Variation of the Hypervariable Region-1 of Mitochondrial DNA in Central-Eastern Italy

2003 ◽  
Vol 48 (2) ◽  
pp. 2002350 ◽  
Author(s):  
F. Verginelli ◽  
F. Donati ◽  
V. Coia ◽  
I. Boschi ◽  
R. Palmirotta ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Hypervariable Region ◽  
Hypervariable Region 1 ◽  
Central Eastern

scholarly journals Mitochondrial DNA hypervariable region 1 diversity in Nigerian goats – CORRIGENDUM

2017 ◽  
pp. 1
Author(s):  
Moses Okpeku ◽  
Sunday O. Peters ◽  
Ikhide G. Imumorin ◽  
Kyle C. Caires ◽  
Varun K. Sharma ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Hypervariable Region ◽  
Hypervariable Region 1

Mitochondrial DNA hypervariable region-1 sequence variation and phylogeny of the concolor gibbons,Nomascus

2007 ◽  
Vol 69 (11) ◽  
pp. 1285-1306 ◽  
Author(s):  
Keri Monda ◽  
Rachel E. Simmons ◽  
Philipp Kressirer ◽  
Bing Su ◽  
David S. Woodruff
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Variation ◽  
Hypervariable Region ◽  
Hypervariable Region 1

Mitochondrial DNA hypervariable region 1 polymorphism in Singapore Chinese population

2005 ◽  
Vol 7 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Cheng-Yap Shee ◽  
Michelle S.M. Chong ◽  
Irene Ng ◽  
Tet-Fatt Chia
Keyword(s):  
Mitochondrial Dna ◽  
Chinese Population ◽  
Hypervariable Region ◽  
Hypervariable Region 1 ◽  
Singapore Chinese

scholarly journals Haplogroup Determination Using Hypervariable Region 1 and 2 of Human Mitochondrial DNA

2014 ◽  
Vol 14 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Sahidan Senafi ◽  
Shahrul Hisham Zainal Ari ◽  
Rus Dina Rus Din ◽  
Rohaya Megat Abdul Waha ◽  
Intan Zarina Zainol Abi ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Hypervariable Region ◽  
Human Mitochondrial Dna ◽  
Hypervariable Region 1

scholarly journals Effects of Hair dyes on Sequence Analysis of Hair Mitochondrial DNA Hypervariable Region 1

2017 ◽  
Vol 11 (1) ◽  
pp. 61-66
Author(s):  
Halah Al-summarraie ◽  
Mohammed Al-Zubaidi ◽  
Dhuha Salim نعمة ◽  
Sura Nabeel Hameed ◽  
Thooalnoon Younes Saleh ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Sequence Analysis ◽  
Dna Extraction ◽  
Hypervariable Region ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Hair Dyes ◽  
Hypervariable Region 1 ◽  
Hair Samples ◽  
The Impact

Hair can be a valuable source of DNA especially in forensic casework and for noninvasive studies of human, when blood samples not available. This study emphasizes the impact of hair dyes on DNA sequence analysis. Samples collected from forty Iraqi families; each sample was divided in to two parts hair follicles and hair shaft. DNA extracted by using two different techniques, Phenol-chloroform (organic) method and prepFiler forensic DNA extraction kit. After quantification of DNA by real time PCR to confirm the exact DNA yield, Mitochondrial DNA (MtDNA) hypervariable region 1 successfully amplified from (50% of samples include hair follicle and 20% samples include shaft only), which all extracted  by organic method. Whereas by using prepFiler kit the ratio of amplification success reach to 95% of samples included hair follicles, but there wais no DNA outcome from hair shaft by using this kit. Our results demonstrate that treated hair by dyed or henna had a significant influence on the sequence analysis results. Organic method was an appropriate method for extraction DNA from hair shaft, since this method used for extracting the old and degraded samples. While prepFiler DNA extraction kit was more convenient for isolation DNA from, hair samples included follicles only with excellent result.

scholarly journals Mitochondrial DNA variation and phylogeography of native Mongolian goats

2020 ◽  
Vol 33 (6) ◽  
pp. 902-912 ◽  
Author(s):  
Onolragchaa Ganbold ◽  
Seung-Hwan Lee ◽  
Woon Kee Paek ◽  
Munkhbaatar Munkhbayar ◽  
Dongwon Seo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Population Structure ◽  
Genetic Differentiation ◽  
Nucleotide Diversity ◽  
Demographic History ◽  
Hypervariable Region ◽  
Dna Variation ◽  
Hypervariable Region 1 ◽  
Phylogenetic Status

Objective: Mongolia is one of a few countries that supports over 25 million goats, but genetic diversity, demographic history, and the origin of goat populations in Mongolia have not been well studied. This study was conducted to assess the genetic diversity, phylogenetic status and population structure of Mongolian native goats, as well as to discuss their origin together with other foreign breeds from different countries using hypervariable region 1 (HV1) in mtDNA.Methods: In this study, we examined the genetic diversity and phylogenetic status of Mongolian native goat populations using a 452 base-pair long fragment of HVI of mitochondrial DNA from 174 individuals representing 12 populations. In addition, 329 previously published reference sequences from different regions were included in our phylogenetic analyses.Results: Investigated native Mongolian goats displayed relatively high genetic diversities. After sequencing, we found a total of 109 polymorphic sites that defined 137 haplotypes among investigated populations. Of these, haplotype and nucleotide diversities of Mongolian goats were calculated as 0.997±0.001 and 0.0283±0.002, respectively. These haplotypes clearly clustered into four haplogroups (A, B, C, and D), with the predominance of haplogroup A (90.8%). Estimates of pairwise differences (Fst) and the analysis of molecular variance values among goat populations in Mongolia showed low genetic differentiation and weak geographical structure. In addition, Kazakh, Chinese (from Huanghuai and Leizhou), and Arabian (Turkish and Baladi breeds) goats had smaller genetic differentiation compared to Mongolian goats.Conclusion: In summary, we report novel information regarding genetic diversity, population structure, and origin of Mongolian goats. The findings obtained from this study reveal that abundant haplogroups (A to D) occur in goat populations in Mongolia, with high levels of haplotype and nucleotide diversity.

Mitochondrial DNA hypervariable region 1 diversity in Nigerian goats

2016 ◽  
Vol 59 ◽  
pp. 47-54 ◽  
Author(s):  
Moses Okpeku ◽  
Sunday O. Peters ◽  
Ikhide G. Imumorin ◽  
Kyle C. Caires ◽  
Varun K. Sharma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Demographic History ◽  
Hypervariable Region ◽  
West African ◽  
High Genetic Diversity ◽  
Hypervariable Region 1 ◽  
Selection For ◽  
Protein Supply ◽  
Appreciable Variation

SummaryGoats make up the largest group of ruminant livestock in Nigeria and are strategic in bridging animal protein supply gap and improving the economy of rural households. The hypervariable region 1 (HVR1) of the caprine mitochondrial genome was investigated to better understand genetic diversity important for improving selection for animal breeding and conservation programs. We sequenced and analysed the mitochondrial DNA (mtDNA) HVR1 in 291 unrelated indigenous Nigerian goats (West African Dwarf (WAD), Red Sokoto (RSO) and Sahel (SAH)), randomly sampled from around the country, and compared them with the HVR1 sequences of 336 Indian goats and 12 other sequences in five different species in the genusCapra(C. falconeri, C. ibex nubiana, C. aegagrus, C. cylindricornisandC. sibirica). A total of 139 polymorphic sites from 291 individuals were captured in 204 haplotypes. Within and among population variations were 77.25 and 22.74 percent, respectively. Nigerian goats showed high genetic diversity (0.87) and high FST values, and separate from Indian goats and other wild species. Haplogroups in WAD separates it from RSO and SAH concomitant with a different demographic history. Clear genetic structure was found among Nigerian goat breeds with appreciable variation in mtDNA HVR1 region. This study grouped Nigerian goat breeds into two major groups suggesting two different demographic origins for Northern and Southern breeds. High genetic admixing denotes different maternal origins and in contrast to evidence from goats from Levant and Central Asia, where goats were originally domesticated.

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