Performance Evaluation of Two Multiplexes Used in Fluorescent Short Tandem Repeat DNA Analysis

2003 ◽  
Vol 48 (2) ◽  
pp. 2001316 ◽  
Author(s):  
Edward L. Buse ◽  
Jeanne C. Putinier ◽  
Mary M. Hong ◽  
Aimee E. Yap ◽  
John M. Hartmann
Keyword(s):  
Performance Evaluation ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Analysis ◽  
Repeat Dna ◽  
Short Tandem

DNA analysis in disputed parentage: The occurrence of two apparently false exclusions of paternity, both at short tandem repeat (STR) loci, in the one child

1997 ◽  
Vol 18 (9) ◽  
pp. 1650-1652 ◽  
Author(s):  
Peter R. Gunn ◽  
Katherine Trueman ◽  
Patricia Stapleton ◽  
Derryck B. Klarkowski
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Analysis ◽  
Str Loci ◽  
The One ◽  
Short Tandem

The HumD21S11 system of short tandem repeat DNA polymorphisms in Japanese and Chinese

1997 ◽  
Vol 86 (1-2) ◽  
pp. 109-118 ◽  
Author(s):  
H-G Zhou ◽  
K Sato ◽  
Y Nishimaki ◽  
L Fang ◽  
H Hasekura
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Polymorphisms ◽  
Repeat Dna ◽  
Short Tandem

Fetus-in-fetu in the cranium of a 4-month-old boy: histopathology and short tandem repeat polymorphism–based genotyping

2008 ◽  
Vol 1 (5) ◽  
pp. 410-414 ◽  
Author(s):  
Jin Wook Kim ◽  
Sung-Hye Park ◽  
Sung-Sup Park ◽  
Kyu-Chang Wang ◽  
Byung-Kyu Cho ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Analysis ◽  
Ganglion Cells ◽  
Rare Condition ◽  
Histopathological Analysis ◽  
Fetus In Fetu ◽  
Well Differentiated ◽  
Short Tandem ◽  
Tandem Repeat Polymorphism

✓Fetus-in-fetu is a very rare condition in which one fetus is contained within another. About 100 cases have been reported, and in most of these the fetus was located in the retroperitoneum. The authors describe an extremely rare case of an intracranial fetus-in-fetu in an extraaxial location. This is the eighth intracranial fetus-in-fetu to be reported, the first intracranial extraaxial case, and involves the oldest documented patient with this condition. Histopathological analysis of the mass revealed a degenerated amnionic membranelike tissue, well-differentiated extremities (including fingerlike structures), skin, matured lungs, well-formed intestines, cerebellar and cerebral tissue, and a notochord with ganglion cells. DNA analysis using short tandem repeat polymorphisms confirmed that the fetus-in-fetu mass and the host infant had heterozygous alleles and were of identical sex and genotype.

scholarly journals The use of touch DNA analysis in forensic identification focusing on Short Tandem Repeat-Combined DNA Index System loci THO1, CSF1PO and TPOX

2020 ◽  
Author(s):  
Ahmad Yudianto ◽  
Indah Nuraini M. ◽  
Abdul Hadi Furqoni ◽  
Simon Martin Manyanza Nzilibili ◽  
Pudji Harjanto
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Index System ◽  
Dna Analysis ◽  
Forensic Identification ◽  
Buccal Swab ◽  
Dna Index ◽  
Touch Dna ◽  
Composite Gel ◽  
Short Tandem

Forensic identification through DNA analysis is an accurate diagnostic tool. Deoxyribonucleic Acid (DNA) analysis is via DNA repetitive regions with less than 1 kb base size is called ‘microsatellite’ or Short Tandem Repeat (STR). At the crime scene, the perpetrator’s skin may accidentally be in contact with surrounding objects, thereby transferring trace evidence to the objects. In this study DNA was obtained using “touch DNA” from two buccal smears and two smear from watches and cellphones from volunteers who had signed the consent form. Samples were isolated using DNAzol. The quantity of DNA obtained will be measured using a UV spectrophotometer. For DNA amplification using 3 STR CODIS loci namely TH01, CSF1PO, and TPOX. The last step is visualization using acrylamide gel and silver staining. Mean levels of DNA (UVVisible Spectrophotometer) were 167.89±85.71 μg/mL for the buccal swab, 59.19±5.58 μg/mL for the watch swab, and 38.09±2.12 μg/mL for the mobile swab; the purity of the buccal swab DNA was 1.79±0.71, of the watch swab 1.69±0.76, and of the mobile swab 1.53±0.56. Visualization of PCR products on Polyacrylamide Agarose Composite Gel Electrophoresis stained with Silver and amplified using the standard primers THOI, TPOX and CSF1PO for STR Combined DNA Index System (CODIS) showed a 100% detection of amplicons. Both the buccal swab, watch swab and handphone swabs had trace amount of DNA that was sufficient to be isolated and amplified by using Polymerase Chain Reaction on the STR CODIS loci THO1, CSF1PO and TPOX.

Identification of Exhumed Remains of Fire Tragedy Victims Using Conventional Methods and Autosomal/Y-Chromosomal Short Tandem Repeat DNA Profiling

2005 ◽  
Vol 26 (3) ◽  
pp. 285-291 ◽  
Author(s):  
Gayvelline C. Calacal ◽  
Frederick C. Delfin ◽  
Michelle Music M. Tan ◽  
Lutz Roewer ◽  
Danilo L. Magtanong ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Profiling ◽  
Repeat Dna ◽  
Conventional Methods ◽  
Short Tandem
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