scholarly journals Physical cues of biomaterials guide stem cell fate of differentiation: The effect of elasticity of cell culture biomaterials

Open Physics ◽  
2018 ◽  
Vol 16 (1) ◽  
pp. 943-955 ◽  
Author(s):  
Vincent Irawan ◽  
Akon Higuchi ◽  
Toshiyuki Ikoma
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Cell Fate ◽  
Empirical Model ◽  
Three Dimensional ◽  
Distinct Cell Type ◽  
Distinct Cell ◽  
Direct Sensing ◽  
D Cell ◽  
Culture Environment

Abstract It is widely accepted that stem cells directly sense the elasticity of two-dimensional (2-D) substrates and differentiate into a distinct cell type dependent on the substrate elasticity (direct-sensing differentiation: soft and hard substrates promote differentiation into soft and hard tissue lineage, respectively). Biologically, native extracellular matrices (ECMs) are constantly remodeled through out the life of individuals, which inadvertently introduce changes of mechanical properties. Therefore, direct-sensing differentiation might not fully take into account the responses of stem cells in the actual ECMs microenvironment. Recent investigations in three-dimensional (3-D) cell culture environment suggested the inconsistency of direct-sensing differentiation. Stem cells specifically differentiate not only by sensing the elasticity of materials but also by considering the cellular traction exerted to reorganize the matrices and the matrices deformation. This paper aims to expand further how the cells incorporate the elasticity cues and traction-mediated deformation in final differentiation fates of stem cells. To achieve the aim, we introduced an empirical model based on the investigations in 3-D cell culture environment. The empirical model would serve as a useful framework for future studies intended to investigate the relationship of traction-mediated deformation and commitment of stem cells for variety of tissue lineage in 2-D or 3-D cell culture environment.

scholarly journals Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

2017 ◽  
Vol 37 (7) ◽  
pp. 647-660 ◽  
Author(s):  
Saradaprasan Muduli ◽  
Li-Hua Chen ◽  
Meng-Pei Li ◽  
Zhao-wen Heish ◽  
Cheng-Hui Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Fate ◽  
Es Cells ◽  
Embryonic Stem ◽  
Poly Vinyl Alcohol ◽  
Hematopoietic Stem ◽  
Stem Cell Culture

Abstract The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

scholarly journals Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From Two-Dimensional and Three-Dimensional Cell Culture Microenvironments

2019 ◽  
Author(s):  
Tiffany Miller
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
Three Dimensional ◽  
Inflammatory Biomarkers ◽  
Two Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell ◽  
Stimulation Of

<p>Bone marrow derived stem cells express biomarkers capable of facilitating adhesion to the cell culturing microenvironment, thereby, influencing their proliferation, migration, and differentiation. In particular, biological biomarkers of mesenchymal stem cells include, but are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+, CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between the stem cell biology and the materials and methods forming a cell culturing microenvironment serves as a critical aspect in the successful adhesion and growth within two-dimensional cell culture microenvironments such as polystyrene, laminin, fibronectin, or poly-L-lysine and within three-dimensional cell culture microenvironments such as hydrogel, ceramic, collagen, polymer based nanofibers, agitation, forced floating, and hang drop systems. Further, electrical stimulation of the stem cells may be implemented during the cell culturing process to measure stem cell growth and to determine stem cell viability. In addition, electrical stimulation of implanted stem cells may facilitate tracking by measuring stem cell migration distance and travel area. Although many biochemical and inflammatory biomarkers are expressed based on severity in stroke including, but not limited to, Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate (Glu), current methodologies of stem cell directing lack localization and biological effector specificity. Biological effector bound magnetic particle stem cells may serve as a potential treatment method in ischemic stroke. In particular, a stem cell biomarker may be configured to communicate with inflammatory biomarkers, thus, more efficiently delivering the stem cells to site specific areas having the most severely affected <i>in-vivo</i> biochemical microenvironments.</p>

scholarly journals Myf5 haploinsufficiency reveals distinct cell fate potentials for adult skeletal muscle stem cells

Development ◽  
2012 ◽  
Vol 139 (12) ◽  
pp. e1208-e1208
Author(s):  
B. Gayraud-Morel ◽  
F. Chretien ◽  
A. Jory ◽  
R. Sambasivan ◽  
E. Negroni ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Cell Fate ◽  
Adult Skeletal Muscle ◽  
Muscle Stem Cells ◽  
Distinct Cell ◽  
Skeletal Muscle Stem Cells

scholarly journals Growing, Tracking, and Directing Bone Marrow Derived Stem Cells From Two-Dimensional and Three-Dimensional Cell Culture Microenvironments

2019 ◽  
Author(s):  
Tiffany Miller
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Bone Marrow ◽  
Stem Cell ◽  
Three Dimensional ◽  
Inflammatory Biomarkers ◽  
Two Dimensional ◽  
Cell Culturing ◽  
Dimensional Cell ◽  
Stimulation Of

<p>Bone marrow derived stem cells express biomarkers capable of facilitating adhesion to the cell culturing microenvironment, thereby, influencing their proliferation, migration, and differentiation. In particular, biological biomarkers of mesenchymal stem cells include, but are not limited to, CD14-, CD19-, CD34-, CD45-, CD29, CD44, CD73+, CD90+, CD105+, CD106, CD166, Stro-1, and HLADR. The relationship between the stem cell biology and the materials and methods forming a cell culturing microenvironment serves as a critical aspect in the successful adhesion and growth within two-dimensional cell culture microenvironments such as polystyrene, laminin, fibronectin, or poly-L-lysine and within three-dimensional cell culture microenvironments such as hydrogel, ceramic, collagen, polymer based nanofibers, agitation, forced floating, and hang drop systems. Further, electrical stimulation of the stem cells may be implemented during the cell culturing process to measure stem cell growth and to determine stem cell viability. In addition, electrical stimulation of implanted stem cells may facilitate tracking by measuring stem cell migration distance and travel area. Although many biochemical and inflammatory biomarkers are expressed based on severity in stroke including, but not limited to, Interluken-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and glutamate (Glu), current methodologies of stem cell directing lack localization and biological effector specificity. Biological effector bound magnetic particle stem cells may serve as a potential treatment method in ischemic stroke. In particular, a stem cell biomarker may be configured to communicate with inflammatory biomarkers, thus, more efficiently delivering the stem cells to site specific areas having the most severely affected <i>in-vivo</i> biochemical microenvironments.</p>

scholarly journals Squaramide-Based Supramolecular Materials for Three-Dimensional Cell Culture of Human Induced Pluripotent Stem Cells and Their Derivatives

2018 ◽  
Vol 19 (4) ◽  
pp. 1091-1099 ◽  
Author(s):  
Ciqing Tong ◽  
Tingxian Liu ◽  
Victorio Saez Talens ◽  
Willem E. M. Noteborn ◽  
Thomas H. Sharp ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Three Dimensional ◽  
Supramolecular Materials ◽  
Induced Pluripotent ◽  
Dimensional Cell
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