Hae III—A Suitable Restriction Endonuclease for Restriction Fragment Length Polymorphism Analysis of Biological Evidence Samples

1990 ◽  
Vol 35 (3) ◽  
pp. 12858J ◽  
Author(s):  
Bruce Budowle ◽  
John S. Waye ◽  
Gary G. Shutler ◽  
F. Samuel Baechtel
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Endonuclease ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Biological Evidence ◽  
Suitable Restriction ◽  
Restriction Fragment Length

Astrovirus detection in wastewater samples

2001 ◽  
Vol 43 (12) ◽  
pp. 73-76 ◽  
Author(s):  
R. M. Pintó ◽  
C. Villena ◽  
F. Le Guyader ◽  
S. Guix ◽  
S. Calallero ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Endonuclease ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Southern France ◽  
Wastewater Samples ◽  
Restriction Fragment Length

Procedures for the detection of astroviruses in wastewater samples have been developed and evaluated. Following these methodologies, we investigated the occurrence of astroviruses in wastewater samples from three different sewage treatments plants located in Southern France and two in the Barcelona area. Some positive samples were genotyped by analysis of a fragment of the ORF1a by restriction fragment length polymorphism (RFLP) with endonuclease Dde I. The amplimers generated contain several sites for the DdeI restriction endonuclease, being the number and location of sites different between strains.

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