Practice for Quantification of Calcium Deposits in Osteogenic Culture of Progenitor Cells Using Fluorescent Image Analysis

10.1520/f2997 ◽  
2014 ◽  
Author(s):  
Keyword(s):  
Image Analysis ◽  
Progenitor Cells ◽  
Fluorescent Image ◽  
Calcium Deposits

Multiple Iteractive Labeling by Antibody Neodeposition (MILAN)

2019 ◽  
Author(s):  
Giorgio Cattoretti ◽  
Francesca Maria Bosisio ◽  
Lukas Marcelis ◽  
Maddalena Maria Bolognesi
Keyword(s):  
Image Analysis ◽  
Tissue Section ◽  
Indirect Immunofluorescence ◽  
Analysis Software ◽  
Tissue Sections ◽  
Image Analysis Software ◽  
Single Section ◽  
Fluorescent Image ◽  
Secondary Antibodies

Abstract Multiplexing, labeling for multiple immunostains the very same cell or tissue section in situ, is of considerable interest. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, scarcity of specialized skills or facilities. We have validated and detail here a method based on common primary and secondary antibodies, diffusely available fluorescent image scanners and routinely processed tissue sections \(FFPE). It entails rounds of four-color indirect immunofluorescence, image acquisition and removal \(stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulphide cleavage. In excess of 50 different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software.

scholarly journals Fluorescent image analysis for evaluating the condition of facial sebaceous follicles

2008 ◽  
Vol 14 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Taeyoon Son ◽  
Byungkwan Han ◽  
Byungjo Jung ◽  
J. Stuart Nelson
Keyword(s):  
Image Analysis ◽  
Fluorescent Image

scholarly journals Real-Time Fluorescent Image Analysis of DNA Spot Hybridization Kinetics To Assess Microarray Spot Heterogeneity

2012 ◽  
Vol 84 (21) ◽  
pp. 9379-9387 ◽  
Author(s):  
Archana N. Rao ◽  
Christopher K. Rodesch ◽  
David W. Grainger
Keyword(s):  
Image Analysis ◽  
Real Time ◽  
Fluorescent Image ◽  
Spot Hybridization ◽  
Microarray Spot

5-ALA induced fluorescent image analysis of actinic keratosis

2010 ◽  
Author(s):  
Yong-Jin Cho ◽  
Youngwoo Bae ◽  
Eung-Ho Choi ◽  
Byungjo Jung
Keyword(s):  
Image Analysis ◽  
Actinic Keratosis ◽  
Fluorescent Image

Automated quantitative fluorescent image analysis of cervical cytology

1987 ◽  
Vol 28 (3) ◽  
pp. 241-254 ◽  
Author(s):  
Jeffrey J. Smith ◽  
Linda Cowan ◽  
George Hemstreet ◽  
McClure L. Smith ◽  
Charles H. Pippitt ◽  
...  
Keyword(s):  
Image Analysis ◽  
Cervical Cytology ◽  
Fluorescent Image
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