scholarly journals Human Bone Marrow-Derived Mesenchymal Cell Reactions to 316L Stainless Steel: An in Vitro Study on Cell Viability and Interleukin-6 Expression

2017 ◽  
Vol 7 (2) ◽  
pp. 335-338 ◽  
Author(s):  
Iwan Budiwan Anwar ◽  
Asep Santoso ◽  
Eko Saputra ◽  
Rifky Ismail ◽  
J. Jamari ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stainless Steel ◽  
Cell Viability ◽  
Interleukin 6 ◽  
316L Stainless Steel ◽  
In Vitro Study ◽  
Mesenchymal Cell ◽  
Human Bone ◽  
Human Bone Marrow

In vitro study of the proliferation and growth of human bone marrow cells on apatite–wollastonite-2M glass ceramics

2010 ◽  
Vol 6 (6) ◽  
pp. 2254-2263 ◽  
Author(s):  
M. Magallanes-Perdomo ◽  
A.H. De Aza ◽  
A.Y. Mateus ◽  
S. Teixeira ◽  
F.J. Monteiro ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Glass Ceramics ◽  
In Vitro Study ◽  
Human Bone ◽  
Vitro Study ◽  
Human Bone Marrow ◽  
Marrow Cells

scholarly journals An in vitro Evaluation of the Cytotoxicity of Varying Concentrations of Sodium Hypochlorite on Human Mesenchymal Stem Cells

2014 ◽  
Vol 15 (4) ◽  
pp. 473-481 ◽  
Author(s):  
Zeeshan H Ahmad ◽  
Sarah M Alkahtany ◽  
Sukumaran Anil
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Sodium Hypochlorite ◽  
Human Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

ABSTRACT Aim To evaluate and compare the cytotoxicity of various concentrations of sodium hypochlorite on immortalized human bone marrow mesenchymal stem cells (MSCs). Materials and methods The 5.25 percent sodium hypochlorite (NaOCl) at concentrations of 0.5, 0.1, 0.025, 0.0125, and 0.005 mg/ml were used to assess the cytotoxic effect on MSCs. Immortalized human bone marrow mesenchymal stem cells (hTERT-MSCs) were exposed to NaOCl at 5 different concentrations. Cell viability was assessed by 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) and alamarBlue assays. The cell morphology changes were assessed with scanning electron microscopy (SEM) after exposure to 2, 4, and 24 hour incubation. The ethidium bromide/acridine orange (EB/ AO) fluorescent stain was applied to the cells in the 8-chamber slides after they were incubated with the testing agents for 2 and 4 hours to detect live and dead cells. The observations were quantitatively and qualitatively analyzed. Results The cell viability study using MTT assay and AB assay showed significant reduction with varying concentration at 2 and 4 hours incubation period. The cell viability decreased with the higher percentage of NaOCl. The exposure time also revealed an inverse relation to the cell viability. The SEM analysis showed reduction in the number of cells and morphological alterations with 0.5 mg/ml at 2 and 4 hours compared to 0.025 mg/ml NaOCl. Destruction of the cells with structural alterations and lysis was evident under fluorescence microscope when the cells were exposed to 0.5 mg/ml NaOCl. Conclusion Within the limitations of this in vitro study it can be concluded that NaOCl is toxic to the human bone marrow MSCs. The cell lysis was evident with higher concentration of sodium hypochlorite. From the observations, it can be concluded that a lower concentration of NaOCl may be used as endodontic irrigant due to its cytotoxic properties. Further studies are man datory to evolve a consensus on the optimal concentration of sodium hypochlorite to be used as endodontic irrigant. How to cite this article Alkahtani A, Alkahtany SM, Anil S. An in vitro Evaluation of the Cytotoxicity of Varying Concentrations of Sodium Hypochlorite on Human Mesenchymal Stem Cells. J Contemp Dent Pract 2014;15(4):473-481.

scholarly journals In vitro study for stimulation effect of human bone marrow mesenchymal stem cell secretome on humen granulosa cell

2019 ◽  
Vol 112 (3) ◽  
pp. e420
Author(s):  
Hang-Soo Park ◽  
Abdeljabar El Andaloussi ◽  
Rishi Man Chugh ◽  
Amro Elsharoud ◽  
Mara Ulin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Granulosa Cell ◽  
In Vitro Study ◽  
Human Bone ◽  
Vitro Study ◽  
Human Bone Marrow

scholarly journals Effects of Micronized Cartilage Matrix on Cartilage Repair in Osteochondral Lesions of the Talus

2017 ◽  
Vol 2 (3) ◽  
pp. 2473011417S0000
Author(s):  
Christopher Kreulen ◽  
Eric Giza ◽  
Alvin Shieh ◽  
Sohni Singh ◽  
Connor Nathe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Viability ◽  
Chondrogenic Differentiation ◽  
Cartilage Matrix ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteochondral Lesions ◽  
Dead Cell

Category: Ankle, Arthroscopy, Sports Introduction/Purpose: A promising new technique in the treatment of osteochondral lesions of the talus (OLT) involves the use of an acellular micronized cartilage matrix (MCM), BioCartilage, to fill the lesions. The micronized cartilage matrix is thought to improve the production of hyaline-like cartilage by resident cells in a cartilage defect, but its effect on bone marrow cells remains untested. Here we hypothesized that adding bone-marrow derived stem cells to the BioCartilage would result in the chondrogenic differentiation of the stem cells. We designed an in-vitro model to mimic the clinical situation to determine if the combination of MCM and human bone marrow derived mesenchymal stem cells (MSCs) would produce a hyaline-like cartilage in- vitro to ultimately provide a reliable, one-step treatment for osteochondral lesions in the talus. Methods: Human bone marrow-derived stem cells were obtained from consented patients and expanded in monolayer culture using standard protocols, to a maximum passage of 4. Viability was measured using Live/Dead cell viability assays (Thermofisher), and imaged on a Nikon TE2000 inverted fluorescent microscope. A custom-manufactured polysulfone device was created with four 6 mm diameter 3 mm deep indentations in agarose within each well of standard 6-well culture plates (Figure 1A-C). In each well, we placed chrondrogenic media with cells+micronized matrix to a depth of 2 mm and covered with a 1 mm layer of TISSEEL fibrin glue as is done clinically. Control groups had either no cells, or no MCM. At the end of 3 weeks, cartilage constructs were extracted and divided to perform viability, histology, and gene expression analysis (Figure 1D). Experiments were performed with 4 technical replicates, and repeated at least 3 times. Statistical analysis was performed using ANOVA with Dunnett’s test. Results: We found that stem cells were almost immediately killed when added directly to the dry micronized cartilage powder. Rehydrating the micronized cartilage prior to addition of cells was required to maintain the viability of the added stem cells, with no statistically significant difference between rehydration with serum or saline. After 3 weeks of culture in chondrogenic media, we observed that the combination of stem cells and micronized cartilage produced a cohesive structures that were easily handled, suggesting chondrogenic differentiation of the stem cells. Without the micronized matrix, the stem cells did not form viable constructs. In constructs that contained both cells and micronized cartilage, the 3-week cell viability was over 98%, with no dead cells visible in many constructs. Conclusion: Our study demonstrates that the micronized cartilage matrix is a suitable scaffold for the chondrogenic differentiation of bone marrow-derived stem cells, given that the matrix is first rehydrated before adding cells. Technical observations include that the MCM itself generated a “dead cell” signal initially, therefore the normalized total number of live cells in each condition was used for statistical comparisons. After 3 weeks of culturing under chondrogenic media conditions, we observed robust cell survival with nearly 100% viability. Preliminary results suggest cartilage matrix deposition occurred surrounding the cells after 3 weeks of chondrogenic culture.

Interleukin-6 is a cofactor for the growth of myeloid cells from human bone marrow aspirates but does not affect the clonogenicity of myeloma cells in vitro

1990 ◽  
Vol 76 (4) ◽  
pp. 476-483 ◽  
Author(s):  
A. Montes Borinaga ◽  
B. C. Millar ◽  
J. B. G. Bell ◽  
J. K. Joffe ◽  
J. L. Millar ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Interleukin 6 ◽  
Myeloid Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Myeloma Cells
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