A Polymerase Chain Reaction (PCR) Method for Sex and Species Determination with Novel Controls for Deoxyribonucleic Acid (DNA) Template Length

1992 ◽  
Vol 37 (1) ◽  
pp. 13207J ◽  
Author(s):  
R. E. Gaensslen ◽  
Karen M. Berka ◽  
Dina A. Grosso ◽  
Gualberto Ruano ◽  
Elaine M. Pagliaro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Deoxyribonucleic Acid ◽  
Chain Reaction ◽  
Species Determination ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Dna Template

scholarly journals Potensi Formulasi Sediaan Sabun Padat Minyak Kelapa dengan Pengisi Bentonit sebagai Media Pembersih Najis Mughallazah

2021 ◽  
Vol 10 (1) ◽  
pp. 31-37
Author(s):  
Maria Grace Tobing ◽  
Lilis Sukeksi ◽  
Iriany ◽  
Siswarni
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Temperature ◽  
Deoxyribonucleic Acid ◽  
Fatty Acid Content ◽  
Chain Reaction ◽  
Quality Requirements ◽  
Operation Conditions ◽  
Pcr Method ◽  
Polymerase Chain

Najis mughallazah is excrement which comes from pigs which earthen soap can use to purify it.  Method to purify unclean that is necessary to use water seven times and the addition of bentonite to soap is expected to be able to remove unclean Deoxyribonucleic Acid (DNA) that is located on the surface of human skin. The purpose of this study was to determine the effect of the amount of bentonite filler and the reaction temperature on the quality of soap, knowing whether the soap formula meets the SNI quality requirements and knowing whether the soap formula can remove Pig DNA residues using the Polymerase Chain Reaction (PCR) analytical method. In this study, the operation conditions were designed at the reaction temperature (50 oC, 60 oC, 70 oC and 80 oC), bentonite consistency (10%, 12.5%, 15%, 17.5% and 20%), 35% NaOH concentration, reaction time 10 minutes and stirring speed 250 rpm. The analyzes carried out in this study include analysis of water content, free alkaline content, free fatty acid content and PCR method. The best results were obtained for 15% (70 °C) soap that had soap hardness characteristics close to conventional soap and 17.5% (50 °C) soap with the characteristics of soap that could remove najis mughallazah. The resulting solid bentonite soap formula meets the SNI 3523: 2016 standard and can eliminate mughallazah unclean.

scholarly journals Human papillomavirus deoxyribonucleic acid may not be detected in non-genital benign papillomatous skin lesions by polymerase chain reaction

2014 ◽  
Vol 59 (4) ◽  
pp. 334 ◽  
Author(s):  
Davoodi Kaveh ◽  
Ayatollahi Hossein ◽  
Ghanadan Alireza ◽  
Damavandi Maede ◽  
Aghazadeh Nessa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Deoxyribonucleic Acid ◽  
Skin Lesions ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Diversity ofLeptospiraspp. in Rats and Environment from Urban Areas of Sarawak, Malaysia

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Chai Fung Pui ◽  
Lesley Maurice Bilung ◽  
Kasing Apun ◽  
Lela Su’ut
Keyword(s):  
Polymerase Chain Reaction ◽  
Water Samples ◽  
Urban Areas ◽  
Environmental Samples ◽  
Soil Samples ◽  
Chain Reaction ◽  
Prevalence Studies ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Soil And Water

Various prevalence studies onLeptospirain animals and humans, as well as environmental samples, had been conducted worldwide, including Malaysia. However, limited studies have been documented on the presence of pathogenic, intermediate, and saprophyticLeptospirain selected animals and environments. This study was therefore conducted to detectLeptospiraspp. in rats, soil, and water from urban areas of Sarawak using the polymerase chain reaction (PCR) method. A total of 107 rats, 292 soil samples, and 324 water samples were collected from April 2014 to February 2015. PathogenicLeptospirawas present in 5.6% (6/107) of rats, 11.6% (34/292) of soil samples, and 1.9% (6/324) of water samples. IntermediateLeptospirawas present in 2.7% (8/292) of soil samples and 1.9% (6/324) of water samples. SaprophyticLeptospirawas present in 10.3% (11/107) of rats, 1.4% (4/292) of soil samples, and 0.3% (1/324) of water samples. From this study, 76Leptospiraspp. were isolated. Based on DNA sequencing, the dominantLeptospiraspp. circulating in urban areas of Sarawak are pathogenicLeptospira noguchii, intermediateLeptospira wolffiiserovar Khorat, and saprophyticLeptospira meyeri, respectively. Overall, this study provided important surveillance data on the prevalence ofLeptospiraspp. from rats and the environment, with dominant local serovars in urban areas of Sarawak.

Quantitative Real-Time Polymerase Chain Reaction Detection of BK Virus Using Labeled Primers

2010 ◽  
Vol 134 (3) ◽  
pp. 444-448 ◽  
Author(s):  
Zhengming Gu ◽  
Jianmin Pan ◽  
Matthew J. Bankowski ◽  
Randall T. Hayden
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Polymerase Chain Reaction ◽  
Bk Virus ◽  
Clinical Samples ◽  
Quantitative Detection ◽  
Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain

Abstract Context.—BK virus infections among immunocompromised patients are associated with disease of the kidney or urinary bladder. High viral loads, determined by quantitative polymerase chain reaction (PCR), have been correlated with clinical disease. Objective.—To develop and evaluate a novel method for real-time PCR detection and quantification of BK virus using labeled primers. Design.—Patient specimens (n = 54) included 17 plasma, 12 whole blood, and 25 urine samples. DNA was extracted using the MagNA Pure LC Total Nucleic Acid Isolation Kit (Roche Applied Science, Indianapolis, Indiana); sample eluate was PCR-amplified using the labeled primer PCR method. Results were compared with those of a user-developed quantitative real-time PCR method (fluorescence resonance energy transfer probe hybridization). Results.—Labeled primer PCR detected less than 10 copies per reaction and showed quantitative linearity from 101 to 107 copies per reaction. Analytical specificity of labeled primer PCR was 100%. With clinical samples, labeled primer PCR demonstrated a trend toward improved sensitivity compared with the reference method. Quantitative assay comparison showed an R2 value of 0.96 between the 2 assays. Conclusions.—Real-time PCR using labeled primers is highly sensitive and specific for the quantitative detection of BK virus from a variety of clinical specimens. These data demonstrate the applicability of labeled primer PCR for quantitative viral detection and offer a simplified method that removes the need for separate oligonucleotide probes.

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