft ◽  
Initial Study ◽  
Microbiological Contamination ◽  
Test Setup ◽  
Allograft Tissue ◽  
Bone Allografts ◽  
Content Determination

AbstractHydrostatic high-pressure technology (HHD) devitalizes tissue quickly and gently, without negatively affecting the structural properties. HHD-treated tissues must be cleaned from devitalized cells. A partially automated, gentle, reproducible and timesaving rinsing test setup utilizing ultrasound is demonstrated in this study. The test setup is used to clean HHD-treated bone allografts of tissue residues and prevent microbiological contamination. A rinsing procedure is investigated. Residual DNA content determination is utilized to analyze cleaned bone allograft tissue for rinsing procedure evaluation.

scholarly journals Current standards in tissue banking

2010 ◽  
Vol 5 (n1) ◽  
pp. 148-154 ◽  
Author(s):  
A. Pruss ◽  
H.-J. Mönig
Keyword(s):  
Tissue Banking ◽  
Current Standards

A Novel Process for Optimizing Musculoskeletal Allograft Tissue to Improve Safety, Ultrastructural Properties, and Cell Infiltration

2012 ◽  
Vol 94 (16) ◽  
pp. 1458-1467 ◽  
Author(s):  
Patrick W. Whitlock ◽  
Thorsten M. Seyler ◽  
Griffith D. Parks ◽  
David A. Ornelles ◽  
Thomas L. Smith ◽  
...  
Keyword(s):  
Cell Infiltration ◽  
Allograft Tissue

The tissue banking in cancer and stem cell research

2007 ◽  
Vol 212 (2) ◽  
pp. 345-347 ◽  
Author(s):  
Antonio Graziano ◽  
Ida Biunno ◽  
Pasquale De Blasio ◽  
Antonio Giordano
Keyword(s):  
Stem Cell ◽  
Stem Cell Research ◽  
Tissue Banking

scholarly journals What Factors Influence the Biomechanical Properties of Allograft Tissue for ACL Reconstruction? A Systematic Review

2017 ◽  
Vol 475 (10) ◽  
pp. 2412-2426 ◽  
Author(s):  
Drew A. Lansdown ◽  
Andrew J. Riff ◽  
Molly Meadows ◽  
Adam B. Yanke ◽  
Bernard R. Bach
Keyword(s):  
Systematic Review ◽  
Acl Reconstruction ◽  
Biomechanical Properties ◽  
Allograft Tissue

scholarly journals Adipose Derived-Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Ilaria Roato ◽  
Daniela Alotto ◽  
Dimas Carolina Belisario ◽  
Stefania Casarin ◽  
Mara Fumagalli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Articular Cartilage ◽  
Tissue Banking ◽  
Stromal Vascular Fraction ◽  
Single Infusion ◽  
Knee Arthritis ◽  
Symptomatic Knee

Osteoarthritis is characterized by loss of articular cartilage also due to reduced chondrogenic activity of mesenchymal stem cells (MSCs) from patients. Adipose tissue is an attractive source of MSCs (ATD-MSCs), representing an effective tool for reparative medicine, particularly for treatment of osteoarthritis, due to their chondrogenic and osteogenic differentiation capability. The treatment of symptomatic knee arthritis with ATD-MSCs proved effective with a single infusion, but multiple infusions could be also more efficacious. Here we studied some crucial aspects of adipose tissue banking procedures, evaluating ATD-MSCs viability, and differentiation capability after cryopreservation, to guarantee the quality of the tissue for multiple infusions. We reported that the presence of local anesthetic during lipoaspiration negatively affects cell viability of cryopreserved adipose tissue and cell growth of ATD-MSCs in culture. We observed that DMSO guarantees a faster growth of ATD-MSCs in culture than trehalose. At last, ATD-MSCs derived from fresh and cryopreserved samples at −80°C and −196°C showed viability and differentiation ability comparable to fresh samples. These data indicate that cryopreservation of adipose tissue at −80°C and −196°C is equivalent and preserves the content of ATD-MSCs in Stromal Vascular Fraction (SVF), guaranteeing the differentiation ability of ATD-MSCs.

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