Analytik modifizierter Steroidhormone mittels Flüssigkeitschromatographie/Tandem-Massenspektrometrie Analysis of modified steroid hormones using liquid chromatography/tandem mass spectrometry

2006 ◽  
Vol 30 (1) ◽  
pp. 26-32 ◽  
Author(s):  
Mario Thevis ◽  
Wilhelm Schänzer
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Steroid Hormones ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Determination of steroid hormones in sediments based on quick, easy, cheap, effective, rugged, and safe (modified-QuEChERS) extraction followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

2015 ◽  
Vol 7 (22) ◽  
pp. 9577-9586 ◽  
Author(s):  
Julien Camilleri ◽  
Emmanuelle Vulliet
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Steroid Hormones ◽  
Tandem Mass ◽  
Modified Quechers ◽  
Chromatography Tandem Mass Spectrometry ◽  
Target Analytes ◽  
Liquid Chromatography Tandem Mass

The method enables the determination of the target analytes with limits of detection comprised between 0.03 and 0.2 ng g¬1and limits of quantification comprised between 0.1 and 0.6 ng g¬1.

scholarly journals Liquid chromatography tandem mass spectrometry for the quantification of steroid hormone profiles in blubber from stranded humpback whales (Megaptera novaeangliae)

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Greta Dalle Luche ◽  
Susan Bengtson Nash ◽  
John R Kucklick ◽  
Fletcher M J Mingramm ◽  
Ashley S P Boggs
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Steroid Hormones ◽  
Megaptera Novaeangliae ◽  
Humpback Whales ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Relative Standard ◽  
Liquid Chromatography Tandem Mass

Abstract Marine mammal blubber is known to have quantifiable concentrations of steroid hormones and is increasingly chosen as a matrix for the detection of these reproductive and stress biomarkers. Steroid hormones act through complex cascades, often in concert, yet studies conducted on cetaceans have rarely measured more than two steroids simultaneously. Due to the role of steroid hormones in multiple physiological processes, and variability in concentration among individuals, data on single compounds are often difficult to interpret. Here a liquid chromatography tandem mass spectrometry method for the simultaneous analyses of multiple steroid hormones in cetacean blubber was validated and applied to samples from 10 stranded humpback whales (Megaptera novaeangliae). Progesterone, 17α-hydroxyprogesterone, testosterone, androstenedione, oestrone, oestradiol, cortisone, cortisol, corticosterone and 11-deoxycorticosterone were reliably (relative standard deviation on six replicates <15%) and accurately (recovery of an amended sample between 70% and 120%) quantified, but not 11-deoxycortisol. With the exception of progesterone, testosterone, oestradiol and cortisol, these compounds were quantified for the first time in humpback whales. Given that blubber is frequently collected from free-swimming cetaceans in ongoing research programs, the technique developed here could substantially strengthen understanding and monitoring of the physiological condition of these species.

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