scholarly journals Comparison of the Antimicrobial Efficacy of Calcium Hydroxide and Photodynamic Therapy Against Enterococcus faecalis and Candida albicans in Teeth With Periapical Lesions; An In Vivo Study

2017 ◽  
Vol 8 (2) ◽  
pp. 72-78 ◽  
Author(s):  
Zohre Ahangari ◽  
Maryam Mojtahed Bidabadi ◽  
Mohammad Asnaashari ◽  
Afsaneh Rahmati ◽  
Fahimeh Sadat Tabatabaei
Keyword(s):  
Candida Albicans ◽  
Photodynamic Therapy ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
In Vivo Study ◽  
Antimicrobial Efficacy ◽  
Periapical Lesions

scholarly journals Antimicrobial Efficacy of Mineral Trioxide Aggregate against Therapy Resistant Endodontic Microorganisms

2016 ◽  
Vol 33 (3) ◽  
pp. 140-145
Author(s):  
Mst Laila Akter Banu ◽  
AKM Bashar ◽  
Md Mujibur Rahman Howlader ◽  
Md Shamsul Alam ◽  
Md Ashraf Hussain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zone Of Inhibition

Microorganisms, usually from the dental caries, are the main sources of diseases in dental pulp (root canals) and periapical region. Facultative bacteria and fungi have been identified in therapy resistant persistent endodontic infection. The objectives of this study was to evaluate the antimicrobial efficacy of Mineral Tri Oxide Aggregate (MTA) against therapy resistant endodontic microorganisms. The efficacy of MTA was also compared with that of calcium hydroxide. Six standard bacterial stains were used: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, bacillus subtilis, Candida albicans and Enterococcus faecalis. The agar diffusion method on Muller- Hilton media was employed. The plates containing media were inoculated with the specified bacterial suspensions. Two standard holes were prepared on each microorganism inoculated plate with a copper puncher and one hole was completely filled with MTA & the other with Ca (OH)2 . The plates were then kept at environmental temperature for one hour to ensure prediffusion and then incubated at 370C for 24 hours. After 24 hours, the diameters of inhibition zones were measured. Tests were replicated for thirty times for each sample and mean values were taken. Zone of inhibition as measured for MTA and Ca (OH)2 were statistically analyzed with Student’s t-Test and Post Hoc Games Howell Test and were presented as mean ± SD to compare of efficacy of MTA and calcium hydroxide on different microorganisms. Both MTA and Ca(OH)2 were found to produce zone of inhibition against Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853), , Bacillus subtilis (BTCC 17 ), and Candida albicans (BTCC 493). MTA showed highest activity against S. aureus and lowest activity against P. aeruginosa which was similar to the activity range of Ca (OH)2 against the mentioned organisms. But both of them failed to produce any activity against E. coli and. E. faecalis. MTA was found to produce a lower efficacy than Ca (OH)2 while comparing the zone of inhibition between them and statistically it was significant. Mineral Tri Oxide Aggregate (MTA) showed antimicrobial efficacy against some therapy resistant microorganisms but it did not show antimicrobial efficacy against Escherichia coli and Enterococcus faecalis. MTA was found to produce a lower antimicrobial efficacy than Ca (OH)2.J Bangladesh Coll Phys Surg 2015; 33(3): 140-145

scholarly journals AN IN VITRO COMPARISON OF ANTIMICROBIAL EFFICACY OF TRIPLE ANTIBIOTIC PASTE, CALCIUM HYDROXIDE AND ACACIA NILOTICA (BABBULA) AGAINST ENTEROCOCCUS FAECALIS, STREPTOCOCCUS MUTANS AND CANDIDA ALBICANS AS INTRACANAL MEDICAMENTS.

2021 ◽  
pp. 1-4
Author(s):  
Sumarlangki Sayoo ◽  
Shubhashini N. ◽  
Geeta I. B. ◽  
Bindu S
Keyword(s):  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Acacia Nilotica ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Triple Antibiotic Paste

Aim: The aim of this study was to compare the antimicrobial efficacy of Triple antibiotic paste (TAP), Calcium hydroxide and Acacia nilotica (A. nilotica) (Babbula) against Enterococcus faecalis (E. faecalis), Streptococcus mutans (S. mutans) and Candida albicans (C. albicans) as intracanal medicaments. Materials and methods: The triple antibiotic paste mixture of (metronidazole, ciprofloxacin and minocycline), calcium hydroxide, Acacia nilotica (Babbula) were weighed and mixed with polyethylene glycol and agar well diffusion method was employed to study the antimicrobial efficiency against Enterococcus faecalis, Streptococcus mutans and Candida albicans Results: All the intracanal medicaments showed zone of inhibition against Enterococcus faecalis, Streptococcus mutans and Candida albicans. Among all intracanal medicaments used in the study Triple antibiotic paste produced maximum zone of inhibition followed by Babbula and least by Calcium hydroxide. Conclusions: Triple antibiotic paste showed the best antimicrobial efficacy followed by Babbula and least by Calcium hydroxide against Enterococcus faecalis, Streptococcus mutans and Candida albicans. More phytochemical studies need to be done. Further evaluation of concentrated extracts of Acacia nilotica (Babbula) could yield better results.

scholarly journals Antimicrobial Efficacy of Octenidine Hydrochloride, MTAD and Chlorhexidine Gluconate Mixed with Calcium Hydroxide

2013 ◽  
Vol 14 (3) ◽  
pp. 456-460 ◽  
Author(s):  
Resmiye Ebru Tirali ◽  
Kamran Gulsahi ◽  
Sevi Burcak Cehreli ◽  
Zeynep Ceren Karahan ◽  
Emel Uzunoǧlu ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Brain Heart Infusion ◽  
Chlorhexidine Gluconate ◽  
Antimicrobial Action ◽  
Antimicrobial Efficacy ◽  
Zones Of Inhibition ◽  
Antibacterial And Antifungal

ABSTRACT Objective The aim of this in vitro study was to investigate whether mixing with calcium hydroxide [Ca(OH)2] affects the antimicrobial action of Octenidine hydrochloride (Octenisept), MTAD and chlorhexidine against Enterococcus faecalis and Candida albicans. Materials and methods Freshly grown cultures of Enterococcus faecalis, Candida albicans and a mixture of both strains were incubated in agar plates containing brain-heart infusion broth (BHIB). Zones of inhibition were measured at 24 and 48 hours. Statistical analysis was performed using Mann-Whitney U test and Kruskal-Wallis one-way analysis of variance (ANOVA, both p = 0.05). Results Mixing with Ca(OH)2 significantly increased the antibacterial effect of Octenisept (p < 0.05), but did not alter its antifungal activity. Only chlorhexidine showed more antibacterial and antifungal efficiency compared to its Ca(OH)2-mixed version (both p < 0.05). Mixing with Ca(OH)2 decreased the antibacterial efficacy of MTAD, but increased its antifungal effect (both p < 0.05). Conclusion These results demonstrate the differential effects of Ca(OH)2 addition on the antimicrobial action of the tested endodontic medicaments in vitro. Ca(OH)2 was as effective as its combination with all of the tested medicaments. How to cite this article Tirali RE, Gulsahi K, Cehreli SB, Karahan ZC, Uzunoğlu E, Elhan A. Antimicrobial Efficacy of Octenidine Hydrochloride, MTAD and Chlorhexidine Gluconate Mixed with Calcium Hydroxide. J Contemp Dent Pract 2013;14(3):456-460.

Antimicrobial photodynamic therapy in chronic osteomyelitis induced by Staphylococcus aureus: An in vitro and in vivo study

2012 ◽  
Author(s):  
João Alves dos Reis Júnior ◽  
Patrícia Nascimento de Assis ◽  
Garde^nia Matos Paraguassú ◽  
Isabele Cardoso Vieira de de Castro ◽  
Renan Ferreira Trindade ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Chronic Osteomyelitis ◽  
In Vivo Study ◽  
Antimicrobial Photodynamic Therapy

Photodynamic therapy on bacterial reduction in dental caries: in vivo study

2010 ◽  
Author(s):  
Alessandra Baptista ◽  
Renato Araujo Prates ◽  
Ilka Tiemy Kato ◽  
Marcello Magri Amaral ◽  
Anderson Zanardi de Freitas ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Dental Caries ◽  
In Vivo Study ◽  
Bacterial Reduction

In Vivo Study of Biological Effects of Photodynamic Therapy on Cervical Cancer

2005 ◽  
pp. 215
Author(s):  
V. A. Marquez-Lemus ◽  
B. M. Noguez-Juarez ◽  
L. Solano-Rodriguez ◽  
A. J. Perez-Zapata ◽  
O. P. Schneider-Ehrenberg ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Photodynamic Therapy ◽  
Biological Effects ◽  
In Vivo Study
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