Practice for Collection of Culturable Airborne Fungi or Bacteria on Agar Plates by Inertial Impaction Systems

2019 ◽  
Author(s):  
Keyword(s):  
Airborne Fungi ◽  
Inertial Impaction

31. Indoor and Outdoor Concentration Levels of Airborne Fungi in Cincinnati Area Homes: Pilot Study

2005 ◽  
Author(s):  
T. Lee ◽  
D. Martuzevicius ◽  
C. Crawford ◽  
A. Adhikari ◽  
T. Reponen ◽  
...  
Keyword(s):  
Pilot Study ◽  
Airborne Fungi ◽  
Outdoor Concentration ◽  
Concentration Levels ◽  
Indoor And Outdoor

scholarly journals Airborne Fungi in Show Caves from Southern Spain

2021 ◽  
Vol 11 (11) ◽  
pp. 5027
Author(s):  
Irene Dominguez-Moñino ◽  
Valme Jurado ◽  
Miguel Angel Rogerio-Candelera ◽  
Bernardo Hermosin ◽  
Cesareo Saiz-Jimenez
Keyword(s):  
Entomopathogenic Fungi ◽  
Airborne Fungi ◽  
Southern Spain ◽  
Ecological Indicator ◽  
Outdoor Air ◽  
Northern Spain ◽  
Pochonia Chlamydosporia ◽  
The North ◽  
Show Caves ◽  
Characteristic Features

The aerobiology of caves in Southern Spain possesses special characteristics, different from caves located in Northern Spain. Previous studies demonstrated the influence of outdoor air on caves in the north and the existence of two different patterns, depending on the season. In summer there is an abundance of Ascomycota, whereas in winter Basidiomycota predominates, which are related to the periods of stagnation and ventilation, respectively. In caves in Southern Spain the presence of airborne Basidiomycota is scarce and Ascomycota represents the main group of fungi widely distributed across the caves in all seasons. The most characteristic features were the abundant presence of entomopathogenic fungi (Beauveria bassiana, Parengyodontium album, Pochonia chlamydosporia, Leptobacillium symbioticum, Leptobacillium leptobactrum) and Cladosporium cladosporioides in Cueva del Tesoro, Cueva de Ardales and Gruta de las Maravillas. However, the presence of yeasts of the genera Cutaneotrichosporon, Trichosporon, Cryptococcus, Naganishia, Cystobasidium, Microstroma and Phragmotaenium was exclusive to Gruta de las Maravillas. Fungal hazard in the three show caves were determined using an ecological indicator based on the concentration of spores in cave air.

scholarly journals First Speleomycological Study on the Occurrence of Psychrophilic and Psychrotolerant Aeromycota in the Brestovská Cave (Western Tatras Mts., Slovakia) and First Reports for Some Species at Underground Sites

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 497
Author(s):  
Rafał Ogórek ◽  
Mateusz Speruda ◽  
Justyna Borzęcka ◽  
Agata Piecuch ◽  
Magdalena Cal
Keyword(s):  
Global Warming ◽  
Immune System ◽  
Biological Material ◽  
Fungal Spores ◽  
Airborne Fungi ◽  
Fungal Species ◽  
Air Samples ◽  
Cold Adapted ◽  
First Report

Most underground ecosystems are heterotrophic, fungi in these objects are dispersed in the air in the form of spores, and they may be potentially hazardous to mammals. Research in underground sites has focused on mesophilic airborne fungi and only a few concerned cold-adapted species. Therefore, the goal of our research was the first report of psychrophilic and psychrotolerant aeromycota in the Brestovská Cave using culture-based techniques with genetic and phenotypic identification. Plates with PDA medium containing sampled biological material were incubated at 8 ± 0.5 °C. The density of mycobiota inside the cave ranged from 37.4 to 71 CFU 1 m−3 of air and 63.3 CFU 1 m−3 of air outside the cave. Thus, the level of fungal spores did not exceed the standards for the mycological quality of the air. A total of 18 species were isolated during the study, and some species may be potentially dangerous to people with weakened immune system. All fungal species were present inside the cave and only seven of them were outside. Cladosporium cladosporioides dominated in the external air samples and Mortierella parvispora was cultured most frequently from internal air samples. To our knowledge, this is the first discovery of the fungal species such as Coniothyrium pyrinum, Cystobasidium laryngis, Filobasidium wieringae, Leucosporidium drummii, M. parvispora, Mrakia blollopis, Nakazawaea holstii, and Vishniacozyma victoriae in the air inside the underground sites. Moreover, C. pyrinum, C. laryngis, L. drummii, M. blollopis, and N. holstii have never been detected in any component of the underground ecosystems. There are possible reasons explaining the detection of those species, but global warming is the most likely.

scholarly journals Diversity, Concentration and Dynamics of Culturable Fungal Bioaerosols at Doha, Qatar

2020 ◽  
Vol 18 (1) ◽  
pp. 182
Author(s):  
Raghdaa K. Fayad ◽  
Roda F. Al-Thani ◽  
Fatima A. Al-Naemi ◽  
Mohammed H. Abu-Dieyeh
Keyword(s):  
Species Richness ◽  
Fungal Spores ◽  
Gravimetric Method ◽  
Fungal Spore ◽  
Industrial Area ◽  
Airborne Fungi ◽  
Culture Collection ◽  
Colony Count ◽  
Study Sites ◽  
Future Work

This research was conducted to investigate the dynamics of airborne fungi using viable culture collection and in respect to different abiotic variables, including seasonal and intra-diurnal variations. A gravimetric method was used to sample airborne fungal deposition on potato dextrose agar plates on alternate days, for a year between April 2015 to March 2016. From 176 settle plate exposures, a total of 1197 mould and 283 yeast colony-forming units (CFU), 21 genera and 62 species were retrieved. The highest fungal spore count was recorded in February 2016, whereas the lowest count occurred in August 2015. The main constituents of the fungal airspora were attributed to Cladosporium (60.2%), Aspergillus (10.4%), Fusarium (9.4%), Alternaria (8.5%), and Ganoderma spp. (2.3%). Temperature was negatively correlated with total colony count (r = −0.231, p ≤ 0.05) or species richness (r = −0.267, p ≤ 0.001), while wind speed was positively correlated with total colony count (r = 0.484, p ≤ 0.001) or species richness (r = 0.257, p ≤ −0.001). The highest dispersal of fungal spores was obtained at 18:00, whereas the lowest fungal spores release was recorded at 00:00 (midnight). There were no significant differences in species composition and richness of the airborne fungal population between two study sites, the Industrial area and Qatar University Campus. The count of Alternaria spp. and Fusarium spp. were significantly higher at the Industrial area site, which corresponds to a higher CO2 level than the Qatar University site. This study lays the foundation for future work to assess the implications of such aeromycological data on public health.

Inertial impaction of fine particles at high temperature and high pressure

1981 ◽  
Vol 12 (4) ◽  
pp. 297-306 ◽  
Author(s):  
Richard Parker ◽  
Seymour Calvert ◽  
Dennis Drehmel ◽  
James Abbott
Keyword(s):  
High Pressure ◽  
High Temperature ◽  
Fine Particles ◽  
Inertial Impaction
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