scholarly journals Effect of different periodontal ligament simulating materials on the incidence of dentinal cracks during root canal preparation

2018 ◽  
Vol 12 (3) ◽  
pp. 196-200
Author(s):  
Ajita Rathi ◽  
Prateeksha Chowdhry ◽  
Mamta Kaushik ◽  
Pallavi Reddy ◽  
Roshni Roshni ◽  
...  
Keyword(s):  
Root Canal ◽  
Periodontal Ligament ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Root Canal Preparation ◽  
Impression Material ◽  
Group 1

Background. The present study was undertaken to evaluate the incidence of dentinal cracks during root canal preparation with different periodontal ligament simulating materials in vitro. Methods. Seventy freshly extracted human mandibular first premolars were selected and divided into 7 groups in terms of simulating material: group 1: polyether impression material; group 2: polyvinyl acetate adhesive; group 3: polyvinyl siloxane impression material; group 4: cyanoacrylate adhesive; group 5: epoxy resin adhesive; group 6: positive control, without any periodontal ligament simulation; and group 7: negative control, where neither a periodontal ligament simulating material was used nor canal preparation was carried out. Root canal preparation was carried out in all the groups followed by sectioning of roots at 3 mm, 6 mm and 9 mm. The sections were evaluated under a stereomicroscope at ×2.5 for the presence or absence of cracks. Chi-squared test was used to compare the appearance of defective roots between the different experimental groups. Results. The least number of cracks were found in the negative control group, followed by group 1 where polyether impression material was used for periodontal ligament simulation. The difference was significant with a P-value of 0.002 for coronal sections. Conclusion. Under the limitation of the present study, polyether and polyvinyl siloxane (light body) can both be used for simulation of periodontal ligament.

scholarly journals Diffusion of calcium hydroxide through dentinal tubules of retreated root canals: An in vitro study

2018 ◽  
Vol 12 (03) ◽  
pp. 386-392 ◽  
Author(s):  
Mohammed Mustafa ◽  
Wafa Hassan Alaajam ◽  
Ahmed Abdul Azeim ◽  
Najla Aedh Alfayi ◽  
Rahaif Misfer Alqobty ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Normal Saline ◽  
Root Canal ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Group I ◽  
Group Ii

ABSTRACTObjective: Studies about the dispersion of hydroxyl ions through dentin of endodontically retreated teeth are scarce. The present study aims at examining the diffusion of calcium hydroxide in vitro by recording the pH changes of retreated root canal using two types of calcium hydroxide preparations. Materials and Methods: A total of 45 recently extracted single-rooted mandibular first premolars were collected and cleaned from calculus and remains of periodontal tissue. The teeth so collected were stored in thymol 10% solution till use. During collection, teeth with cracks, open apices, and restorations were excluded. Root canal preparations were performed using Protaper rotary system files, till #F3 file. All canals were obturated using cold lateral condensation and zinc oxide-based sealer. After 7 days, all gutta-percha were removed and the canals were irrigated with normal saline. The teeth were then randomly distributed into three groups. Canals in Group I were kept empty without any dressing to serve as a negative control group, Canals in Group II were filled with a freshly prepared mixture of calcium hydroxide powder with normal saline solution, and Group III was filled with Metapex. Periodically, the calcium hydroxide material was removed and the pH was measured using pH meter at 7, 10, 14, and 30 days. Results: The pH values of Group II and III ranged between 9.2 and 11.2. This was found to be highly significant against the negative control group. After 7 and 10 days, freshly mixed Ca(OH)2 showed the higher pH than the creamy Metapex, and statistically the difference was highly significant after 10 days (P<0.001). After 14 days, Metapex group pH was higher than freshly mixed Ca(OH)2, but it was not statistically significant as they reached nearly the same pH after 30 days (P>0.05). Conclusion: All Ca(OH)2 preparations had high pH around the roots after 7 and 10 days. Metapex continued to have higher pH after 14 days.

scholarly journals Antibacterial Effects of a 940 nm Diode Laser With/ Without Silver Nanoparticles Against Enterococcus faecalis

2021 ◽  
Vol 12 (1) ◽  
pp. e73-e73
Author(s):  
Seyedeh Sareh Hendi ◽  
Maryam Shiri ◽  
Banafsheh Poormoradi ◽  
Mohammad Yousef Alikhani ◽  
Saeid Afshar ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Diode Laser ◽  
Sodium Hypochlorite ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Antibacterial Effects ◽  
Group 4 ◽  
Group 1

Introduction: The final goal of root canal therapy is to remove of the most bacteria from the root canal. This study aimed at comparing the antibacterial effects of a diode laser with a wavelength of 940nm and silver nanoparticles and the synergic effects of both techniques on Enterococcus faecalis. Methods: Ninety single-rooted human teeth were decoronated and prepared with rotary files. The samples were irrigated with sodium hypochlorite and EDTA17%. Then they were autoclaved and contaminated with E. faecalis suspension (1.5 × 108 CFU/mL) for 21 days. The samples were fixed in a microtube and were randomly divided into 4 experimental (n=20) groups and a negative control group (n=10) as follows: Group 1: hypochlorite sodium 5%, Group 2: silver nanoparticle, Group 3: diode laser, and Group 4: diode laser and silver nanoparticle. The samples were obtained from dentin chips before and after the intervention. The data were analysed using the Kruskal-Wallis nonparametric test. Furthermore, alterations in bacterial colonies were entered using the Wilcoxon signed ranks test (α=0.05). Results: There was a significant decrease in colony counts for all groups after interventions (P value<0.05). Also, all groups showed more reductions in colony counts compared with the negative control group (P value <0.004). There was a significant reduction for group 1 in comparison with other groups (P value <0.001) and this group had an extreme decrease of colony counts (RCC=100%). There was an important differential between silver nanoparticles and diode laser groups in bacterial counts (P value<0.001) and silver nanoparticles (RCC=83.15%) had more efficiency than the diode laser (RCC=41/33%). RCC of group 4 was 68/52%. Conclusion: Followed by sodium hypochlorite 5%, silver nanoparticles were the most effective antibacterial substances. The 940 nm laser diode had less antibacterial effect compared to its use with silver nanoparticles.

Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

2019 ◽  
Vol 20 (1) ◽  
pp. 12-18
Author(s):  
Sameh El-Nabtity
Keyword(s):  
New Zealand ◽  
Intramuscular Injection ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Aqueous Extracts ◽  
Blood And Urine Samples ◽  
Group 2 ◽  
New Zealand Rabbits ◽  
Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

scholarly journals In vitro comparison of the cytotoxicity of different endodontic sealers; AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal

2021 ◽  
Author(s):  
Mehdi Dastorani ◽  
Muhammad javad Aliee ◽  
Raheleh Halabian ◽  
Mostafa Solati ◽  
Mohammadsadegh Alemrajabi
Keyword(s):  
Repeated Measures ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Periapical Lesions ◽  
Ah Plus ◽  
Low Cytotoxicity ◽  
Endodontic Sealers

Abstract Background: This study aimed to assess the cytotoxicity of four commonly used endodontic sealers namely AH Plus, AdSeal, Endoseal MTA, and GuttaFlow Bioseal against human gingival fibroblasts (HGFs). Methods: After culturing the HGFs, they were exposed to the respective sealers in set form and in five different weights, after sterilization. The cytotoxicity of the sealers was evaluated after 1, 3 and 7 days using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed by repeated measures ANOVA. Results: After 24 h, all sealers showed low cytotoxicity. However, all sealers in 250 mg and 500 mg weights showed significantly higher cytotoxicity than the negative control group at 72 h, and 7 days (P<0.05) except for AdSeal in 80 mg weight (P>0.05). AH Plus was significantly more cytotoxic than other sealers at 3 and 7 days (P<0.05) while AdSeal had the closest results to the negative control group, and showed significantly higher biocompatibility than other sealers in 250 mg concentration. Conclusion: AdSeal showed the highest biocompatibility while AH Plus had the highest cytotoxicity among the tested sealers. Thus, its application may delay the healing of periapical lesions.

scholarly journals Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal wall: An in-vitro study

2017 ◽  
Vol 90 (3) ◽  
pp. 327-332 ◽  
Author(s):  
Deepak Singh Kirar ◽  
Pradeep Jain ◽  
Pallav Patni
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Positive Control ◽  
Positive Control Group ◽  
Control Group ◽  
Passive Ultrasonic Irrigation ◽  
Ultrasonic Irrigation ◽  
Group 2 ◽  
Group 1

Background and aim: Comparison of different irrigation and agitation methods for the removal of two types of calcium hydroxide medicaments from the root canal walls.Methods: Fifty extracted single rooted teeth were selected for this study. After decoronation, the root canals of these teeth were prepared to the size F3 (30 no.) using rotary ProTaper file system. These samples were randomly divided into four groups. Group 1 (n=20) were filled completely with water based calcium hydroxide (CH), Group 2 (n=20) were filled with oil based CH using lentulo spiral, Group 3 (n=5) - the positive control group received the CH as intracanal medication, but no subsequent removal, Group 4 (n=5) - the negative control did not receive CH placement. Further on, Group 1 and Group 2 were divided into four sub-groups (n=5). In sub-group A we performed conventional syringe irrigation with side-vented needle sub-group B) manual dynamic agitation, sub-group C sonic agitation using endoactivator, sub-group D passive ultrasonic irrigation (PUI). Roots were split longitudinally into mesial and distal halves. Digital images of the root canal walls were acquired by a Dental Operating Microscope (DOM) and assessed by using a scoring criteria at different thirds (coronal, middle and apical) of the root canal as follows: score 1, score 2, score 3, and score 4. Data were analyzed applying one-way analysis of variance (ANOVA) and Tukey’s multiple comparison tests at a 95% confidence interval (P < 0.05).Results: Statistically significant differences were not found between the experimental groups and the negative group in any one third of the root canal (P>0.05). However, a difference did exist between the experimental groups and the positive control group (P<0.05). None of the experimental groups totally removed CH substances from root canal walls.Conclusion: Among all experimental groups, removal of CH was best achieved by sonic agitation using endoactivator followed by passive ultrasonic irrigation (PUI), manual dynamic agitation and conventional syringe irrigation with side-vented needle.

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals Effect of Different Irrigation Solutions on the Diffusion of MTA Cement into the Root Canal Dentin

Materials ◽  
2020 ◽  
Vol 13 (23) ◽  
pp. 5472
Author(s):  
José Pedro Martinho ◽  
Sara França ◽  
Siri Paulo ◽  
Anabela Baptista Paula ◽  
Ana Sofia Coelho ◽  
...  
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Treatment Success ◽  
Confocal Laser ◽  
Control Group ◽  
Middle Section ◽  
Group 2 ◽  
Root Canal Dentin ◽  
Group 1

(1) Aim: This study aims to analyze the in vitro infiltration of a silicate root canal sealer into dentinal tubules after using different endodontic irrigating solutions. (2) Methods: Twenty-nine teeth with single roots were separated into three groups according to the final irrigation protocol: G1 n = 10) = 17% EDTA (ethylenediaminetetraacetic acid) + 3.0% sodium hypochlorite (NaOCl), G2 (n = 10) = 17% EDTA + 2.0% chlorhexidine and G3 (Control group, n = 9) = 17% EDTA + saline solution. Root canals were filled using cold lateral compaction technique with MTA Fillapex sealer and gutta-percha. The sealer was labeled with rhodamine B. The teeth were segmented at the middle and third apical sections, which were visualized using 10× confocal laser microscopy to determine the sealer penetration percentage. (3) Results: In the apical section, no statistically significant differences were found between the groups regarding sealer penetration. In the middle section, Group 1 obtained the highest percentage, and Group 2 the lowest (p = 0.004). Group 1 also presented statistically significant differences in the Control Group (p = 0.031) and had close sealer penetration values. Meanwhile, the Control Group (p = 0.023) and Group 2 (p = 0.029) revealed a significant decrease of sealer penetration between the apical and middle sections. (4) Conclusion: The obtained results support that final irrigation with NaOCl promoted similar sealer penetration in the apical and middle sections. On the other hand, a significant decrease in the sealer penetration of the middle section was observed for the chlorhexidine and saline groups. Compared to other irrigant solutions, NaOCl promotes more uniform sealer penetration, which can correlate with better sealing and, consequently, higher endodontic treatment success.

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

Cytotoxicity and Hemolytic Properties of Nano-Hydroxyapatite/Polyetheretherketone Biocomposites

2016 ◽  
Vol 848 ◽  
pp. 567-572 ◽  
Author(s):  
Lin Wang ◽  
Shen Hua Song ◽  
Zheng Zhi Wu ◽  
Li Hong Duan ◽  
Chun Bao Wang
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
National Standard ◽  
Control Group ◽  
Culture Solution ◽  
Contact Method ◽  
Cytotoxicity Test ◽  
L929 Cells ◽  
Hemolysis Test

In order to evaluate the cytocompatibility and hemolytic properties of n-HA/PEEK biocomposites the nanohydroxyapatite/polyetheretherketone (n-HA/PEEK) biocomposites were successfully prepared. The mechanical properties of the biocomposites were proximal to human bone, at the same time, they had the optimal value with the HA volume content of 5%. The PEEK and n-HA/PEEK biocomposites with different HA content extraction medium was prepared with fresh medium. Simple DMEM culture solution was taken as negative control group. The pure PEEK and 5vol.%, 15vol.%, 30vol.% n-HA/PEEK biocomposites were the testing group. The relative proliferation rate of L929 cells was determined on the 1st, 2nd, 3rd and 6th days with CCK-8 assay. The cytotoxicity of n-HA/PEEK biocomposites were evaluated according to ISO 10993-5: 2009. The L929 cells morphology and growth on the 1st, 2nd, 3rd and 6th days were determined under inverted microscope. The hemolysis test in vitro of n-HA/PEEK biocomposites were evaluated through measuring erythrocyte lysis and ferro-hemoglobin freeing degree with indirect contact method basing on ISO 10993-4:2009. The experimental results showed that the growth and morphology of cells in pure PEEK and n-HA/PEEK biocomposites extraction medium had no difference from negative control group. Cytotoxicity test showed that PEEK and n-HA/PEEK biocomposites did not have obvious toxicity on L929 cells, and the cytotoxicity of these extracts was in grade 0-1. Hemolysis test suggested that PEEK and n-HA/PEEK biocomposites did not have obvious hemolysis reaction, and the hemolysis rate of PEEK and n-HA/PEEK biocomposites were 2.37%, 1.71%, 1.05% and 1.32% respectively, which are less than the national standard (5%). It may be concluded that the n-HA/PEEK biocomposites did not have obvious cytotoxicity and hemolysis reaction, which demonstrated that n-HA/PEEK biocomposites had good cytocompatibility.

scholarly journals Replacement of vitamin-mineral premix by Spirulina and its effect on the performance of broiler

2018 ◽  
Vol 2 ◽  
pp. 39
Author(s):  
Shahinur Nahar Moury ◽  
Md. Touhiduzzaman Sarker ◽  
Adhita Sri Prabakusuma ◽  
Md. Imrul Hasan Russel ◽  
Md. Shahidul Islam
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Group 4 ◽  
Group 6 ◽  
Group 5 ◽  
And Control ◽  
Group 1

The main purpose of this study was to explore the effects of Spirulina (Spirulina platensis) as a feed supplement by replacing Vitamin-Mineral Premix on the performance of broiler. The study explored that final body weight was 1039, 1070, 1044, 1065, 1117 and 893 g/bird in group 1, 2, 3, 4, 5 and 6 where significantly (p<0.5) higher weight observed in Spirulina group (5) but lower in negative control group (6). Feed intake observed more or less similar in all groups (1483±24g; p>0.5). Feed conversion ratio (kg FI/kg LWG) observed significantly different like 1.72, 1.65, 1.69, 1.62, 1.61 and 1.99 in group 1, 2, 3, 4, 5 and 6 respectively (p<0.5) where higher in negative group (6) and control group (1), but is lowest and similar in 75% Spirulina group (4) and highest (100%) Spirulina group (5). %Ash of different dietary groups were 46.35, 46.54, 48.93, 49.93, 55.07 and 46.92 of group 1, 2, 3, 4, 5 and 6 respectively. The %ash of bone was gradually increased among dietary groups and the best result was obtained in 100% Spirulina group 5 (55.07). It also revealed that diets containing no vitamin-mineral premix with 100% Spirulina improve the performance of broiler. The findings suggest that poultry farmer can use spirulina as a feed item for broiler for more yield.

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