Identification of Ammunitions Used in a Lethal Robbery. Comparison Between Scanning Electron Microscopy/Energy Dispersive X-Ray Analysis (SEM-EDX) and Instrumental Neutron Activation Analysis (INAA) Measurements

1993 ◽  
Vol 38 (5) ◽  
pp. 13530J ◽  
Author(s):  
Antonio Moauro ◽  
Giacomo Falso
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Neutron Activation Analysis ◽  
Instrumental Neutron Activation Analysis ◽  
Activation Analysis ◽  
Neutron Activation ◽  
Energy Dispersive ◽  
X Ray ◽  
Scanning Electron ◽  
Instrumental Neutron Activation

Correlative Instrumental Neutron Activation Analysis, Light Microscopy, Transmission Electron Microscopy, and X-ray Microanalysis for Qualitative and Quantitative Detection of Colloidal Gold Spheres in Biological Specimens

1998 ◽  
Vol 4 (5) ◽  
pp. 481-490 ◽  
Author(s):  
Julián F. Hillyer ◽  
Ralph M. Albrecht
Keyword(s):  
Electron Microscopy ◽  
Neutron Activation Analysis ◽  
Instrumental Neutron Activation Analysis ◽  
Activation Analysis ◽  
Neutron Activation ◽  
Colloidal Gold ◽  
Quantitative Detection ◽  
X Ray ◽  
Instrumental Neutron Activation

Colloidal gold, conjugated to ligands or antibodies, is routinely used as a label for the detection of cell structures by light (LM) and electron microscopy (EM). To date, several methods to count the number of colloidal gold labels have been employed with limited success. Instrumental neutron activation analysis (INAA), a physical method for the analysis of the elemental composition of materials, can be used to provide a quantitative index of gold accumulation in bulk specimens. Given that gold is not naturally found in biological specimens in any substantial amount and that colloidal gold and ligand conjugates can be prepared to yield uniform bead sizes, the amount of label can be calculated in bulk biological samples by INAA. Here we describe the use of INAA, LM, transmission EM, and X-ray microanalysis (EDX) in a model to determine both distribution (localization) and amount of colloidal gold at the organ, tissue, cellular, and ultrastructural levels in whole animal systems following administration. In addition, the sensitivity for gold in biological specimens by INAA is compared with that of inductively coupled plasma-mass spectrometry (ICP-MS). The correlative use of INAA, LM, TEM, and EDX can be useful, for example, in the quantitative and qualitative tracking of various labeled molecular species following administration in vivo.

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