Sex Determination of Forensic Samples by Simultaneous PCR Amplification of α-Satellite DNA from Both the X and Y Chromosomes

1995 ◽  
Vol 40 (2) ◽  
pp. 15349J ◽  
Author(s):  
Dieter Neeser ◽  
Sabina Liechti-Gallati
Keyword(s):  
Sex Determination ◽  
Satellite Dna ◽  
Pcr Amplification ◽  
Y Chromosomes ◽  
Forensic Samples

Sex determination of forensic samples by dual PCR amplification of an X-Y homologous gene

1992 ◽  
Vol 52 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Atsushi Akane ◽  
Satoko Seki ◽  
Hiroshi Shiono ◽  
Hiroaki Nakamura ◽  
Masanori Hasegawa ◽  
...  
Keyword(s):  
Sex Determination ◽  
Pcr Amplification ◽  
Homologous Gene ◽  
Forensic Samples

scholarly journals Sex Determination of Equine Somatic and Germ Cells by PCR Amplification Based on the Sequence Polymorphism of X- and Y-Chromosomal Amelogenin Genes

1999 ◽  
Vol 70 (7) ◽  
pp. 6-10 ◽  
Author(s):  
Yasutoshi FUKUSHIMA ◽  
Harutaka MUKOYAMA ◽  
Fumio SATO ◽  
Telhisa HASEGAWA ◽  
Nobushige ISHIDA ◽  
...  
Keyword(s):  
Sex Determination ◽  
Germ Cells ◽  
Pcr Amplification ◽  
Sequence Polymorphism

Monotreme sex chromosomes - implications for the evolution of amniote sex chromosomes

2009 ◽  
Vol 21 (8) ◽  
pp. 943 ◽  
Author(s):  
Paul D. Waters ◽  
Jennifer A. Marshall Graves
Keyword(s):  
Sex Determination ◽  
Sex Chromosomes ◽  
Sex Chromosome ◽  
Female Development ◽  
Comparative Gene Mapping ◽  
Y Chromosomes ◽  
Daunting Task ◽  
Platypus Genome ◽  
Genetic Events

In vertebrates, a highly conserved pathway of genetic events controls male and female development, to the extent that many genes involved in human sex determination are also involved in fish sex determination. Surprisingly, the master switch to this pathway, which intuitively could be considered the most critical step, is inconsistent between vertebrate taxa. Interspersed in the vertebrate tree there are species that determine sex by environmental cues such as the temperature at which eggs are incubated, and then there are genetic sex-determination systems, with male heterogametic species (XY systems) and female heterogametic species (ZW systems), some of which have heteromorphic, and others homomorphic, sex chromosomes. This plasticity of sex-determining switches in vertebrates has made tracking the events of sex chromosome evolution in amniotes a daunting task, but comparative gene mapping is beginning to reveal some striking similarities across even distant taxa. In particular, the recent completion of the platypus genome sequence has completely changed our understanding of when the therian mammal X and Y chromosomes first arose (they are up to 150 million years younger than previously thought) and has also revealed the unexpected insight that sex determination of the amniote ancestor might have been controlled by a bird-like ZW system.

264 SUITABILITY OF BOVINE SEXING PRIMERS FOR SEX DETERMINATION OF CAPRINE CELLS

2008 ◽  
Vol 20 (1) ◽  
pp. 212
Author(s):  
C. Herr ◽  
C. Valentin ◽  
K. Greeson
Keyword(s):  
Sex Determination ◽  
Dna Sequences ◽  
Restriction Site ◽  
Pcr Amplification ◽  
Single Copy ◽  
Repeat Sequence ◽  
Agarose Gel ◽  
Specific Sequence ◽  
Taq Polymerase

Recent studies on PCR-based sex determination of caprine cells use amplification of a single-copy Y-chromosome-specific sequence. Our study investigated the suitability of widely used bovine sexing primers for their usefulness in sexing caprine cells. The common use of these sexing primers is to amplify BRY.4, a Y-specific sequence found in bovine cells (Reed et al. 1995 U.S. Pat# 5459038). The major advantage in amplifying these sequences is that they are multiple repeated DNA sequences. This enhances the sensitivity for use on as few as one cell. Early in their use, a problem was discovered amplifying BRY.4 for bovine sexing; one or more copies of a homologous repeat sequence were present in female cells. Copies in female cells differed by the presence of a Hinf1 restriction site. By digesting the DNA with Hinf1 either prior or subsequent to PCR amplification, it was possible to eliminate the troublesome sequence. Our study investigated whether a site homologous to theY-specific sequence was also present in female caprine cells and, if so, it too contained the Hinf1 restriction site, making possible the elimination of the troublesome sequence. The components of the PCR reaction mix were: buffer (50 mm Tris, 1% dextran T-500, 50 mm KCl, 2.5 mm MgCl2, and 0.035% 2- mercaptoethanol), deoxyribonucleotides (5 µm) (Boehringer Mannheim, Basel, Switzerland), primers (5´-GAACTTTCAAGCAGCTGAGGC-3´ and 5´-GATTGTTGATCCCACAGAAGG-3´) (2.5 µm) (custom synthesis, IDT Inc, Coralville, IA, USA), and Taq polymerase (2.7 U) (AmpliTaq DNA Polymerase, Stoeffel Fragment, Perkin Elmer, Branchburg, NJ, USA). Lymphocytes from one male and three female goat species (Saanen, Nubian/Alpine mix, and Albino) were used as the source of DNA. Approximately 100 cells/2 µL were used to ensure the amplification of the sequence homologous to theY-repeat. The DNA replication was carried out using a Corbett Rapid Thermocycler (Model FTS-IS, Corbett Research, Montlake, Australia) in 20-µL volumes, and all assays were run with positive and negative DNA controls. After replication, some samples were digested with Hinf1 (20 U) (New England Biolabs, Ipswich, MA, USA) for 2 h at 38�C. A 3% agarose gel was run in an agarose gel electrophoresis apparatus (Model 52000, IBI-Shelton Scientific, Peosta, IA, USA) for 30 min at 180 volts. All caprine species samples were run in duplicate. The DNA from three female undigested samples banded at the same position as the male samples (130 bp). The DNA from all three female digested samples banded at two positions (50 bp and 80 bp), both lower than the male samples. The male DNA remained unaltered after the Hinf1 digest, which suggests that the Hinf1 site is not present in the amplified DNA from male cells. The assay generated strong sexing bands with as few as 6 male lymphocytes, the lowest number of cells tested. These primers seem suitable if used in conjunction with Hinf1 digestion.

scholarly journals Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

2019 ◽  
Vol 3 (1) ◽  
pp. 17-22
Author(s):  
Ismail A. Mohammed ◽  
Israa A. Mohamed
Keyword(s):  
Sex Determination ◽  
Date Palm ◽  
Early Stage ◽  
Pcr Amplification ◽  
Issr Markers ◽  
Ctab Method ◽  
Male Specific ◽  
Rapd And Issr ◽  
Issr Primers

Abstract Date palm is one of the most important economical crops in the world. Sex determination of date palm in early stage is a prerequisite for breeding and cultivation. The aim of this study is to validate RAPD and ISSR markers for sex identification of date palm genotypes grown under Sudan conditions. DNA was extracted from ten seedlings and five male and female plants using CTAB method. Eight primers, six RAPD and two ISSR primers were examined for their validation in sex determination of date palm genotypes. PCR amplification was performed using these primers. Four RAPD primers OPA02, OPJ-09, RD A02 and RD A21 were amplified male specific band with size of 1000, 1100, 1000 and 1400 pb respectively, while ISSR markers could not. The specific bands were observed clearly among all male genotypes and absent in female samples unknown samples irrespective of genotypes. Our results could be useful for sex determination of date palm sex in seedling stage and would promote date palm cultivation and production.

SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT

2014 ◽  
Vol 13 (1) ◽  
pp. 50-59
Author(s):  
A NisreenYasirJasim ◽  
Tahir A. Fahid ◽  
Talib Ahmed Jaayid
Keyword(s):  
Sex Determination ◽  
Maternal Plasma ◽  
Duplex Pcr
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