scholarly journals Effects of Carob Fruit Extract on Spermatogenesis, Antioxidant Status, and Apoptosis in Adult Male Mice

2019 ◽  
Vol 25 (3) ◽  
pp. 184-189
Author(s):  
Seyed Sadegh Sadat ◽  
Shabnam Mohammadi ◽  
Ghasem Sazegar ◽  
Alireza Fazel ◽  
Alireza Ebrahimzadeh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Statistical Analysis ◽  
Adult Male ◽  
Sham Group ◽  
Sperm Parameters ◽  
Male Mice ◽  
Fruit Extract ◽  
Significant Difference ◽  
The Mean ◽  
And Oxidative Stress

Background: Certain plants stimulate spermatogenesis and increase fertility; in contrast, some plants arrest the spermatogenesis cycle. Ceratonia siliqua is an herb plant with a strong antioxidant property. The aim of this study was to evaluate the effects of carob fruit extract on spermatogenesis, testicular apoptosis, and oxidative stress in adult male mice. Methods: Forty adult male mice were randomly divided into five groups: control, sham, and carob 1–3. The sham group was injected with normal saline and the carob 1–3 groups were injected with 200, 400, and 800 mg/kg of the carob fruit extract intraperitoneally for 14 days, respectively. At the end of the injection period, spermatogenesis, testicular apoptosis, and oxidative stress were examined. Results: The sperm parameters increased in the mice that received 200 mg/kg of carob compared to the sham group (p <0.05). There was a significant increase in the weight index of the epididymis in the carob 3 group in comparison to the sham group (p = 0.01). The number of positive tunnel cells was not statistically significant between different groups (p>0.05). The level of malondialdehyde decreased in the carob 1 and carob 3 groups, but this reduction was not statistically significant (p > 0.05). In addition, the statistical analysis showed a significant difference in the mean superoxide dismutase levels in the carob 2 and carob 3 groups in comparison to the sham group (p ≤ 0.001). The statistical analysis showed a significant increase in the mean level of the catalase enzyme in the carob 1 group in comparison to the sham (p = 0.02), and carob 2 groups (p = 0.008). Conclusion: The administration of 200 mg of the carob fruit extract for 14 days increased the testicular index as well as sperm parameters and decreased the level of oxidative stress in the testicular tissue of adult mice.

scholarly journals Effects of Thymoquinone on Sperm Parameters, Apoptosis, Testosterone Level, and Oxidative Stress in a Mouse Model of D-Galactose-Induced Aging

2018 ◽  
Vol 24 (3) ◽  
pp. 180-186 ◽  
Author(s):  
Seyedeh Saleheh Attari ◽  
Shabnam Mohammadi ◽  
Alireza Ebrahimzadeh ◽  
Hossein Hosseinzadeh ◽  
Mohammad Soukhtanloo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mouse Model ◽  
Antioxidant Capacity ◽  
Testosterone Level ◽  
Total Antioxidant Capacity ◽  
Sperm Parameters ◽  
Control Group ◽  
Significant Difference ◽  
Total Antioxidant ◽  
And Oxidative Stress

Background: The aging process is accompanied by low secretion of sex hormones and testicular apoptosis. The antioxidant properties of thymoquinone (TQ) may prevent the effects of aging. Therefore, in the present study, the effects of different doses of TQ were investigated on sperm parameters, testosterone level, apoptosis, and oxidative stress in a mouse model of D-galactose-induced aging. Methods: In this experimental study, 30 adult male mice were randomly divided into 5 groups. The control group did not receive any injections, while the D-galactose group received an intraperitoneal injection of 300 mg/kg of D-galactose for 42 days. The TQ1-TQ3 groups received intraperitoneal injections of 5, 2.5, and 1.25 mg/kg of TQ plus D-galactose, respectively for 14 days (from the 1st to the 14th day of the experiment). The morphometric analysis, testicular apoptosis examination, and sperm analysis were performed, and testosterone level, total antioxidant capacity, and malondialdehyde level were evaluated on day 42 of the experiment. Data were analyzed using SPSS. Results: Administration of TQ in the TQ1 group caused a significant difference in sperm parameters, compared to the D-galactose group (P<0.05). The lowest amount of positive tunnel cells was related to 5 mg/kg of TQ and the highest to 2.5 mg/kg of TQ. There was no significant difference in the parameters of seminal vesicles, epididymis, prostate, and testis between the groups (P>0.05). The malondialdehyde level were decreased in the TQ1-TQ3 groups, compared to the D-galactose group (P<0.001). On the other hand, the total antioxidant capacity was increased significantly in the TQ1 group, compared to the D-galactose group (P<0.001). Conclusion: Administration of 5 mg of TQ for 14 days improved sperm quality and biochemical parameters, while reducing apoptotic cells of the testes in a mouse model of aging.

scholarly journals Comparison of Biochemical and Oxidative Stress Parameters in Hypo and Hyperthyroid Rat Models

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Zohre Najafi ◽  
Asghar Zarban ◽  
Elham Chamani ◽  
Mahdi Honarbakhsh ◽  
Gholamreza Sharifzadeh
Keyword(s):  
Oxidative Stress ◽  
Oxidation State ◽  
Thiol Group ◽  
The Body ◽  
Control Group ◽  
Oxidative Stress Parameters ◽  
Significant Difference ◽  
The Mean ◽  
And Oxidative Stress ◽  
Stress Parameters

Background: Thyroid functional disorders are relatively common in the general population. Different factors play a role in the development of thyroid disorders. These factors can be associated with the deterioration of the oxidation state of the body and the induction of oxidative stress. Thyroid hormones play an important role in the oxidation state. Objectives: Regarding the role of oxidative stress in different pathologies and the development of various complications, including thyroid disorders, we aimed to compare the biochemical and oxidative stress parameters in hypo and hyperthyroid rat models. Methods: Twenty-one male Wistar rats were assigned into three groups of control, hypothyroid, and hyperthyroid. Hypothyroidism and hyperthyroidism were inducted with PTU (propylthiouracil) and LTX (levothyroxine), respectively. After five weeks of induction, serum biochemical factors and oxidative stress parameters, such as TAC (total antioxidant capacity), MDA (Malondialdehyde), and the thiol group, were measured in these groups. Results: The means of TAC, MDA and UA (uric acid) in the hyperthyroid group were significantly higher than those in the hypothyroid group (P < 0.01, P < 0.01, P < 0.05) but the mean of thiol in the hypothyroid group was significantly higher than that of the hyperthyroid group (P < 0.01). Also, the mean of TAC level in the hypothyroid group was significantly lower than the control group (P < 0.05) and the mean of thiol group in the hypothyroid group was significantly higher than the control group (P < 0.05), but there was no other significant difference between the groups compared to the control group. Among the biochemical parameters, the mean LDH (lactate dehydrogenase) in the hyperthyroid group also showed a significant difference in comparison to the hypothyroid group (P < 0.05), but there was no significant difference between the groups in the mean of the other biochemical parameters. Conclusions: According to the results of this study, hypothyroidism and hyperthyroidism are associated with a disturbance in the oxidation state of the body, and the use of antioxidants can help improve these diseases, especially in hyperthyroidism, due to enhanced metabolism and production of more free radicals.

Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Male Smokers

2019 ◽  
Vol 22 (7) ◽  
pp. 496-501
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Parisa Falsafi ◽  
Hamidreza Abolsamadi ◽  
Mohammad T. Goodarzi ◽  
Jalal Poorolajal
Keyword(s):  
Oxidative Stress ◽  
Uric Acid ◽  
Free Radicals ◽  
Antioxidant Capacity ◽  
Cigarette Smoke ◽  
Total Antioxidant Capacity ◽  
The Body ◽  
Total Antioxidant ◽  
The Mean ◽  
And Oxidative Stress

Background: Cigarette smoke free radicals can cause cellular damage and different diseases. All the body fluids have antioxidants which protect against free radicals. Objective: The aim of this study was to evaluate salivary total antioxidant capacity and peroxidase, uric acid and malondialdehyde levels in smokers and a nonsmoking control group. Methods: Unstimulated saliva was collected from 510 males. A total of 259 subjects were current smokers and 251 were non-smokers. The levels of salivary total antioxidant capacity, uric acid, peroxidase and malondialdehyde were measured using standard procedures. Data were analyzed with t test and ANOVA. Results: The smokers were younger and dental hygiene index was higher than healthy nonsmoking controls. The mean total antioxidant capacity in smokers and nonsmokers was 0.13±0.07 and 0.21±011, respectively (P=0.001). Smokers had significantly lower peroxidase and uric acid levels than healthy controls. In addition, the mean malondialdehyde levels in the smokers and nonsmokers were 4.55 ±2.61 and 2.79 ±2.21, respectively (P=0.001). Conclusion: Cigarette smoke produces free radical and oxidative stress, causing many side effects. Salivary antioxidant levels decreased and malondialdehyde levels increased in smokers, indicating the high oxidative stress among smokers compared to nonsmokers. Cigarette smoke had deleterious effects on main salivary antioxidants levels.

Analysis of the breeding records of a herd of pigs

1954 ◽  
Vol 45 (1) ◽  
pp. 19-27 ◽  
Author(s):  
R. Braude ◽  
P. M. Clarke ◽  
K. G. Mitchell
Keyword(s):  
Statistical Analysis ◽  
Large White ◽  
Seasonal Differences ◽  
Significant Difference ◽  
The Mean ◽  
The Difference ◽  
Length Of Gestation

1. A survey and. statistical analysis of the records of litters born in the N.I.R.D. herd of Large White pigs over a period of 16 years are presented.2. The mean length of gestation was 114 days for both gilts and sows.3. The mean numbers of pigs born alive and born dead per litter from gilts, 10·0 and 0·2 respectively, were both significantly smaller than the corresponding figures for sows, 11·8 and O·8. No seasonal differences were found.4. The mean total losses of pigs from birth to weaning at 8 weeks were 29·5% of pigs born alive. The losses in litters from gilts, 23·0%, were significantly smaller than in litters from sows, 31·0%. The losses during the winter months both for gilts, 32·5%, and for sows, 35·9%, were significantly higher than during the summer months, 19·4% and 26·7% for gilts and sows respectively.5. Over 50% of the total losses were due to crushing by the dam, 74·1% of the losses from this cause occurring during the first 48 hr. after birth.6. The mean number of pigs weaned per litter was 8·0. The difference between the number weaned per litter from gilts, 7·7, and per litter from number of pigs was weaned per litter from sows during the summer months, 8·7, than during the winter months, 7·4.7. There was no significant difference between the number of male and of female pigs weaned.8. The mean weight at weaning was 29·9 lb., male pigs, 30·3 lb., being significantly heavier than females, 29·51b. Pigs in litters from gilts, 27·81b., were significantly lighter at weaning than those from sows, 30·0 lb., and for both gilts and sows, pigs reared during the summer months, 29·7 and 31·8 lb. respectively, were significantly heavier than those reared during the winter months, 25·9 and 28·2 lb. respectively.

Exposure to bifenthrin causes immunotoxicity and oxidative stress in male mice

2012 ◽  
Vol 29 (9) ◽  
pp. 991-999 ◽  
Author(s):  
Yuanxiang Jin ◽  
Xiuhong Pan ◽  
Zhengwei Fu
Keyword(s):  
Oxidative Stress ◽  
Male Mice ◽  
And Oxidative Stress

scholarly journals Effect of Acupressure on Stress and Anxiety of Patients with Multiple Sclerosis: A Sham-controlled Randomized Clinical Trial

2020 ◽  
Vol 10 (3) ◽  
pp. 270-283
Author(s):  
Mohammad Ali Nahayati ◽  
◽  
Seyed Abolfazl Vaghar Seyyedin ◽  
Hamid Reza Bahrami-Taghanki ◽  
Zahra Rezaee ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Test Scores ◽  
Sham Group ◽  
Applied Pressure ◽  
Intervention Group ◽  
T Test ◽  
Significant Difference ◽  
The Mean

Objective: Stress and anxiety are common in patients with Multiple Sclerosis (MS). These complications exacerbate the symptoms of MS. This study aims to evaluate the effect of acupressure on stress and anxiety of MS patients. Methods: In this randomized clinical trial, participants were 106 MS Patients referred to Multiple Sclerosis Society in Mashhad, Iran. After signing an informed consent form, they completed a demographic form and stress, and the Depression Anxiety Stress Scale - 42 items (DASS-42). Then, they were randomly assigned into intervention and sham groups by tossing a coin. Participants in the intervention group were asked apply a pressure on the Shenmen and Yin Tang acupoints for one month (once per day for 15 minutes), while the sham group applied pressure 2.5 cm below the Shenmen acupoint and 3 cm above the Yin Tang acupoint. Participants in each group were then completed the DASS-42 again one hour after the final session. Data were analyzed using chi-square test, Kolmogorov-Smirnov test, independent t-test, and Paired t-test. Results: In the intervention and sham groups, there was no statistically significant difference between the mean pre-test scores of stress (34.73±5.80 vs. 33.06±6.42) and anxiety (27.09±6.99 vs. 25.31±6.88), and neither between the mean post-test scores of stress (29.20±6.21 vs. 33.73±9.44) and anxiety (22.79±5.68 vs. 25.21±6.72) (P>0.05). However, comparison of DASS-42 scores between groups showed that the mean scores of stress and anxiety in the intervention group were significantly lower than in the sham group (P< 0.05). Conclusion: It seems that acupressure can be used along with other therapeutic and pharmacological interventions to reduce stress and anxiety in patients with MS.

scholarly journals Haemangiopericytomas in dogs: Epidemiological analysis of 136 cases

2019 ◽  
Vol 75 (01) ◽  
pp. 6178-2019
Author(s):  
RAFAŁ SAPIERZYŃSKI ◽  
MICHAŁ CZOPOWICZ ◽  
DIANA STOPKA
Keyword(s):  
Statistical Analysis ◽  
Entire Body ◽  
Frequency Of Occurrence ◽  
Epidemiological Analysis ◽  
Significant Difference ◽  
The Mean ◽  
Purebred Dogs

Perivascular wall tumors (PWTs or haemangiopericytomas) are common in dogs, and they are clinically important in veterinary oncology, but there is no data on the occurrence of these tumors in dogs in Poland. The aim of the present study was an epidemiological analysis of canine perivascular wall tumors. The study included 136 dogs with recognized PWTs (whose frequency of occurrence was the same in females and males). The mean age of the dogs was 9.8±3.0 years, with no statistically significant difference between sexes. Among the dogs, 40.7% were mongrels, and 59.3% were purebred. Among the purebred dogs, 21 (26.3%) were German Shepherds, 17 (21.3%) were Boxers, 5 (6.3%) were Doberman pinchers and Rottweilers. Statistical analysis revealed that four breeds were predisposed to perivascular wall tumors in comparison to the general dog population: German Shepherds, Boxers, Doberman pinchers and Rottweilers. Moreover, large-breed (but not giant-breed) dogs were shown to be predisposed to PWTs. Tumors were located in the skin or subcutis of the entire body, mostly on the legs, more seldom on the trunk or the head. The most typical location for PWTs was the elbow area (11% of all cases).

scholarly journals The Effect of Herniarin on Spatial Working Memory, Pain Threshold, and Oxidative Stress in Ischemic Hypoperfusion Model in Rats

2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Zahra Nazari Barchestani ◽  
◽  
Maryam Rafieirad ◽  
Keyword(s):  
Oxidative Stress ◽  
Pain Threshold ◽  
Male Rats ◽  
Control Group ◽  
Tail Flick ◽  
Pain Thresholds ◽  
Ischemic Group ◽  
Significant Difference ◽  
The Brain ◽  
And Oxidative Stress

Background: Ischemia causes severe neuronal damage and induces oxidative stress, memory impairment, and reduces pain threshold. Herniarin is a powerful antioxidant. Objectives: This study aimed to evaluate the effect of herniarin on memory, pain, and oxidative stress in an ischemia model in male rats. Materials & Methods: In this study, 50 male rats were divided into 5 groups of control, sham, ischemic, and two other ischemic groups, which received herniarin at doses of 150 and 300 mg/kg by gavage for 14 days. Behavioral tests were performed by shuttle box, and Y-maze and pain tests were performed by Tail-Flick test. Then, the rats’ brains were extracted to evaluate lipid peroxidation and measure the levels of thiol and Glutathione Peroxidase (GPX) in the hippocampus and striatum tissues. The results were expressed as Mean±SEM and then analyzed using suitable statistical methods of ANOVA and least significant difference post-hoc test in SPSS V. 20. Results: Herniarin significantly increased the avoidance memory, spatial memory, and pain thresholds of ischemic rats at different concentrations (P<0.001). Besides, the amount of malondialdehyde (MDA) and thiol in the ischemic group increased significantly in comparison to the control group (P<0.001). Also, in the ischemic group, GPX (P<0.001) significantly decreased. Decreased MDA (P<0.001) and thiol (P<0.001) and increased GPX levels were observed with herniarin administration (P<0.01). Conclusion: According to this study’s results, herniarin can remove free radicals and oxidant substances from the brain. Thus, it improves memory and pain thresholds in the brain hypoperfusion ischemia model.

Comparison of the Stress Response of Listeria monocytogenes Strains with Sprout Colonization

2008 ◽  
Vol 71 (8) ◽  
pp. 1556-1562 ◽  
Author(s):  
LISA GORSKI ◽  
DENISE FLAHERTY ◽  
JESSICA M. DUHÉ
Keyword(s):  
Oxidative Stress ◽  
Statistical Analysis ◽  
Listeria Monocytogenes ◽  
Stress Response ◽  
Foodborne Pathogen ◽  
Acid Stress ◽  
Broccoli Sprouts ◽  
Alfalfa Sprouts ◽  
Different Strains ◽  
And Oxidative Stress

Twenty-nine strains of the foodborne pathogen Listeria monocytogenes were tested for their ability to colonize alfalfa, radish, and broccoli sprouts and their capacity to withstand acid and oxidative stress, two stresses common to the sprouting environment. Wide variation in the ability of different strains to colonize alfalfa sprouts were confirmed, but the variations among radish and broccoli sprouts were not as large. With a few exceptions, strains that were poor colonizers of alfalfa tended to be among the poorer colonizers of radish and broccoli and vice versa. The strains also were variable in their resistance to both acid and oxidative stress. Statistical analysis revealed no correlation between acid stress and sprout colonization, but there was a positive correlation between resistance to oxidative stress and colonization of all three sprout types. Although the response to oxidative stress is important for L. monocytogenes virulence, it also may be important for life outside of a host.

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