scholarly journals Effect of alpha-lipoic acid on antioxidant gene expression and kidney injury in alloxan-induced diabetic rats

2018 ◽  
Vol 8 (1) ◽  
pp. 6-6 ◽  
Author(s):  
Parisa Jamor ◽  
Hassan Ahmadvand ◽  
Hesam Ashoory ◽  
Esmaeel Babaeenezhad
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Kidney Injury ◽  
Diabetic Rats ◽  
Pcr Analysis ◽  
Control Group ◽  
Diabetic Patients ◽  
Alpha Lipoic Acid ◽  
The Impact ◽  
Gpx Activity

Background: Myeloperoxidase (MPO) is involved in the initiation, progression, and complications of atherosclerosis in diabetic patients. Objectives: In the current study, the impact of alpha-lipoic acid (LA), a natural antioxidant and a cofactor in the enzyme complexes on MPO, catalase (CAT) and glutathione peroxidase (GPx) activity, glutathione (GSH) and malondialdehyde (MDA) level, histopathology of kidney and expression of antioxidant enzymes, superoxide dismutase (SOD), GPx and CAT which are involved in the detoxification of reactive oxygen species (ROS), was evaluated in alloxan-induced diabetic rats. Materials and Methods: In this study, 30 male Rattus norvegicus rats randomly divided into three groups; control (C), non-treated diabetic (NTD), and LA-treated diabetics (LATD) was induced by alloxan monohydrate (100mg/kg; subcutaneous [SC]). Then treatment was performed with alphaLA (100 mg/kg intraperitoneal (i.p) daily to 6 weeks). Blood sample of animals collected to measure levels of MPO, CAT and GPx activity GSH and MDA. Kidney paraffin sections were prepared to estimate histological studies and to measure quantitative gene expression SOD, GPX and CAT in kidney. Results: Induction of diabetes led to a significant increase in MPO and MDA, reduced GSH level and GPx and CAT activities (P < 0.05). However, treatment with alpha-LA led to a significant elevation in GPx, CAT and GSH levels with a reduction in MPO activities and MDA levels (P < 0.05). Furthermore, the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis results showed increased expressions of GPx, CAT and SOD enzyme in the treatment group compared with the diabetic control group. Histopathological lesions such as increased glomerular volume and lymphocyte infiltration were attenuated in the alpha-LA treated group. Conclusions: Our findings indicated that alpha-LA supplementation is effective in preventing complications induced by oxidative stress and atherosclerosis in diabetic rats.

scholarly journals Endurance Training Regulates Expression of Some Angiogenesis-Related Genes in Cardiac Tissue of Experimentally Induced Diabetic Rats

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 498
Author(s):  
Mojdeh Khajehlandi ◽  
Lotfali Bolboli ◽  
Marefat Siahkuhian ◽  
Mohammad Rami ◽  
Mohammadreza Tabandeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endurance Training ◽  
Molecular Mechanisms ◽  
Cardiac Tissue ◽  
Critical Role ◽  
Diabetic Rats ◽  
Moderate Intensity ◽  
Pcr Analysis ◽  
Cardiac Tissues ◽  
The Impact

Exercise can ameliorate cardiovascular dysfunctions in the diabetes condition, but its precise molecular mechanisms have not been entirely understood. The aim of the present study was to determine the impact of endurance training on expression of angiogenesis-related genes in cardiac tissue of diabetic rats. Thirty adults male Wistar rats were randomly divided into three groups (N = 10) including diabetic training (DT), sedentary diabetes (SD), and sedentary healthy (SH), in which diabetes was induced by a single dose of streptozotocin (50 mg/kg). Endurance training (ET) with moderate-intensity was performed on a motorized treadmill for six weeks. Training duration and treadmill speed were increased during five weeks, but they were kept constant at the final week, and slope was zero at all stages. Real-time polymerase chain reaction (RT-PCR) analysis was used to measure the expression of myocyte enhancer factor-2C (MEF2C), histone deacetylase-4 (HDAC4) and Calmodulin-dependent protein kinase II (CaMKII) in cardiac tissues of the rats. Our results demonstrated that six weeks of ET increased gene expression of MEF2C significantly (p < 0.05), and caused a significant reduction in HDAC4 and CaMKII gene expression in the DT rats compared to the SD rats (p < 0.05). We concluded that moderate-intensity ET could play a critical role in ameliorating cardiovascular dysfunction in a diabetes condition by regulating the expression of some angiogenesis-related genes in cardiac tissues.

scholarly journals DecreasedO-GlcNAcylation of the key proteins in kinase and redox signalling pathways is a novel mechanism of the beneficial effect of α-lipoic acid in diabetic liver

2013 ◽  
Vol 110 (3) ◽  
pp. 401-412 ◽  
Author(s):  
Svetlana Dinić ◽  
Jelena Arambašić ◽  
Mirjana Mihailović ◽  
Aleksandra Uskoković ◽  
Nevena Grdović ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Lipoic Acid ◽  
Oxidant Stress ◽  
Sulfhydryl Group ◽  
Diabetic Rats ◽  
Pcr Analysis ◽  
Transcriptional Level ◽  
Thiobarbituric Acid Reactive Substance ◽  
Physiological Level

The present study aimed to investigate the effects of the treatment with α-lipoic acid (LA), a naturally occurring compound possessing antioxidant activity, on liver oxidant stress in a rat model of streptozotocin (STZ)-induced diabetes by examining potential mechanistic points that influence changes in the expression of antioxidant enzymes such as catalase (CAT) and CuZn/Mn superoxide dismutase(s) (SOD). LA was administered for 4 weeks by daily intraperitoneal injections (10 mg/kg) to STZ-induced diabetic rats, starting from the last STZ treatment. LA administration practically normalised the activities of the indicators of hepatocellular injury, alanine and aspartate aminotransferases, and lowered oxidative stress, as observed by the thiobarbituric acid-reactive substance assay, restored the reduced glutathione:glutathione disulphide ratio and increased the protein sulfhydryl group content. The lower level of DNA damage detected by the comet assay revealed that LA reduced cytotoxic signalling, exerting a hepatoprotective effect. The LA-treated diabetic rats displayed restored specific enzymatic activities of CAT, CuZnSOD and MnSOD. Quantitative real-time PCR analysis showed that LA restoredCATgene expression to its physiological level and increasedCuZnSODgene expression, but the gene expression ofMnSODremained at the diabetic level. Although the amounts of CAT and CuZnSOD protein expression returned to the control levels, the protein expression of MnSOD was elevated. These results suggested that LA administration affected CAT and CuZnSOD expression mainly at the transcriptional level, and MnSOD expression at the post-transcriptional level. The observed LA-promoted decrease in theO-GlcNAcylation of extracellular signal-regulated kinase, protein 38 kinase, NF-κB, CCAAT/enhancer-binding protein and the antioxidative enzymes themselves in diabetic rats suggests that the regulatory mechanisms that supported the changes in antioxidative enzyme expression were also influenced by post-translational mechanisms.

scholarly journals The effect of Alpha-lipoic acid in glyphosate treated granulosa cells on the human folliculogenesis genes

2021 ◽  
Author(s):  
Sakineh Kaboli Kafshgiri ◽  
Armin Attaranzadeh ◽  
Fahimeh Ghasemi ◽  
Ebrahim miri-moghaddam
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Granulosa Cells ◽  
Morphological Structure ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Culture Cells ◽  
Wide Range ◽  
Male Patients ◽  
Star Gene

Abstract Pesticides have a wide range of infertility in female reproductive. GLP is the most common herbicide used worldwide. The present study tended to evaluate the effects of alpha-lipoic acid (ALA) on expression of folliculogenesis genes in human granulosa cells (GCs) treated with glyphosate. In this study, GC samples were taken from infertile male patients who had tubal factors and received ICSI or IVF treatment for the first time. The medium used to culture cells was DMEM-F12 containing FBS 10%, penicillin 1%, and streptomycin 1%. The cells were treated with glyphosate (GLP) (250 µg/ml), ALA (50 µg/ml), and their mixture (250 GLP + 50 ALA) for 24 hr. The FoxO1, NOX4, Vnn1, and STAR gene expression in GCs was determined by real-time PCR. Exposure to GLP decreased gene expression Vnn1 and STAR in the GCs, while in comparison to control group, FOXO1 and NOX4 expression insignificantly increased. Further, ALA treatment decreased FOXO1, NOX4, in the GCs, and increased VNN1 and STAR gene expression. Transcriptional expression of FoxO1, NOX4 and STAR in GCs also decreased in their combinations (GLY + ALA). At the same time, the level of Vnn1 mRNA in GCs was increased. The findings showed that exposure to GLP alters the morphological structure of GCs and the expression of follicogenic genes, leading to dysfunction of the reproductive system. Moreover, the results showed that cell characteristics were preserved efficiently in human GCs exposed to ALA as an antioxidant.

scholarly journals Endurance Training Regulates Expression of Some Angiogenesis-Related Genes in Cardiac Tissue of Experimentally Induced Diabetic Rats

2021 ◽  
Author(s):  
Mojdeh Khajehlandi ◽  
Lotfali Bolboli ◽  
Marefat Siahkuhian ◽  
Mohammad Rami ◽  
Mohammadreza Tabandeh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endurance Training ◽  
Molecular Mechanisms ◽  
Cardiac Tissue ◽  
Critical Role ◽  
Diabetic Rats ◽  
Moderate Intensity ◽  
Pcr Analysis ◽  
Cardiac Tissues ◽  
The Impact

Exercise can ameliorate cardiovascular dysfunctions in diabetes condition, but its precise molecular mechanisms have not been entirely understood. The aim of the present study was to determine the impact of endurance training on expression of angiogenesis-related genes in cardiac tissue of diabetic rats. Thirty adults male Wistar rats were randomly divided into three groups (N=10) including diabetic training (DT), sedentary diabetes (SD), and sedentary healthy (SH) in which diabetes was induced by a single dose of streptozotocin (30 mg/kg). Endurance training (ET) with moderate-intensity was performed on a motorized treadmill for six weeks. Training duration and treadmill speed were increased during five weeks, but they were kept constant at the final week and slope was zero at all stages. Real-time polymerase chain reaction (RT- PCR) analysis was used to measure the expression of myocyte enhancer factor-2C (MEF2C), histone deacetylase-4 (HDAC4) and Calmodulin-dependent protein kinase II (CaMKII) in cardiac tissues of the rats. Our results demonstrated that six weeks of ET increased gene expression of MEF2C significantly (P&lt;0.05), and caused a significant reduction in HDAC4 and CaMKII gene expression in the DT rats compared to the SD rats (P&lt;0.05). We concluded that moderate-intensity ET could play a critical role in ameliorating cardiovascular dysfunction in a diabetes condition by regulating the expression of some angiogenesis-related genes in cardiac tissues.

scholarly journals The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1332
Author(s):  
Gilda M. Iova ◽  
Horia Calniceanu ◽  
Adelina Popa ◽  
Camelia A. Szuhanek ◽  
Olivia Marcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Rats ◽  
Gingival Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
The Impact ◽  
Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

scholarly journals Effects of Vitrification on the Blastocyst Gene Expression Profile in a Porcine Model

2021 ◽  
Vol 22 (3) ◽  
pp. 1222
Author(s):  
Cristina Cuello ◽  
Cristina A. Martinez ◽  
Josep M. Cambra ◽  
Inmaculada Parrilla ◽  
Heriberto Rodriguez-Martinez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Analysis ◽  
Survival Rates ◽  
Control Group ◽  
P Value ◽  
Transcriptome Profile ◽  
Embryo Viability ◽  
The Impact

This study was designed to investigate the impact of vitrification on the transcriptome profile of blastocysts using a porcine (Sus scrofa) model and a microarray approach. Blastocysts were collected from weaned sows (n = 13). A total of 60 blastocysts were vitrified (treatment group). After warming, vitrified embryos were cultured in vitro for 24 h. Non-vitrified blastocysts (n = 40) were used as controls. After the in vitro culture period, the embryo viability was morphologically assessed. A total of 30 viable embryos per group (three pools of 10 from 4 different donors each) were subjected to gene expression analysis. A fold change cut-off of ±1.5 and a restrictive threshold at p-value < 0.05 were used to distinguish differentially expressed genes (DEGs). The survival rates of vitrified/warmed blastocysts were similar to those of the control (nearly 100%, n.s.). A total of 205 (112 upregulated and 93 downregulated) were identified in the vitrified blastocysts compared to the control group. The vitrification/warming impact was moderate, and it was mainly related to the pathways of cell cycle, cellular senescence, gap junction, and signaling for TFGβ, p53, Fox, and MAPK. In conclusion, vitrification modified the transcriptome of in vivo-derived porcine blastocysts, resulting in minor gene expression changes.

Long-Term Alpha-Lipoic Acid (ALA) Antioxidant Therapy Reduces Damage in the Cardiovascular System of Streptozotocin-Induced Diabetic Rats

2021 ◽  
pp. 1-15
Author(s):  
Cristiane Simões Coelho Britto Ramos ◽  
Vivian Alves Pereira da Silva ◽  
Lanna Beatriz Neves Silva Corrêa ◽  
Renato de Souza Abboud ◽  
Gilson Teles Boaventura ◽  
...  
Keyword(s):  
Cardiovascular System ◽  
Lipoic Acid ◽  
Diabetic Rats ◽  
Antioxidant Therapy ◽  
Alpha Lipoic Acid

scholarly journals Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process

2021 ◽  
Vol 55 (4) ◽  
pp. 246
Author(s):  
Gede Wira Buanayuda ◽  
Hamdani Lunardhi ◽  
Indra Gusti Mansur
Keyword(s):  
Lipoic Acid ◽  
Intrauterine Insemination ◽  
Control Group ◽  
Infertile Couple ◽  
Preparation Process ◽  
Alpha Lipoic Acid ◽  
Sperm Preparation ◽  
Progressive Motility ◽  
Child Hospital

Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a control group, (k2) sperm preparation group without ALA, (k3) group of sperm preparation with the addition of ALA. The motility of spermatozoa was observed with the WHO 1999 method for 4 hours in units of percent. Progressive motility in k3 (47.95 ± 3.617) was higher than in k2 (38.05 ± 3.278) statistically significantly different after 3 hours of observation (p<0.0001). Progressive motility in k3 (78.8 ± 5.841) was higher than k1 (56.55 ± 7.511) from the initial observation (p <0.0001). The progressive motility of k2 (76.05 ± 6.768) was higher than k1 (56.55 ± 7.511) from the start of the observation (0.0001). It can be concluded that the addition of ALA in the sperm preparation process increases and maintains progressive motility that is better than sperm preparation without ALA addition after 3 hours of observation.

scholarly journals Diabetes mellitus increased integrins gene expression in rat endometrium at the time of embryo implantation

2019 ◽  
Author(s):  
Abbas Bakhteyari ◽  
Yasaman Zarrin ◽  
Parvaneh Nikpour ◽  
Zeinab Sadat Hosseiny ◽  
Zeinab Sadat Hosseiny ◽  
...  
Keyword(s):  
Gene Expression ◽  
Diabetes Mellitus ◽  
Group Treatment ◽  
Embryo Implantation ◽  
Treated Group ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Genes Expression ◽  
Normal Menstrual Cycle

Background: Diabetes mellitus deeply changes the genes expression of integrin (Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition? Objective: The aim of the current study was to investigate the endometrial gene expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time of embryo implantation. Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Real-time PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1, and β3 genes in rat’s endometrium. Results: The expression of all Itg subunits increased significantly in diabetic rats’ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on α3 and α4 and elevated these two subunits gene expression. Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure.

Alpha-lipoic acid prevents arsenic toxicity in HepG2 cells by upregulation of GSH content, metabolic activity and HOX1 gene expression

2007 ◽  
Vol 172 ◽  
pp. S47-S48
Author(s):  
Selene Huerta-Olvera ◽  
José Macías-Barragán ◽  
Juan Armendáriz-Borunda ◽  
Fernando Diaz-Barriga ◽  
Fernando Siller-López
Keyword(s):  
Gene Expression ◽  
Metabolic Activity ◽  
Lipoic Acid ◽  
Hepg2 Cells ◽  
Arsenic Toxicity ◽  
Alpha Lipoic Acid
Sign in / Sign up

Export Citation Format

Share Document