Eliminating Wasteful “Cut and Try” Procedures in Gas Chromatography Method Development Using Computer Modeling

1996 ◽  
Vol 24 (3) ◽  
pp. 143
Author(s):  
DR Petersen ◽  
RE Link ◽  
R Annino ◽  
R Villalobos
Keyword(s):  
Gas Chromatography ◽  
Computer Modeling ◽  
Method Development ◽  
Chromatography Method

Brazilian southeast brown propolis: gas chromatography method development for its volatile oil analysis, its antimicrobial and leishmanicidal activities evaluation

2020 ◽  
Vol 32 (3) ◽  
pp. 404-411
Author(s):  
Victor Pena Ribeiro ◽  
Caroline Arruda ◽  
Jennyfer Andrea Aldana Mejía ◽  
Ana Carolina Bolela Bovo Candido ◽  
Raquel Alves Santos ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Method Development ◽  
Volatile Oil ◽  
Oil Analysis ◽  
Chromatography Method

scholarly journals Method development and validation for the determination of residual solvents in quinabut API by using gas chromatography. Message 2

Pharmacia ◽  
2021 ◽  
Vol 68 (1) ◽  
pp. 53-59
Author(s):  
Оlena Golembiovska ◽  
Oleksii Voskoboinik ◽  
Galina Berest ◽  
Sergiy Kovalenko ◽  
Liliya Logoyda
Keyword(s):  
Gas Chromatography ◽  
Method Development ◽  
Limit Of Detection ◽  
Accurate Method ◽  
Chromatography Method ◽  
Synthetic Process ◽  
Residual Solvent ◽  
Residual Solvents ◽  
All Solutions ◽  
Limit Of Quantitation

Aim. The aim of study was to develop and validate a simple, precise and accurate method using gas chromatography for analysis of residual solvents – acetone and 2-propanol – in quinabut API. Materials and methods. All experiments were performed on a gas chromatographic system equipped with FID detector (Shimadzu GC System) using the DB-624 (30 m × 0.32 mm ID, 3.0 μm film sickness) column as stationary phase. Nitrogen was used as carrier gas with flow rate 7.5 mL/ min. Split ratio was 1:5, injector temperature was 140 °C, detector temperature was 250 °C, oven temperature was programmed from 40 °C (2 min) to 50 °C at 1 °C/min and then increased at a rate of 15 °C/min up to 215 °C; and maintained for 2 min. All solutions were prepared using water as diluent. Results. This proposed method is assessed for separation of residual solvent from quinabut with quantification. The obtained results are compared with the corresponding specified limits of ICH standard guidelines. The method validation was done by evaluating specificity, limit of detection (LOD) and limit of quantitation (LOQ), linearity, accuracy, repeatability, ruggedness, system suitability and method precision of residual solvents as indicated in the ICH harmonized tripartite guideline. The separation between acetone and 2-propanol peaks is 2.07. Hence method was found to be specific. The linear relationship evaluated across range of 15 to 180% for acetone and 2-propanol of ICH specified limit of residual solvents. The graphs of theoretical concentration versus obtained concentration are linear and the regression coefficients ‘R’ for residual solvents were more than 0.9968. The values of LOD and LOQ were much less than the lower limit of the concentration range and cannot affect the accuracy of the test. The technique was characterized by high intra-laboratory accuracy at concentrations close to the nominal acetone and 2-propanol concentration. All solutions were stable in water for at least 1 hour when stored at room temperature. Conclusion. A simple, specific, accurate, precise and rugged gas chromatography method was developed and validated for the quantification of residual solvents present in quinabut API through an understanding of the synthetic process, nature of solvents and nature of stationary phases of columns. The residual solvents acetone and 2-propanol were determined.

scholarly journals ANALYSIS OF DETERMINING CONCENTRATION OF 3QUINUCLIDINOL IN DRUG SUBSTANCES

2020 ◽  
Vol 2 (2) ◽  
pp. 60-65
Author(s):  
Abdul Qadeer ◽  
Yaseen Jan
Keyword(s):  
Gas Chromatography ◽  
Method Development ◽  
Limit Of Detection ◽  
Sodium Hydroxide Solution ◽  
Drug Substance ◽  
Limit Of Quantification ◽  
Chromatography Method ◽  
Drug Substances ◽  
Solifenacin Succinate ◽  
Validation Criteria

The goal of the study was to determine low level concentrations of 3-quinuclidinol in solifenacin succinate drug substance by using gas chromatography system. 3-quinuclidinol was used as an intermediate in the process of synthesis of solifenacin succinate. The method development was initiated with solifenacin succinate, solubility of 3-quinuclidinol, extraction and miscibility studies, chosen with 6 N sodium hydroxide solution and chloroform solvents. The method of the study was validated based on the guidelines provided by ICH. The criteria were method precision, robustness, accuracy, linearity, limit of quantification, limit of detection, and individuality in terms of specificity. In conclusion, in the present study, we developed a reliable gas chromatography method which was validated based on 3quinuclidinol in solifenacin succinate drug substance. Findings of different validation criteria used shows that the proposed method in this study is accurate, robust, precise, linear, sensitive, and specific.

Sign in / Sign up

Export Citation Format

Share Document