Stable Isotope Ratios as a Tool in Microbial Forensics—Part 1. Microbial Isotopic Composition as a Function of Growth Medium

2004 ◽  
Vol 49 (5) ◽  
pp. 1-7 ◽  
Author(s):  
Helen W. Kreuzer-Martin ◽  
Lesley A. Chesson ◽  
Michael J. Lott ◽  
Janet V. Dorigan ◽  
James R. Ehleringer
Keyword(s):  
Stable Isotope ◽  
Isotopic Composition ◽  
Growth Medium ◽  
Isotope Ratios ◽  
Stable Isotope Ratios ◽  
Microbial Forensics

scholarly journals Stable Isotope Ratios and Forensic Analysis of Microorganisms

2007 ◽  
Vol 73 (12) ◽  
pp. 3896-3908 ◽  
Author(s):  
Helen W. Kreuzer-Martin ◽  
Kristin H. Jarman
Keyword(s):  
Bacillus Subtilis ◽  
Stable Isotope ◽  
Growth Medium ◽  
Water Samples ◽  
Culture Media ◽  
Isotope Ratios ◽  
Forensic Analysis ◽  
Stable Isotope Ratios ◽  
Production Environment ◽  
Microbial Forensics

ABSTRACT In the aftermath of the anthrax letters of 2001, researchers have been exploring various analytical signatures for the purpose of characterizing the production environment of microorganisms. One such signature is stable isotope ratios, which in heterotrophs, are a function of nutrient and water sources. Here we discuss the use of stable isotope ratios in microbial forensics, using as a database the carbon, nitrogen, oxygen, and hydrogen stable isotope ratios of 247 separate cultures of Bacillus subtilis 6051 spores produced on a total of 32 different culture media. In the context of using stable isotope ratios as a signature for sample matching, we present an analysis of variations between individual samples, between cultures produced in tandem, and between cultures produced in the same medium but at different times. Additionally, we correlate the stable isotope ratios of carbon, nitrogen, oxygen, and hydrogen for growth medium nutrients or water with those of spores and show examples of how these relationships can be used to exclude nutrient or water samples as possible growth substrates for specific cultures.

Stable Isotope Ratios as a Tool in Microbial Forensics—Part 3. Effect of Culturing on Agar-containing Growth Media

2005 ◽  
Vol 50 (6) ◽  
pp. 1-8 ◽  
Author(s):  
Helen W. Kreuzer-Martin ◽  
Lesley A. Chesson ◽  
Michael J. Lott ◽  
James R. Ehleringer
Keyword(s):  
Stable Isotope ◽  
Isotope Ratios ◽  
Growth Media ◽  
Stable Isotope Ratios ◽  
Microbial Forensics

TRANSFER EFFICIENCY FROM PRIMARY PRODUCERS TO RUDITAPES PHILIPPINARUM ON AN INTERTIDAL FLAT IN HIROSHIMA BAY, JAPAN

2017 ◽  
Author(s):  
Sosuke Otani ◽  
Sosuke Otani ◽  
Akira Umehara ◽  
Akira Umehara ◽  
Haruka Miyagawa ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Secondary Production ◽  
Isotope Ratios ◽  
Ruditapes Philippinarum ◽  
Transfer Efficiency ◽  
Food Sources ◽  
Stable Isotope Ratios ◽  
Primary Producers ◽  
Nitrogen Stable Isotope ◽  
Carbon And Nitrogen

Fish yields of Ruditapes philippinarum have been decreased and the resources have not yet recovered. It needs to clarify food sources of R. philippinarum, and relationship between primary and secondary production of it. The purpose on this study is to reveal transfer efficiency from primary producers to R. philippinarum and food sources of R. philippinarum. The field investigation was carried out to quantify biomass of R. philippinarum and primary producers on intertidal sand flat at Zigozen beach in Hiroshima Bay, Japan. In particular, photosynthetic rates of primary producers such as Zostera marina, Ulva sp. and microphytobenthos were determined in laboratory experiments. The carbon and nitrogen stable isotope ratios for R. philippinarum and 8 potential food sources (microphytobenthos, MPOM etc) growing in the tidal flat were also measured. In summer 2015, the primary productions of Z. marina, Ulva sp. and microphytobenthos were estimated to be 70.4 kgC/day, 43.4 kgC/day and 2.2 kgC/day, respectively. Secondary production of R. philippinarum was 0.4 kgC/day. Contribution of microphytobenthos to R. philippinarum as food source was 56-76% on the basis of those carbon and nitrogen stable isotope ratios. Transfer efficiency from microphytobenthos to R. philippinarum was estimated to be 10-14%. It was suggested that microphytobenthos might sustain the high secondary production of R. philippinarum, though the primary production of microphytobenthos was about 1/10 compared to other algae.

scholarly journals Stable Isotope Ratios of Herbs and Spices Commonly Used as Herbal Infusions in the Italian Market

ACS Omega ◽  
2021 ◽  
Author(s):  
Purna K. Khatri ◽  
Roberto Larcher ◽  
Federica Camin ◽  
Luca Ziller ◽  
Agostino Tonon ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Ratios ◽  
Stable Isotope Ratios ◽  
Italian Market ◽  
Herbal Infusions ◽  
Herbs And Spices

scholarly journals Elemental and Isotopic Characterization of Tobacco from Umbria

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 186
Author(s):  
Luana Bontempo ◽  
Daniela Bertoldi ◽  
Pietro Franceschi ◽  
Fabio Rossi ◽  
Roberto Larcher
Keyword(s):  
Stable Isotope ◽  
Economic Value ◽  
Isotope Ratios ◽  
Stable Isotope Ratios ◽  
Umbria Region ◽  
Geographical Regions ◽  
Isotopic Characterization ◽  
First Time ◽  
Micro Elements

Umbrian tobacco of the Virginia Bright variety is one of the most appreciated tobaccos in Europe, and one characterized by an excellent yield. In recent years, the Umbria region and local producers have invested in introducing novel practices (for production and processing) focused on environmental, social, and economic sustainability. Due to this, tobacco from Umbria is a leading commodity in the global tobacco industry, and it claims a high economic value. The aim of this study is then to assess if elemental and isotopic compositions can be used to protect the quality and geographical traceability of this particular tobacco. For the first time the characteristic value ranges of the stable isotope ratios of the bio-elements as a whole (δ2H, δ13C, δ15N, δ18O, and δ34S) and of the concentration of 56 macro- and micro-elements are now available, determined in Virginia Bright tobacco produced in two different areas of Italy (Umbria and Veneto), and from other worldwide geographical regions. The ranges of variability of elements and stable isotope ratios had slightly different results, according to the three geographical origins considered. In particular, Umbria samples presented significantly lower content of metals potentially dangerous for human health. The results of this first exploratory work highlight the possibility of characterizing tobacco from Umbria, and suggest widening the scope of the survey throughout Italy and foreign regions, in order to be used to describe the geographical origin of tobacco in general and verify the origin of the products on the market.

Variations in stable isotope ratios in lamb blood fractions following dietary changes: a preliminary study

2015 ◽  
Vol 30 (1) ◽  
pp. 170-174 ◽  
Author(s):  
L. Bontempo ◽  
F. Camin ◽  
L. Ziller ◽  
L. Biondi ◽  
M. G. D'Urso ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Isotope Ratios ◽  
Stable Isotope Ratios ◽  
Dietary Changes ◽  
Preliminary Study
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