scholarly journals A study on the effect of parameters on lactic acid production from whey

2016 ◽  
Vol 18 (1) ◽  
pp. 58-63 ◽  
Author(s):  
Hamidreza Ghafouri Taleghani ◽  
Ghasem D. Najafpour ◽  
Ali Asghar Ghoreyshi
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Dry Weight ◽  
Optimal Growth ◽  
Inoculum Size ◽  
Effect Of Temperature ◽  
Lactic Acid Concentration ◽  
Initial Substrate

Abstract In batch fermentation of whey, selection of suitable species at desired conditions such as substrate, product concentrations, temperature and inoculum size were investigated. Four Lactobacillus species and one Lactococcus species were screened for lactic acid production. Among them L. bulgaricus ATCC 11842 were selected for further studies. The optimal growth of the selected organism for variable size of inocula was examined. The results indicated that inoculum size had insignificant effect on the cell and lactic acid concentration. The effect of temperature was also studied at 32, 37, 42 and 47°C. Results showed that the concentration of cell dry weight increased with increment of temperature from 32 to 42°C. The maximum cell and lactic acid concentration was obtained at 42°C. The effect of initial substrate concentration on lactic acid production was also examined. The optimum initial lactose concentration was found to be 90 g/l.

Optimization of Lactic Acid Production from Food Waste by the Saccharification of Bacillus subtili

2010 ◽  
Vol 113-116 ◽  
pp. 1080-1083 ◽  
Author(s):  
Ying Ying Liu ◽  
Qun Hui Wang ◽  
Li Wei Chen ◽  
Xiao Qiang Wang ◽  
Juan Wang
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Acid Concentration ◽  
Food Waste ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Total Sugar ◽  
Lactic Acid Concentration ◽  
Two Phase ◽  
Better Than

In order to reduce the costs of production and increase the lactic acid yields, this research adopts Bacillus subtilis to substitute enzymes. The method used in the study is two-phase fermentation - inoculate Bacillus subtilis to food waste to produce sugar, and then inoculate Lactobacillus to food waste to yield lactic acid. 87.22 g l–1 of total sugar can be obtained from non-autoclaved food waste in 30 h of saccharification at 40 centigrade. After two-phase fermentation, the optimal lactic acid concentration was 50.77g/L. The results indicate that two-phase fermentation is better than synchronous saccharification fermentation.

scholarly journals Utilization of Molasses Sugar for Lactic Acid Production by Lactobacillus delbrueckii subsp. delbrueckii Mutant Uc-3 in Batch Fermentation

2007 ◽  
Vol 74 (1) ◽  
pp. 333-335 ◽  
Author(s):  
Arti Dumbrepatil ◽  
Mukund Adsul ◽  
Shivani Chaudhari ◽  
Jayant Khire ◽  
Digambar Gokhale
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Acid Production ◽  
Batch Fermentation ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Potential Candidate ◽  
Lactic Acid Concentration ◽  
High Concentration ◽  
Acid Fermentation

ABSTRACT Efficient lactic acid production from cane sugar molasses by Lactobacillus delbrueckii mutant Uc-3 in batch fermentation process is demonstrated. Lactic acid fermentation using molasses was not significantly affected by yeast extract concentrations. The final lactic acid concentration increased with increases of molasses sugar concentrations up to 190 g/liter. The maximum lactic acid concentration of 166 g/liter was obtained at a molasses sugar concentration of 190 g/liter with a productivity of 4.15 g/liter/h. Such a high concentration of lactic acid with high productivity from molasses has not been reported previously, and hence mutant Uc-3 could be a potential candidate for economical production of lactic acid from molasses at a commercial scale.

scholarly journals Evaluation of conditions for fermentation of fish offal

1995 ◽  
Vol 4 (1) ◽  
pp. 11-17
Author(s):  
T. Mikael Lassén
Keyword(s):  
Lactic Acid ◽  
Redox Potential ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Storage Period ◽  
Growth Conditions ◽  
Inoculum Size ◽  
Reliable Estimate ◽  
Small Scale ◽  
Acid Fermentation

Conditions for the lactic acid fermentation of fish offal were evaluated regarding the effect of substrate concentration (2, 5 and 10% dextrose), preacidification with lactic acid (initial pH of 6.8, to 6.5 or 6.0), and inoculum size of Lactobacillus plantarum (107 , 108 and 109 colony forming units (cfu)/g). pH and lactic acid production were monitored during a two-week storage period. A small-scale silo for fermenting fish offal was also constructed, and measurement of redox potential was evaluated as a means to estimate bacterial growth conditions. The most favourable conditions for fermentation, manifested by a low and stable pH and high lactic acid production, were achieved with an inoculum size of 108 cfu/g and 5% dextrose. Preacidification did not affect final pH. Redox potential was shown to give a reliable estimate of growth conditions for bacteria under anaerobic conditions by rapidly falling to below -550mV in silage with a high lactic acid concentration.

Production of L-Lactic Acid from Corn Starch Hydrolysate by Immobilized Rhizopus oryzae on a New Asterisk-Shaped Matrix

2011 ◽  
Vol 347-353 ◽  
pp. 1193-1197 ◽  
Author(s):  
Xin Chen ◽  
Bing Bing Zhang ◽  
Yuan Liang Wang ◽  
Yan Feng Luo ◽  
Run Guang Wang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Concentration ◽  
Corn Starch ◽  
Rhizopus Oryzae ◽  
Immobilized Cells ◽  
Lactic Acid Production ◽  
Carbon Sources ◽  
Cotton Cloth ◽  
Lactic Acid Concentration ◽  
Starch Hydrolysate

A new asterisk-shaped matrix composed of stainless-steel mesh and cotton cloth was emloyed for immobilizing Rhizopus oryzae As3.3462 cells. The ability of the immobilized cells to produce L-lactic acid was evaluated by using corn starch hydrolysate as carbon sources. The cells were attached onto the matrix with nearly 100% efficiency within 5 h and demonstrated asterisk mycelia morphology rather than a pellet-like cake as exhibited by the free-cells. Consequently, the immobilization fermentation resulted in higher L-lactic acid concentration (82.79 g.L-1 vs 48.23 g.L-1) within a shorter time (60 h vs 72 h) than the free fermentation. In addition, the stability of the immobilized cells for a long-term fermentation was investigated in 8 consecutive fermentation batches for 30 days. The maximum variation of L-lactic acid concentration among these batches was less than 10%. These results imply that the proposed asterisk matrix is good for Rhizopus oryzae immobilization and provids a simple and feasible fermentation strategy for L-lactic acid production.

Screening of Lactic Acid Fermentation by Rhizopus oryzae on Banana Peel Using Two-Level Factorial Analysis

2022 ◽  
Vol 16 (1) ◽  
pp. 12
Author(s):  
Nor Atikah Husna Ahmad Nasir ◽  
Nurul Syafiqah Mohd Yaminudin ◽  
Atikah Kamaludin ◽  
Sharir Aizat Kamaruddin ◽  
Siti Nurbalqis Aziman
Keyword(s):  
Lactic Acid ◽  
Moisture Content ◽  
Acid Production ◽  
Rhizopus Oryzae ◽  
Lactic Acid Production ◽  
Inoculum Size ◽  
Factorial Analysis ◽  
Banana Peel ◽  
Acid Fermentation ◽  
Significant Difference

For ages, pure sugars or edible crops have produced lactic acid. However, a major concern on lactic acid production lies in the cost of the raw materials used. Thus, an alternative to overcome this situation is urgently needed. Characterization of banana peels shows that it contains promising sugar that can be utilized for lactic acid production, which are xylose (0.774 g/L), glucose (0.756 g/L) and fructose (0.532 g/L). Thus, this study aims to screen the potential of banana peel as a substrate by using Rhizopus oryzae through batch fermentation for lactic acid production, as R. oryzae can synthesize lactic acid in low nutrient requirements. Two-level factorial analysis was designed to screen the effects of moisture content (60% and 80%), temperature (27 °C and 40 °C), pH (4.5 and 6.5) and inoculum size (1x104 spores/mL and 1x108 spores/g) on the lactic acid production. Based on the Two-level factorial (2LF) analysis, the highest lactic acid production of 0.0813 g/L was detected at 80 % moisture content, pH 4.5, the temperature of 27 °C and inoculum size of 1x104 spores/g. The findings show that most of the conditions have a significant difference between each other (p<0.05). Therefore, the fermentation of banana peels by R. oryzae could be a promising method to produce a lactic acid concentration.

DETERMINATION OF LACTIC ACID PRODUCTION BY RHIZOPUS ORYZAE IN SOLID STATE FERMENTATION OF PINEAPPLE WASTE

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Siti Nurbalqis Aziman ◽  
Hasnaa’ Hazimah Tumari ◽  
Nor Azimah Mohd Zain
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Rhizopus Oryzae ◽  
Lactic Acid Production ◽  
Inoculum Size ◽  
Pineapple Waste ◽  
Good Agreement

Solid pineapple waste (SPW) is one of the most abundant agricultural wastes found in tropic region. This study is looking into the potential of utilising solid pineapple waste in solid state fermentation for the production of lactic acid by Rhizopus oryzae. A 2-level factorial design was employed to screen the effect of moisture content (60% and 80%), inoculum size (1×104 spores/g and 1×108 spores/g), pH (4.5 and 6.5), temperature (27°C and 40°C) and particle size (<0.5 mm and >3.15 mm) to the production of lactic acid. The predicted maximum production is 0.0221 g lactic acid/g SPW in SSF condition of 80% moisture; pH 6.5; 1×104 spores/g of inoculum; waste particle of 3.15 mm; and temperature 27°C. Analysis of variance (ANOVA) showed that the model is significant with high value of predicted (0.9616) and adjusted (0.9726) R-squared, indicated a good agreement between the predicted and actual values at each point of the experiment. Post-statistical experiment confirmed the ability of lactic acid production by R. oryzae at the predicted conditions with 0.0236 g lactic acid/g SPW being produced.

scholarly journals STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI

1956 ◽  
Vol 104 (1) ◽  
pp. 121-136 ◽  
Author(s):  
Hartmann Stähelin ◽  
Emanuel Suter ◽  
Manfred L. Karnovsky
Keyword(s):  
Oxygen Consumption ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Lactate Production ◽  
Dry Weight ◽  
Cell Types ◽  
Polymorphonuclear Leucocytes ◽  
Cell Counts ◽  
Mononuclear Leucocytes

As a basis for studies on the interaction of phagocytes and tubercle bacilli experiments were carried out to obtain information on some biochemical characteristics of exudate leucocytes from guinea pigs. It was found that total cellular phosphorus was the most suitable measure of protoplasm. Cell counts were less reliable because of unavoidable clumping in the suspension, and dry weight measurements were less specific when contamination with erythrocytes occurred. The utility of phosphorus measurements in this connection depends upon the fact that an erythrocyte contains only 4 per cent of the phosphorus present in a leucocyte. From measurements on mixed suspensions consisting of varying known proportions of polymorphonuclear and mononuclear leucocytes as determined by differential counting, it was possible to compute true values for these two cell types with respect to oxygen consumption and lactic acid production. Thus it was found that monocytes consumed considerably more oxygen and produced more lactate than polymorphonuclear leucocytes. From the data obtained it is suggested that differences in metabolic activity found when comparing cell suspensions obtained by the use of different irritants are due to different proportions of the two cell types. In particular, the effects of oxygen tension and pH on the activities of the cells were studied. It was found that decreasing the proportion of oxygen in the atmosphere from that of air to 1 per cent reduced oxygen consumption by about 80 per cent, whereas lactic acid production was increased by about 45 per cent. It was also found that decreasing the pH of the medium below pH 7.5 caused a considerable reduction in the respiration, lactate production, and viability of polymorphonuclear leucocytes. The monocytes proved less sensitive to similar changes in pH, especially with regard to lactic acid production and viability. Observations were made of oxygen consumption and lactate production during phagocytosis. During the hour following the addition of heat-killed tubercle bacilli to the phagocytes, the oxygen consumption of suspensions rich in polymorphonuclear leucocytes rose by 60 per cent, and that of suspensions rich in monocytes rose by nearly 100 per cent. Lactic acid production was unchanged during phagocytosis.

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