Determination of ABO Blood Grouping from Human Oral Squamous Epithelium by the Highly Sensitive Immunohistochemical Staining Method EnVision+

2002 ◽  
Vol 47 (2) ◽  
pp. 15254J ◽  
Author(s):  
Hiroshi Noda ◽  
Makoto Yokota ◽  
Shinji Tatsumi ◽  
Shizuyuki Sugiyama
Keyword(s):  
Immunohistochemical Staining ◽  
Squamous Epithelium ◽  
Staining Method ◽  
Highly Sensitive ◽  
Abo Blood Grouping ◽  
Blood Grouping

scholarly journals Determination of ABO blood grouping from dentine and pulp by absorption-elution technique

2018 ◽  
Vol 8 (2) ◽  
pp. 102
Author(s):  
AbhishekSingh Nayyar ◽  
KV Chalapathi ◽  
G Kartheek ◽  
K Pramod ◽  
AbhishekRanjan Pati ◽  
...  
Keyword(s):  
Abo Blood Grouping ◽  
Blood Grouping

scholarly journals Determination of ABO blood grouping and Rhesus factor from tooth material

2016 ◽  
Vol 20 (3) ◽  
pp. 540
Author(s):  
PoojaVijay Kumar ◽  
M Vanishree ◽  
K Anila ◽  
Santosh Hunasgi ◽  
SriSujan Suryadevra ◽  
...  
Keyword(s):  
Rhesus Factor ◽  
Abo Blood Grouping ◽  
Blood Grouping

scholarly journals Expression of EpCam and Villin in Barrett’s Esophagus and in Gastric Cardia

2008 ◽  
Vol 24 (6) ◽  
pp. 287-292 ◽  
Author(s):  
Mario Anders ◽  
Mario Sarbia ◽  
Carsten Grotzinger ◽  
Alexander Meining ◽  
Heinz Hofler ◽  
...  
Keyword(s):  
Intestinal Metaplasia ◽  
Immunohistochemical Staining ◽  
Squamous Epithelium ◽  
Intraepithelial Neoplasia ◽  
Weak Signal ◽  
Esophageal Epithelium ◽  
Cardiac Mucosa ◽  
Barrett Carcinoma

In the current study we aimed to clarify the potential of EpCAM and villin asin vivobiomarkers for both Barrett esophagus (BE)-associated neoplasia and BE versus cardiac mucosa. Immunohistochemical staining in BE with various degrees of intraepithelial neoplasia (IN), Barrett carcinoma (BC) and in normal cardiac mucosa (CM) revealed a lack of EpCam and villin in squamous esophageal epithelium. All specimens of IN and BC showed EpCam with varying staining intensities. In 57% of CM samples a weak signal was detected; the remainder displayed strong EpCam expression. Villin was found in 97% of BE specimens and in all those with IN; 37% of BC and 75% of CM specimens were also positive. We conclude that expression of EpCam and villin differs only between squamous epithelium and BE. Determination of these proteins does not allow discrimination between different degrees of neoplasia or between esophageal intestinal metaplasia and cardiac mucosa.

scholarly journals ABO Blood Grouping of Non-medullated Scalp Hair Using Immunohistochemical Staining

2010 ◽  
Vol 15 (1) ◽  
pp. 39-48
Author(s):  
Tomoko Akutsu ◽  
Hiroshi Ikegaya ◽  
Ken Watanabe ◽  
Koichi Sakurada
Keyword(s):  
Immunohistochemical Staining ◽  
Scalp Hair ◽  
Abo Blood Grouping ◽  
Blood Grouping

Comparison of localization of metallothionein in tissues of metallothionein-deficient mice

1995 ◽  
Vol 53 ◽  
pp. 1042-1043
Author(s):  
H. Nishimura ◽  
R Nishimura ◽  
D.L. Adelson ◽  
A.E. Michaelska ◽  
K.H.A. Choo ◽  
...  
Keyword(s):  
Metal Binding ◽  
Immunohistochemical Staining ◽  
Squamous Epithelium ◽  
Rabbit Antiserum ◽  
Slight Modification ◽  
Positive Control ◽  
Heavy Metal Binding ◽  
Critical Organ ◽  
Metal Binding Protein ◽  
Deficient Mice

Metallothionein (MT), a cysteine-rich heavy metal binding protein, has several isoforms designated from I to IV. Its major isoforms, I and II, can be induced by heavy metals like cadmium (Cd) and, are present in various organs of man and animals. Rodent testes are a critical organ to Cd and it is still a controversial matter whether MT exists in the testis although it is clear that MT is not induced by Cd in this tissue. MT-IV mRNA was found to localize within tongue squamous epithelium. Whether MT-III is present mainly glial cells or neurons has become a debatable topic. In the present study, we have utilized MT-I and II gene targeted mice and compared MT localization in various tissues from both MT-deficient mice and C57Black/6J mice (C57BL) which were used as an MT-positive control. For MT immunostaining, we have used rabbit antiserum against rat MT-I known to cross-react with mammalian MT-I and II and human MT-III. Immunohistochemical staining was conducted by the method described in the previous paper with a slight modification after the tissues were fixed in HistoChoice and embedded in paraffin.

ABO Blood Grouping of Human Hair by Universal Immuno-Peroxidase Polymer (UIP) Technique

2003 ◽  
Vol 7 (2) ◽  
pp. 145-153
Author(s):  
Yasunari Hisashi ◽  
Tatsuo Shinozuka ◽  
Osamu Ohue ◽  
Setsuko Takei ◽  
Ayako Ro ◽  
...  
Keyword(s):  
Human Hair ◽  
Abo Blood Grouping ◽  
Blood Grouping

Controlled Growth and Characterization Ag/ZnO Nanotetrapods for Humidity Sensing

2018 ◽  
Vol 21 (7) ◽  
pp. 462-467
Author(s):  
Babak Sadeghi
Keyword(s):  
Room Temperature ◽  
Zinc Complex ◽  
Water Solution ◽  
Particle Morphology ◽  
Quick Response ◽  
Separation System ◽  
Humidity Sensing ◽  
Sensor Material ◽  
Highly Sensitive

Aim and Objective: Ultrafine Ag/ZnO nanotetrapods (AZNTP) have been prepared successfully using silver (I)–bis (oxalato) zinc complex and 1, 3-diaminopropane (DAP) with a phase separation system, and have been injected into a diethyl/water solution. Materials and Methods: This crystal structure and lattice constant of the AZNTP obtained were investigated by means of a SEM, XRD, TEM and UV-vis spectrum. Results: The results of the present study demonstrated the growth and characterization AZNTP for humidity sensing and DAP plays a key role in the determination of particle morphology. AZNTP films with 23 nm in arm diameter have shown highly sensitive, quick response sensor material that works at room temperature.

Simultaneous Determination of Methocarbamol and Paracetamol in the Presence of Three Related Substances by Ultra Performance Liquid Chromatography

2019 ◽  
Vol 15 (5) ◽  
pp. 505-510
Author(s):  
Yanjuan Zheng ◽  
Qiushi Peng ◽  
Rui Dong ◽  
Tingyu Chen ◽  
Yi Bao ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pharmaceutical Preparations ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Related Substances ◽  
Bulk Powder ◽  
Highly Sensitive ◽  
Optimal Protocol ◽  
Acquity Uplc

Introduction: A rapid, and accurate Ultra Performance Liquid Chromatography (UPLC) method was developed to simultaneously analyze Methocarbamol, Paracetamol and the related substances Materials and Methods: Waters ACQUITY UPLC® BEH Phenyl C18 column was used in conjunction with UV detection at 225nm. Gradient elution with 0.05M, pH 6 phosphate buffer and acetonitrile flow at 0.3mL /min rate were used to separate the substances. The retention times for 4-Aminopheno, Paracetamol, Guaifenesin, Methocarbamol, and 4-Chloroacetanilide were 1.319 minute, 2.224 minute, 4.467 minute, 4.769 minute and 5.433 minute respectively. The concentration was linear in the range of 2-100 µg/ml for Methocarbamol, and 1-100 µg/mL for Paracetamol. The percentage recoveries were between 99.28±1.23% to 100.57±0.99% for Methocarbamol, and between 99.08±1.23% to 101.23±1.39% for Paracetamol. Results and Discussion: The validated optimal protocol is robust and accurate for simultaneous analysis of Methocarbamol, Paracetamol and the related substances, applicable for bulk powder as well as pharmaceutical formulation. Conclusion: In this paper, a highly sensitive, accurate, and precise UPLC method with UV-Vis detection was developed and validated for quality control of MET and PAR in bulk as well as in pharmaceutical preparations.

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