scholarly journals The effect of hydroalcoholic extract of Crataegus monogyna on hyperglycemia, oxidative stress and pancreatic tissue damage in streptozotocin-induced diabetic rats

2018 ◽  
Vol 7 (4) ◽  
pp. 294-299 ◽  
Author(s):  
Saeed Valipour Chahardahcharic ◽  
Mahbubeh Setorki
Keyword(s):  
Oxidative Stress ◽  
Tissue Damage ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Hydroalcoholic Extract ◽  
Crataegus Monogyna

Crocin treatment decreased pancreatic atrophy, LOX-1 and RAGE mRNA expression of pancreas tissue in cholesterol-fed and streptozotocin-induced diabetic rats

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Mohammad Ehsan Bayatpoor ◽  
Saeed Mirzaee ◽  
Mohammad Karami Abd ◽  
Mohammad Taghi Mohammadi ◽  
Shima Shahyad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mrna Expression ◽  
Tissue Damage ◽  
Critical Role ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Control Treatment ◽  
Pcr Analysis ◽  
Control Group ◽  
Cholesterol Levels

AbstractObjectiveOxidative stress in diabetic mellitus is a consequence of oxidative stress, which plays a critical role in the pathogenesis of diabetic tissue damage. Receptors for advanced glycation end products and for oxidized low-density lipoproteins (LDL) have critical contribution in oxidative tissue damage. The present study investigated whether anti-diabetic effects of Crocin via modulation of mRNA expression of RAGE and LOX-1 receptors in diabetic rats.MethodsIn the current study, high-fat cholesterol (HFC) and streptozotocin (40 mg/kg) used to induce type II diabetes. Experimental groups as follows: (Group 1: control); (Group 2: control treatment [Crocin]); (Group 3: DM [STZ]); (Group 4: DM treatment [STZ + Crocin]); (Group 5; DM + HFC [STZ + HFC]); (Group 6; DM + HFC treatment [STZ + HFC + Crocin]). Crocin (20 mg/kg/day, i.p.) administered in treatment groups for 60 days. Serum glucose and cholesterol levels evaluated on days 5, 30 and 60 after induction of DM. Pancreatic tissue from all group removed on day 60 for histological and RT-PCR analysis.ResultsApplication of Crocin significantly decreased serum cholesterol levels on day 60 after induction of DM in diabetic + HFC rats. Moreover, Crocin significantly decreased serum glucose levels on days 30 and 60 both in diabetic and diabetic + HFC rats. Crocin partially prevented the atrophic effects of STZ on both exocrine and endocrine parts of pancreas. Additionally, Crocin significantly decreased LOX-1 and RAGE mRNA expression OF pancreas in diabetic rats.ConclusionThe current study suggested that Crocin suppressed atrophic change of the pancreas by decrease of LOX-1 and RAGE mRNA expression in diabetic rats.

scholarly journals The Effect of Dandang Gendis Extract (Clinacanthus nutans) on Kidney Histopathological Features of Diabetic Rats Model

2021 ◽  
Vol 5 (01) ◽  
pp. 19-22
Author(s):  
Miranda Jemyma Mas'ulun ◽  
Arifa Mustika ◽  
Ema Qurnianingsih
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
Tissue Damage ◽  
Diabetic Rats ◽  
Keywords Diabetes ◽  
Standard Drug ◽  
Histopathological Features ◽  
Clinacanthus Nutans ◽  
Minimize Tissue Damage ◽  
Damage Criteria

Changes in kidney homeostasis due to diabetes can cause oxidative stress which then caused tissue damage that leads to diabetic nephropathy. Clinacanthus nutans extract is known to contain antioxidants that are reported to play an important role in the body’s defense system against oxidative stress to minimize tissue damage. This study aims to know the effect of Clinacanthus nutans leaf extract administration on kidney histopathological features of the diabetic rats model. A total of 35 rats were induced by streptozotocin which then divided into 5 groups and given Clinacanthus nutans extract with a dose of 75 mg/kgBW, 150 mg/kgBW and 300 mg/kgBW then compare with CMC-Na as control and metformin as standard drug for 14 days. The kidney histopathology was evaluated under a light microscope against the damage criteria that occurred in the proximal tubules of the kidney. As the result the least amout of kidney damage was on treatment group at dose 300 mg/kgBW, followed by extract with dose 75 mg/kgBW, 150 mg/kgBW, and metformin. In conclusion Clinacanthus nutans extract with a dose of 75 mg/kgBW, 150 mg/kgBW, and 300 mg/kgBW can improve the kidney histopathological feature of the diabetic rats model. Keywords: diabetes mellitus; histopathology; diabetic nephropathy; Clinacanthus nutans

EXPLORATION OF PROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF ALSTONIA SCHOLARIS BARK IN STZ-INDUCED EARLY DIABETIC NEPHROPATHY MODEL IN RATS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 69-78
Author(s):  
D. D. Bandawane ◽  
S. B Jadhav ◽  
A. R. Juvekar ◽  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Diabetic Nephropathy ◽  
Reduced Glutathione ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Total Proteins ◽  
Hydroalcoholic Extract ◽  
Early Diabetic Nephropathy

Alstonia scholaris (fam. Apocynaceae) is an indigenous plant used traditionally for the treatment of diabetes and associated complications. However the nephroprotective potential of the plant is not scientifically evaluated. Objective of the present was to investigate renal protective activity of hydroalcoholic extract of A. scholaris bark (HEAS) in streptozotocin (STZ)-induced early diabetic nephropathy in rats and to focus on its possible mechanism of action. Experimental diabetes was induced in Wistar rats using single intraperitoneal injection of streptozotocin (65 mg/kg). Animals were divided in five groups (n=6) and treated with 150 mg/kg and 300 mg/kg HEAS for 4 weeks. At the end of study period, fasting blood glucose, blood urea nitrogen (BUN), serum creatinine, total proteins, serum albumin, serum insulin and glycosylated haemoglobin, superoxide dismutase, catalase, reduced glutathione and MDA in kidney were evaluated. Urine was analyzed for albumin, total proteins and creatinine clearance. Kidney and pancreas were subjected for histopathology. Significant decrease in fasting blood glucose, creatinine, albumin, BUN, total proteins and urinary total proteins was observed. Significant improvement in serum insulin, glycosylated Hb, oxidative stress parameters of kidney including superoxide dismutase, catalase and reduced glutathione has been observed in HEAS treated diabetic rats. Histopathology of kidney and pancreatic tissues showed structural improvement. Present study has revealed that HEAS prevented the progression of diabetic nephropathy in STZ-diabetic rats by improving the disturbed glucose homeostasis and by amelioration of renal oxidative stress.

scholarly journals Protective Effect of Hydroalcoholic Extract of Solanum surattense on Brain Tissue Damage and Oxidative Stress in Adult Rats with Toxoplasmosis

2020 ◽  
Vol 1 (1) ◽  
pp. 14-17
Author(s):  
Mansour Ataei ◽  
Arash Khaki ◽  
Yagoob Garedaghi
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Brain Tissue ◽  
Tissue Damage ◽  
Female Rats ◽  
Adult Rats ◽  
Hydroalcoholic Extract ◽  
Solanum Surattense ◽  
Brain Tissue Damage ◽  
The Brain

Introduction: Toxoplasmosis is caused by a protozoan named Toxoplasma gondii. This protozoan is a parasite of cats that can spread among other animals and birds around the world and cause a disease that varies from mild to severe. The disease is seen in the forms of acquired toxoplasmosis and congenital toxoplasmosis. Many studies have shown that there is a relationship between reproductive function and toxoplasmosis. T. gondii has led to decreased reproductive performance of males and females in many experimental animals. The aim of this study was to investigate the protective effect of hydroalcoholic extract of Solanum surattense on the brain tissue damage and brain oxidative stress induced by T. gondii in adult rats. Methods: For this purpose, 32 adult female rats were randomly divided into 4 groups. In group 1, 8 healthy rats received IP saline for 3 weeks. In group 2, 8 rats with T. gondii received IP saline for 3 weeks. In group 3, 8 rats with T. gondii received the hydroalcoholic extract of S. surattense for 3 weeks. In group 4, 8 healthy rats received the hydroalcoholic extract of S. surattense for 3 weeks. Then, brain tissue resection was performed to evaluate histological damage and levels of antioxidant enzymes. Results: Histological and biochemical studies showed that T. gondii had a deleterious effect on the brain tissue of rats and increased the level of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). The administration of hydroalcoholic extract of S. surattense improved these effects due to its high antioxidant properties. Conclusion: The administration of the appropriate dose of hydroalcoholic extract of S. surattense for three consecutive weeks had a protective effect on brain tissue exposed to T. gondii.

scholarly journals Metformin on Insulin, Glucagon, Oxidative Stress Markers and Pancreatic Tissue Histology in Streptozotocin-Induced Diabetic Rats

2021 ◽  
Vol 6 (3) ◽  
pp. 24-29
Author(s):  
Umar Zayyanu Usman ◽  
Abdullahi Suleiman Mainasara ◽  
Abdullahi Yahaya Abbas ◽  
Mas UD Eneji Sadiq ◽  
Mahaneem Mohamed
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Body Weight ◽  
Protein Carbonyl ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Diabetes Mellitus Group ◽  
Metformin Group ◽  
Total Antioxidant ◽  
Tissue Histology

This study determined the effects of Metformin on plasma insulin, glucagon, oxidative stress status and histology of pancreas in diabetic rats. Grouping was carried out on 21 rats, and were assigned into 3 groups (n = 7 rats per group); non-DM, DM and DM+metformin (200 mg/kg). Diabetes Mellitus was induced and treatments were given daily by oral gavage for 4 weeks. Food intake, fasting blood glucose (FBG), glucagon, malondialdehyde and protein carbonyl were significantly higher while final body weight, insulin and total antioxidant capacity were significantly lower in Diabetes Mellitus group compared with non- Diabetes Mellitus group. There was significantly higher body weight, insulin level and total antioxidant capacity with significantly lower food intake, FBG, glucagon, malondialdehyde and protein carbonyl levels in Diabetes Mellitus +Metformin group compared with the Diabetes Mellitus group. Pancreatic tissue histology revealed islet cells regeneration in Diabetes Mellitus +Metformin group. This in vivo study provides evidence of the potential of antihyperglycemic and improved oxidants- antioxidant status which may be due to the oral hypoglycaemic nature or effects of metformin as seen in this study.

scholarly journals Effect of Katakakhadiradi Kashayam on Lipid Profile and Pancreatic Damage in Type II Diabetes Mellitus

2021 ◽  
pp. 135-141
Author(s):  
Angelie Jessica Subbiah ◽  
M. Kavimani ◽  
Mukilan Ramadoss ◽  
Mudiganti Ram Krishna Rao ◽  
K. Prabhu
Keyword(s):  
Lipid Profile ◽  
Tissue Damage ◽  
Serum Lipid ◽  
Total Cholesterol Level ◽  
Hdl Cholesterol ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Serum Lipid Profile ◽  
Standard Drug ◽  
Pancreatic Damage

The lipid pattern and levels in diabetic patient are the same as those for subjects with cardiovascular disease. The tactic underlying the oral hypoglycemic agent is to adjust the lipid profile; which can be achieved by herbal therapy. The use of herbs and formulations for attenuation of hyperglycemia and to aid protection against the pancreatic damage is clinically very important. This study was intended to find the efficacy of Katakakhadiradi Kashayam(KKK)on lipid profile and pancreatic tissue damage in streptozotocin-nicotinamide (SN) induced diabetic rats. The diabetic rats were treated with Katakakhadiradi Kashyam orally at doses of 100, 200 and 300 mg/kg/bw. For 28 days and compared with the standard drug Glibenclamide. After the kashayam treatment triglyceride, HDL-cholesterol and total cholesterol level were assayed and pancreatic tissue damage caused by streptozotocin was analysed by histology study.Katakakhadiradi kashayam could restore the serum lipid profile by controlling the blood glucose level and reduce the pancreatic injury in diabetic rats. Supplementation of Katakakhadiradi kashayam showed a significant improvement in the serum lipid profile thus helping in retarding the secondary complications.

Protective role of aqueous extract of Phyllanthus amarus on oxidative stress in pancreas of streptozotocin induced diabetic male Wistar rats

2016 ◽  
Vol 2 (1) ◽  
pp. 23 ◽  
Author(s):  
Sasi Bhusana Rao Bongu ◽  
Saisree Sagree ◽  
Vijayabharathi Gudapareddy ◽  
Malliah Putakala ◽  
Sreenivasulu Nukala ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Reduced Glutathione ◽  
Protective Role ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Phyllanthus Amarus ◽  
Glutathione S Transferase ◽  
Male Wistar Rats

Diabetes mellitus has been associated with oxidative stress. The present study was performed to investigate the pancreas protective activities of Phyllanthus amarus aqueous extract (PAAEt) in streptozotocin (STZ) induced diabetic rats. Oral administration of PAAEt (200mg/kg bw) to diabetic rats for 60 days significantly (p<0.05) decreased antioxidant enzymes such as glutathione peroxidase, glutathione-s-transferase, glutathione reductase, superoxide dismutase, catalase and depletion of reduced glutathione in panceas which illustrates that increased lipid peroxidation (107 %) was restored to control levels. Co-treatment with PAAEt revealed the therapeutic efficacy against oxidative stress in pancreatic tissue. Hence extract of this plant could be used as an adjuvant therapy for the prevention and/or management of diabetes.

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