A Study of Sex Identification of Trace, Dried Bloodstains Using a Y-Chromosome-Specific Deoxyribonucleic Acid (DNA) Probe

1989 ◽  
Vol 34 (2) ◽  
pp. 12643J ◽  
Author(s):  
Zhong-Nian He ◽  
Xean-Hua Jiang ◽  
Shi-Hui Lu ◽  
Guo-Lin Wang ◽  
Yu-Wen Zhu ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Deoxyribonucleic Acid ◽  
Sex Identification ◽  
Dna Probe

scholarly journals Rapid sexing of native amniotic cells by hybridization to a cloned Y chromosome DNA probe

1986 ◽  
Vol 14 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Peter Wieacker ◽  
Clemens R. Müller ◽  
Jan W. Siebers
Keyword(s):  
Y Chromosome ◽  
Dna Probe ◽  
Amniotic Cells

Organization and heterogeneity of sequences within a repeating unit of human Y chromosome deoxyribonucleic acid

Biochemistry ◽  
1979 ◽  
Vol 18 (15) ◽  
pp. 3343-3353 ◽  
Author(s):  
Louis M. Kunkel ◽  
Kirby D. Smith ◽  
Samuel H. Boyer
Keyword(s):  
Y Chromosome ◽  
Deoxyribonucleic Acid ◽  
Human Y Chromosome

scholarly journals Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens

2015 ◽  
Vol 16 (9) ◽  
pp. 727-732
Author(s):  
Zoubeida AL Yahfoufi ◽  
Wahib Hadchiti ◽  
Antoine Berberi
Keyword(s):  
Deoxyribonucleic Acid ◽  
Periodontal Diseases ◽  
Detection Threshold ◽  
High Sensitivity ◽  
Cost Effective ◽  
Dna Probe ◽  
Microbial Pathogens ◽  
Periodontal Pathogens ◽  
Culture Methods ◽  
Low Level

ABSTRACT Background In clinical microbiology several techniques have been used to identify bacteria. Recently, Deoxyribonucleic acid (DNA)-based techniques have been introduced to detect human microbial pathogens in periodontal diseases. Deoxyribonucleic acid probes can detect bacteria at a very low level if we compared with the culture methods. These probes have shown rapid and cost-effective microbial diagnosis, good sensitivity and specificity for some periodontal pathogens in cases of severe periodontitis. Materials and methods Eighty-five patients were recruited for the study. Twenty-one subjects ranging between 22 and 48 years of age fulfilled the inclusion and exclusion criteria. Seventy-eight samples became available for DNA probe analysis from the deepest pockets in each quadrant. Results All 21 patients showed positive results for Prevotella intermedia; also, Prevotella gingivalis was identified in 19 subjects, Aggregatibacter actinomycetemcomitans in 6 subjects. P. intermedia was diagnosed positive in 82% of the subgingival samples taken, 79% for P. gingivalis, and 23% for A. actinomycetemcomitans. Conclusion This study shows a high frequency of putative periodontal pathogens by using DNA probe technology, which is semi-quantitative in this study. Deoxyribonucleic acid probes can detect bacteria at very low level about 103 which is below the detection level of culture methods. The detection threshold of cultural methods. Clinical significance The three types of bacteria can be detected rapidly with high sensitivity by using the DNA probe by general practitioners, and thus can help in the diagnosis process and the treatment. How to cite this article Yahfoufi ZAL, Hadchiti W, Berberi A. Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens. J Contemp Dent Pract 2015;16(9): 727-732.

scholarly journals A sexing protocol for wild ruminants based on PCR amplification of amelogenin genes AMELX and AMELY (short communication)

2007 ◽  
Vol 50 (5) ◽  
pp. 442-446 ◽  
Author(s):  
G. Pajares ◽  
I. Álvarez ◽  
I. Fernández ◽  
L. Pérez-Pardal ◽  
F. Goyache ◽  
...  
Keyword(s):  
Sex Ratio ◽  
Y Chromosome ◽  
X Chromosome ◽  
Short Communication ◽  
Pcr Amplification ◽  
Sex Identification ◽  
Protocol Design ◽  
Wild Ruminant ◽  
Wild Ruminants ◽  
Ruminant Species

Abstract. Based on the sequences of the bovine amelogenin genes, we have designed a protocol for sexing DNA samples of wild ruminants. Basically the protocol consists on the co-amplification of two specific fragments, one from Y-chromosome and one for the X chromosome, making the use of a PCR control unnecessary. It has been demonstrated to be useful for sex identification in a total of 164 samples belonging to six different wild ruminant species. We propose adding to the census procedure commonly based in faecal groups counting, the faecal sampling and application of the protocol design here, to estimate the sex ratio.

366 SEX IDENTIFICATION OF PORCINE EMBRYOS BY PCR BASED ON THE AMELOGENIN GENE

2007 ◽  
Vol 19 (1) ◽  
pp. 298
Author(s):  
S. Senbon ◽  
S.-I. Suzuki ◽  
D.-I. Fuchimoto ◽  
M. Iwamoto ◽  
T. Kawarasaki ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Length Polymorphism ◽  
Dna Sequences ◽  
Genomic Dna ◽  
Mammalian Species ◽  
Sex Identification ◽  
Coding Region ◽  
Salting Out ◽  
Y Chromosomes ◽  
Pcr Products

The amelogenin (AMEL) gene exists on both X and Y chromosomes in various mammalian species. The non-coding region of this gene is different between X and Y chromosomes. The use of this gene has made sex determination much less complicated, since only one pair of primers is required to amplify the different size fragments of the AMEL gene. Therefore, AMEL had been successfully used to determine the sex in cattle, sheep, and humans. The difference of AMEL genomic sequences between X and Y chromosomes has also been found in pig. In this study, we designed primers that identified AMEL of both chromosomes. The amplicons were isolated and sequenced, and showed a length polymorphism characteristic for the X and Y chromosome in pigs. Furthermore we examined whether a single oocyte or embryo could be sexed. Genomic DNA samples were collected from various breeds of pigs (European breeds: Landrace, Large White, Duroc, Berkshire; Chinese breeds: Meishan, Jinhua). DNA was extracted from ears, tails, or leukocytes using the salting-out method and then dissolved in TE buffer. We used one set of primers for amplifying the pig AMEL gene. The polymerase chain reaction (PCR) procedure was performed with initial denaturation at 94�C for 2 min, followed by 40 cycles of one denaturation step at 98�C for 10 s, primer annealing at 60�C for 30 s, and primer extension at 72�C for 30 s in 20 �L of reaction mixture containing 50 ng genomic DNA. The PCR products were electrophoresed and documented. Some amplicons were isolated and sequenced, and showed a length polymorphism characteristic for the X and Y chromosome in every breed. Next, we tried sexing of pig oocytes and embryos. Cumulus–oocyte complexes (COCs) were aspirated from ovaries recovered from prepubertal gilts. COCs were matured in modified NCSU-37 medium for 44 h, fertilized in vitro, and then cultured in PZM5. The pre-implantation embryos were sampled at 1, 2, 3, 4, and 5–6 days after insemination. Day 1–4 embryos were treated in 5 �L of lysis solution; whole solution were used for subsequent PCR. After Day 5–6 of insemination, only blastocysts were treated in 20 �L of lysis solution, and 5 �L were used for PCR. GV oocytes and electro-activated embryos were sampled as controls. PCR amplification yielded the expected 480-bp and 301-bp products. Male pigs in all breeds are expected to show 2 bands (480 bp and 301 bp), whereas all females, one band only (480 bp). The comparison of AMEL gene DNA sequences among pig breeds showed over 99% homology for the PCR products in both the AMEL-X and the AMEL-Y gene, except for several single-base substitutions. Within GV oocytes and electro-activated embryos, 98% and 96–99% of those examined displayed one band of 480 bp. In IVF groups, 49–55% of those embryos had 2 bands, with no difference between the number of embryos displaying one band and two bands. In conclusion, our findings show that the PCR assay based on the AMEL gene is reliable for sex identification in every pig breed. The advantage of this assay is its capability of identifying sex using a genomic DNA sequence as small as that contained within a single cell such as an oocyte.

Use of two different deoxyribonucleic acid probes to detect Y chromosome deoxyribonucleic acid in subjects with normal and altered Y chromosomes

1986 ◽  
Vol 154 (4) ◽  
pp. 737-745 ◽  
Author(s):  
Paul G. McDonough ◽  
Sandra P. Tho ◽  
John J. Trill ◽  
J.Rogers Byrd ◽  
Richard H. Reindollar ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Deoxyribonucleic Acid ◽  
Y Chromosomes

Y Chromosome Specific DNA Probe in the Diagnosis of a Patient with mos 45, X/46, XYnf

1989 ◽  
Vol 31 (1) ◽  
pp. 85-89
Author(s):  
Tohru Yorifuji ◽  
Toru Momoi ◽  
Takako Sonomura ◽  
Chutaro Yamanaka ◽  
Masayuki Kaji ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Dna Probe
Sign in / Sign up

Export Citation Format

Share Document