scholarly journals Genotype Characterization of Human Hydatid Cyst Isolates From Patients in Karaj, Iran, Using COX1 Gene Sequence

2019 ◽  
Vol 7 (1) ◽  
pp. 27-29
Author(s):  
Maryam Salimi ◽  
Abolfazl Miahipour ◽  
Mohammad Zibaei ◽  
Sasan Rezaie
Keyword(s):  
Hydatid Cyst ◽  
Gene Sequence ◽  
Cox1 Gene ◽  
Zoonotic Infection ◽  
Pcr Assay ◽  
Genetic Characteristics ◽  
Control And Prevention ◽  
Polymerase Chain ◽  
Clinical Problems

Background: Cystic echinococcosis is a main zoonotic infection. It can cause serious clinical problems for human health around the world. Genotypic specification of Echinococcus granulosus in human is important due to control and prevention programs. Objective: In this investigation, genetic characteristics of human isolates of E. granulosus in Karaj, Iran, were studied. Materials and Methods: In this review, 3 isolates of surgically removed hydatid cysts were obtained from patients in Shahid Madani hospital, Karaj, Iran in 2014. DNA was extracted from the protoscolex of the cyst, and polymerase chain reaction (PCR) assay was done on the COX1 gene. Results: DNA fragments were sequenced and the results were aligned and analyzed. Among the isolates, 3 (100%) were E. granulosus (G1) strain. Conclusion: The G1 genotype was the most superior strain from human isolates of hydatid cyst in Karaj.

scholarly journals Genotyping of Fresh and Parafinized Human Hydatid Cysts Using nad1 and cox1 Genes in Hamadan Province, West of Iran

2020 ◽  
Author(s):  
Ali Ehsan SHAHBAZI ◽  
Massoud SAIDIJAM ◽  
Amir Hossein MAGHSOOD ◽  
Mohammad MATINI ◽  
Moosa MOTAVALI HAGHI ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Cox1 Gene ◽  
Hydatid Cysts ◽  
Zoonotic Infection ◽  
Pcr Assay ◽  
The West ◽  
Sequence Variations ◽  
Sequences Analysis ◽  
Genotype G1

Background: Hydatidosis is a cosmopolitan zoonotic infection and Hamadan Province in the west of Iran is one of the most important foci of human hydatidosis in Iran. The aim of the current study was the genetic characterization of hydatid cysts operated from humans in Hamadan Province. atid cysts operated from humans in Hamadan Province. Methods: Seventy-two hydatid cysts samples including 50 paraffinized and 22 fresh human hydatid cysts collected from different hospitals in Hamadan Province, western Iran. The cysts' DNA genome was extracted by kit and PCR was performed for amplifying the fragments of 400 and 450bp for nad1 and cox1 mitochondrial genes, respectively. Genotype diversity and sequence variations of the cysts' isolates were studied by related software. Results: DNA from all (100%) paraffinized and fresh hydatid cysts samples extracted successfully. All paraffinized and fresh hydatid cysts samples were amplified by PCR assay using nad1gene, however, only 18 and 8 samples from paraffinized and fresh hydatid cyst samples was amplified using cox1 gene, respectively. The sequences analysis indicated that, 98.61% the Echinococcus granulosus samples were belong to the genotype G1 and 1.39% were G3 genotype. Conclusion: Genotypes of E. granulosus in human samples in Hamadan Province are G1 and G3 and these findings are proved by phylogenic analysis.  

scholarly journals Descriptive Investigation of Strongyloidiasis Infection and Characterization of Strongyloides stercoralis Using Morphological and Molecular-Based Methods

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Nayana Gunathilaka ◽  
Nilmini Chandrasena ◽  
Tharaka Wijerathna ◽  
Yoshito Fuji ◽  
Deepa Gunasekara ◽  
...  
Keyword(s):  
Gastrointestinal Symptoms ◽  
Strongyloides Stercoralis ◽  
Molecular Evidence ◽  
Pcr Assay ◽  
Its1 Region ◽  
Promising Tool ◽  
Hyperinfection Syndrome ◽  
Polymerase Chain ◽  
Stool Cultures

Strongyloidiasis is caused by the nematode Strongyloides stercoralis which has the unique ability to reproduce and complete its entire life cycle within the human host through its autoinfection cycle. Diagnosis of this infection is important because of its potential to cause fatal hyperinfection syndrome or disseminated infections in those with defective cellular immunity. Parasitological methods based on faecal microscopy and culture often fail to detect low-intensity infections. A multiplex polymerase chain reaction (PCR) assay was developed for the detection of S. stercoralis, Ascaris lumbricoides, and Enterobius vermicularis by designing primers specific for the ITS1 region of ribosomal DNA of S. stercoralis and A. lumbricoides and 18S region of rRNA of E. vermicularis. A 61-year-old patient presented with chronic gastrointestinal and respiratory symptoms and weight loss with a stool microscopy positive for helminth larvae. Stool cultures with the Harada–Mori technique yielded L3 larvae which were identified as S. stercoralis based on morphology. The multiplex PCR performed on DNA extracted from stool elicited the expected band at 129 bp on gel electrophoresis of the PCR yield providing molecular evidence of intestinal strongyloidiasis. The patient’s gastrointestinal symptoms improved with a six-day course of albendazole (400 mg twice daily). Negative posttreatment stool microscopy, culture, and PCR confirmed successful clearance of infection. Molecular-based PCR assay is a promising tool to diagnose and assess the therapeutic efficacy of anthelmintics in intestinal helminthiases such as strongyloidiasis.

scholarly journals Prevalence and characteristics ofEscherichia coilserotype O157:H7 and other verotoxin-producingE. coliin healthy cattle

1996 ◽  
Vol 117 (2) ◽  
pp. 251-257 ◽  
Author(s):  
M. Blanco ◽  
J. E. Blanco ◽  
J. Blanco ◽  
E. A. Gonzalez ◽  
A. Mora ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Gene Sequence ◽  
Heterogeneous Population ◽  
Chain Reaction ◽  
E Coli ◽  
Healthy Cattle ◽  
Faecal Samples ◽  
Polymerase Chain

SummaryFrom February to July of 1994, 328 faecal samples from 32 herds were collected and verotoxin-producingEscherichia coli(VTEC) found on 84% of the farms. The proportion of animals infected varied from 0–63%. VTEC were recovered from 52 (20%) of 257 cows and from 16 (23%) of 71 calves. Although the VTEC belonged to 25 different serogroups, 7 (O8. O20, O22, O77, O113, O126 and O162) accounted for 46% of strains. Nearly 45% of the 83 bovine VTEC strains belonged to serogroups associated with haemorrhagic colitis and haemolytic uraernic syndrome in humans. However, only 2 (2%) of 83 VTEC strains isolated from cattle belonged to enterohaemorrhagicE. coli(EHEC) serotypes (O26:H11 and O157:H7), and only 8 (10%) were positive for the attaching and effacingE. coli (eae)gene sequence. Polymerase chain reaction (PCR) showed that 17 (20%) of VTEC strains carried VT1 genes. 43 (52%) possessed VT2 genes, and 23 (28%) carried both VT1 and VT2 genes. Characterization of VTEC isolates revelated a heterogeneous population in terms of serogroup and toxin type in the positive herds. This study confirms that healthy cattle are a reservoir of VTEC, but, the absence ofeaegenes in most bovine VTEC strains suggests that they may be less virulent for humans thaneae-positive EHEC.

scholarly journals Detection of Maize Intrinsic and Recombinant Cry1Ab Gene Fragment in Genetically Modified Maize

1970 ◽  
Vol 17 (1) ◽  
pp. 103-108
Author(s):  
T Rahman ◽  
EH Chowdhury ◽  
AC Mondol ◽  
MM Hoque ◽  
KM Nasiruddin
Keyword(s):  
Gene Sequence ◽  
Genetically Modified ◽  
Gene Fragment ◽  
Genetically Modified Maize ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Cry1ab Gene ◽  
Intrinsic Gene ◽  
Bt Gene ◽  
Polymerase Chain

Polymerase chain reaction (PCR) assay was performed to detect and identify the genetically modified maize (Bt11). Primer pair Bt11 1-5´ and Cry1Ab 1-3´ detected the region of the insect resistant Cry1Ab gene sequence inserted in GM Bt11 maize and a primer pair ZE01-ZE02 were used to detect the maize intrinsic zein (Ze1) gene in maize DNA. The presence of the corresponding DNA segments was specifically detected in GM maize by the designed primers. It was concluded that this method is useful for fast and easy screening of Bt gene in the food products and GM Bt crops.Key words: Detection, Cry1Ab gene, Maize intrinsic gene, GM maizeDOI = 10.3329/ptcb.v17i1.1127Plant Tissue Cult. & Biotech. 17(1): 103-108, 2007 (June)

scholarly journals Molecular characterization of Eurytrema coelomaticum in cattle from Paraná, Brazil

2014 ◽  
Vol 23 (3) ◽  
pp. 383-386 ◽  
Author(s):  
Gustavo Freire Figueira ◽  
Victor Henrique Silva de Oliveira ◽  
Alessandra Taroda ◽  
Amauri Alcindo Alfieri ◽  
Selwyn Arlington Headley
Keyword(s):  
Molecular Characterization ◽  
Gene Sequence ◽  
Domestic Animals ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Analyses ◽  
Pcr Assay ◽  
Bovine Pancreas

This study investigated the occurrence of Eurytremaspp. in cattle by analysis of the partial 18S rRNA gene sequence. Trematodes from 44 bovine pancreas were collected and classified based on typical morphological features. PCR assay and sequence analyses of amplified products confirmed that the trematodes classified as Eurytrema coelomaticum were phylogenetically distinct from those identified as E. pancreaticum. The results of this study represent the first molecular characterization of E. coelomaticum within the Americas, and provide an efficient method to differentiate digenean trematodes of domestic animals.

scholarly journals Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002

1970 ◽  
Vol 3 (2) ◽  
pp. 36-39
Author(s):  
Muhammad Suhaib Mat Hussin ◽  
Mohd Hasnain Hussain ◽  
Awang Ahmad Sallehin Awang Husaini ◽  
Koplo Bujang ◽  
Dayang Salwani Awg Adeni ◽  
...  
Keyword(s):  
Bacillus Amyloliquefaciens ◽  
Gene Sequence ◽  
In Silico Analysis ◽  
Chain Reaction ◽  
Gene Encoding ◽  
Gene Characterization ◽  
Polymerase Chain ◽  
Sago Pith Waste ◽  
Silico Analysis

Characterization of α-amylase gene sequence produced by Bacillus amyloliquefaciens UMAS 1002, acellulolytic and amylolytic bacilli isolated from sago pith waste is described here. The amyE gene encoding theα-amylase was isolated by polymerase chain reaction. The 1,980 bp of amyE gene corresponding to 660 aminoacids showed 99% homology to the α-amylase sequence from Bacillus subtilis X-23 (GenBank: BAA31528).The α-amylase sequence of B. amyloliquefaciens UMAS 1002 (GenBank: KC800929) differs from that of B.subtilis X-23 by 5 amino acids. In silico analysis of α-amylase from B. amyloliquefaciens UMAS 1002 showedsimilar characteristics compared to α-amylase from B. subtilis X-23.

scholarly journals Characterization of Xanthomonas hortorum pv. pelargonii Isolated from Geranium in Serbia

Plant Disease ◽  
2016 ◽  
Vol 100 (1) ◽  
pp. 164-170
Author(s):  
Jelica Balaž ◽  
Žarko Ivanović ◽  
Andrej Davidović ◽  
Renata Iličić ◽  
Jaap Janse ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Blight ◽  
Gene Sequence ◽  
Chain Reaction ◽  
Pelargonium Zonale ◽  
Molecular Assays ◽  
Pathogenicity Tests ◽  
Polymerase Chain ◽  
Phenotypic Tests

Geranium leaves and stems with symptoms of bacterial blight were collected from commercial greenhouses during the last decade in Serbia. In total, 17 isolates with colony morphology typical for the genus Xanthomonas were characterized with pathogenicity, biochemical, serological, and molecular assays. All 17 isolates reacted positive in a polymerase chain reaction (PCR) using XcpM1 and XcpM2 primers specific for Xanthomonas hortorum pv. pelargonii. In pathogenicity tests on Pelargonium zonale (leaf and stem inoculation), all isolates caused typical symptoms on leaves starting 2 days after inoculation as sunken, water-soaked, irregular lesions, and 6 to 8 days after inoculation on stems as necrotic lesions also showing yellow exudate. Symptoms resulted in general wilting of inoculated plants 20 days after inoculation. Selected phenotypic tests indicated that all isolates showed the same results as described for the bacterium X. hortorum pv. pelargonii. Repetitive sequence-based PCR typing using BOX and ERIC revealed that all isolates showed two fingerprinting profiles but (GTG)5 and REP did not reveal differences. Multilocus sequence typing of partial sequences of rpoD, dnaK, fyuA, and gyrB genes of tested isolates and sequences obtained from GenBank of Xanthomonas pathovar pathotype strains did not reveal genetic variability among the isolates, showing the same gene sequence pattern.

scholarly journals Detection and Characterization of Porcine Sapelovirus in Italian Pig Farms

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 966
Author(s):  
Eleonora Chelli ◽  
Luca De Sabato ◽  
Gabriele Vaccari ◽  
Fabio Ostanello ◽  
Ilaria Di Bartolo
Keyword(s):  
Phylogenetic Analyses ◽  
Whole Genome Sequence ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Genetic Characteristics ◽  
The Family ◽  
Polymerase Chain ◽  
Porcine Sapelovirus

Porcine sapelovirus (PSV) belongs to the genus Sapelovirus of the family Picornaviridae. PSV infects pigs asymptomatically, but it can also cause severe neurologic, enteric, and respiratory symptoms or reproductive failure. Sapelovirus infections have been reported worldwide in pigs. The objective of this study was to investigate the presence and the prevalence of PSV in Italian swine farms in animals of different ages to clarify the occurrence of the infection and the genetic characteristics of circulating strains. In the present study, 92 pools of fecal samples, collected from pigs across three farms, were analyzed by Reverse Transcriptase-polymerase Chain Reaction-PCR (RT-PCR). Fecal pools from young growers (63/64) were found positive for Sapelovirus in all farms while detection in sows (4/28) was observed in only one farm. Phylogenetic analyses of the 19 partial capsid protein nucleotide sequences (VP1) (6–7 each farm) enable the classification of the virus sequences into three distinct clades and highlighted the high heterogeneity within one farm. The whole genome sequence obtained from one strain showed the highest correlation with the Italian strain detected in 2015. The study adds novel information about the circulation and heterogeneity of PSV strains in Italy and considering the movement of pigs across Europe would also be informative for other countries.

scholarly journals Genotypic characterization of virulence factors in Escherichia coli strains from patients with cystitis

2008 ◽  
Vol 50 (5) ◽  
pp. 255-260 ◽  
Author(s):  
Monique Ribeiro Tiba ◽  
Tomomasa Yano ◽  
Domingos da Silva Leite
Keyword(s):  
Escherichia Coli ◽  
Iron Acquisition ◽  
Uropathogenic Escherichia Coli ◽  
Pcr Assay ◽  
Single Strain ◽  
Polymerase Chain ◽  
Factor Type ◽  
Tract Infections

Adhesins (P-fimbriae, S-fimbriae, type 1 fimbriae and afimbrial adhesin), toxins (α-hemolysin and cytotoxic necrotizing factor type 1), iron acquisition systems (aerobactin) and host defense avoidance mechanisms (capsule or lipopolysaccharide) have been shown to be prevalent in Escherichia coli strains associated with urinary tract infections. In this work, 162 Uropathogenic Escherichia coli (UPEC) strains from patients with cystitis were genotypically characterized by polymerase chain reaction (PCR) assay. We developed three multiplex PCR assays for virulence-related genes papC, papE/F, papG alleles, fimH, sfa/foc, afaE, hly, cnf-1, usp, cdtB, iucD, and kpsMTII, all of them previously identified in UPEC strains. The PCR assay results identified 158 fimH (97.5%), 86 kpsMTII (53.1%), 53 papC/papEF/papG (32.7%), 45 sfa (27.8%), 42 iucD (25.9%), 41 hly (25.3%), 36 usp (22.2%), 30 cnf-1(18.5%) and 10 afa (6.2%) strains. No strain was positive for cdtB. In this work, we also demonstrated that adhesins may be multiple within a single strain and that several virulence genes can occur combined in association.

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