scholarly journals Tetracycline Resistance Genes in Salmonella enterica Serovars With Animal and Human Origin

2018 ◽  
Vol 6 (3) ◽  
pp. 60-64
Author(s):  
Rahem Khoshbakht ◽  
Abdollah Derakhshandeh ◽  
Leila Jelviz ◽  
Fatemeh Azhdari
Keyword(s):  
Resistance Genes ◽  
Salmonella Enterica ◽  
Tetracycline Resistance ◽  
Human Origin ◽  
Tetracycline Resistance Genes

scholarly journals Novel Insertion Sequence- and Transposon-Mediated Genetic Rearrangements in Genomic Island SGI1 of Salmonella enterica Serovar Kentucky

2008 ◽  
Vol 52 (10) ◽  
pp. 3745-3754 ◽  
Author(s):  
Benoît Doublet ◽  
Karine Praud ◽  
Sophie Bertrand ◽  
Jean-Marc Collard ◽  
François-Xavier Weill ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Salmonella Enterica ◽  
Genomic Island ◽  
Tetracycline Resistance ◽  
Gene Clusters ◽  
Mercury Resistance ◽  
Reading Frame ◽  
Tetracycline Resistance Genes ◽  
Serovar Typhimurium ◽  
Genetic Rearrangements

ABSTRACT Salmonella genomic island 1 (SGI1) is an integrative mobilizable element that harbors a multidrug resistance (MDR) gene cluster. Since its identification in epidemic Salmonella enterica serovar Typhimurium DT104 strains, variant SGI1 MDR gene clusters conferring different MDR phenotypes have been identified in several S. enterica serovars and classified as SGI1-A to -O. A study was undertaken to characterize SGI1 from serovar Kentucky strains isolated from travelers returning from Africa. Several strains tested were found to contain the partially characterized variant SGI1-K, recently described in a serovar Kentucky strain isolated in Australia. This variant contained only one cassette array, aac(3)-Id-aadA7, and an adjacent mercury resistance module. Here, the uncharacterized part of SGI1-K was sequenced. Downstream of the mer module similar to that found in Tn21, a mosaic genetic structure was found, comprising (i) part of Tn1721 containing the tetracycline resistance genes tetR and tet(A); (ii) part of Tn5393 containing the streptomycin resistance genes strAB, IS1133, and a truncated tnpR gene; and (iii) a Tn3-like region containing the tnpR gene and the β-lactamase bla TEM-1 gene flanked by two IS26 elements in opposite orientations. The rightmost IS26 element was shown to be inserted into the S044 open reading frame of the SGI1 backbone. This variant MDR region was named SGI1-K1 according to the previously described variant SGI1-K. Other SGI1-K MDR regions due to different IS26 locations, inversion, and partial deletions were characterized and named SGI1-K2 to -K5. Two new SGI1 variants named SGI1-P1 and -P2 contained only the Tn3-like region comprising the β-lactamase bla TEM-1 gene flanked by the two IS26 elements inserted into the SGI1 backbone. Three other new variants harbored only one IS26 element inserted in place of the MDR region of SGI1 and were named SGI1-Q1 to -Q3. Thus, in serovar Kentucky, the SGI1 MDR region undergoes recombinational and insertional events of transposon and insertion sequences, resulting in a higher diversity of MDR gene clusters than previously reported and consequently a higher diversity of MDR phenotypes.

scholarly journals Antimicrobial Use and Resistance in Swine Waste Treatment Systems

2006 ◽  
Vol 72 (12) ◽  
pp. 7813-7820 ◽  
Author(s):  
Archana Jindal ◽  
Svetlana Kocherginskaya ◽  
Asma Mehboob ◽  
Matthew Robert ◽  
Roderick I. Mackie ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Waste Treatment ◽  
Growth Promotion ◽  
Tetracycline Resistance ◽  
Land Application ◽  
Molecular Methods ◽  
23S Rrna ◽  
Organic Farm ◽  
Tetracycline Resistance Genes ◽  
Fecal Streptococci

ABSTRACT Chlortetracycline and the macrolide tylosin were identified as commonly used antimicrobials for growth promotion and prophylaxis in swine production. Resistance to these antimicrobials was measured throughout the waste treatment processes at five swine farms by culture-based and molecular methods. Conventional farm samples had the highest levels of resistance with both culture-based and molecular methods and had similar levels of resistance despite differences in antimicrobial usage. The levels of resistance in organic farm samples, where no antimicrobials were used, were very low by a culture-based method targeting fecal streptococci. However, when the same samples were analyzed with a molecular method detecting methylation of a specific nucleotide in the 23S rRNA that results in resistance to macrolides, lincosamides, and streptogramin B (MLSB), an unexpectedly high level of resistant rRNA (approximately 50%) was observed, suggesting that the fecal streptococci were not an appropriate target group to evaluate resistance in the overall microbial community and that background levels of MLSB resistance may be substantial. All of the feed samples tested, including those from the organic farm, contained tetracycline resistance genes. Generally, the same tetracycline resistance genes and frequency of detection were found in the manure and lagoon samples for each commercial farm. The levels of tetracycline and MLSB resistance remained high throughout the waste treatment systems, suggesting that the potential impact of land application of treated wastes and waste treatment by-products on environmental levels of resistance should be investigated further.

Abundances of Tetracycline Resistance Genes and Tetracycline Antibiotics during Anaerobic Digestion of Swine Waste

2019 ◽  
Vol 48 (1) ◽  
pp. 171-178 ◽  
Author(s):  
Melanie Couch ◽  
Getahun E. Agga ◽  
John Kasumba ◽  
Rohan R. Parekh ◽  
John H. Loughrin ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Resistance Genes ◽  
Tetracycline Resistance ◽  
Swine Waste ◽  
Tetracycline Antibiotics ◽  
Tetracycline Resistance Genes

scholarly journals Tetracycline Susceptibility Testing and Resistance Genes in Isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus Complex from a U.S. Military Hospital

2009 ◽  
Vol 53 (6) ◽  
pp. 2693-2695 ◽  
Author(s):  
Kevin S. Akers ◽  
Katrin Mende ◽  
Heather C. Yun ◽  
Duane R. Hospenthal ◽  
Miriam L. Beckius ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Susceptibility Testing ◽  
Tetracycline Resistance ◽  
Acinetobacter Calcoaceticus ◽  
Multidrug Resistant ◽  
Military Hospital ◽  
Tetracycline Resistance Genes ◽  
Testing Method

ABSTRACT Infections with multidrug-resistant Acinetobacter baumannii-Acinetobacter calcoaceticus complex bacteria complicate the care of U.S. military personnel and civilians worldwide. One hundred thirty-three isolates from 89 patients at our facility during 2006 and 2007 were tested by disk diffusion, Etest, and broth microdilution for susceptibility to tetracycline, doxycycline, minocycline, and tigecycline. Minocycline was the most active in vitro, with 90% of the isolates tested susceptible. Susceptibilities varied significantly with the testing method. The acquired tetracycline resistance genes tetA, tetB, and tetA(39) were present in the isolates.

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