Fish Embryo Test (FET)

2017 ◽  
Author(s):  
John H. Duffus ◽  
Michael Schwenk ◽  
Douglas M. Templeton
Keyword(s):  
Fish Embryo ◽  
Fish Embryo Test

The fish embryo test (FET): origin, applications, and future

2014 ◽  
Vol 22 (21) ◽  
pp. 16247-16261 ◽  
Author(s):  
Thomas Braunbeck ◽  
Britta Kais ◽  
Eva Lammer ◽  
Jens Otte ◽  
Katharina Schneider ◽  
...  
Keyword(s):  
Fish Embryo ◽  
Fish Embryo Test

DMSO modifies the permeability of the zebrafish (Danio rerio) chorion-Implications for the fish embryo test (FET)

2013 ◽  
Vol 140-141 ◽  
pp. 229-238 ◽  
Author(s):  
B. Kais ◽  
K.E. Schneider ◽  
S. Keiter ◽  
K. Henn ◽  
C. Ackermann ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Fish Embryo ◽  
Fish Embryo Test

PAH toxicity at aqueous solubility in the fish embryo test with Danio rerio using passive dosing

Chemosphere ◽  
2014 ◽  
Vol 112 ◽  
pp. 77-84 ◽  
Author(s):  
Thomas-Benjamin Seiler ◽  
Nina Best ◽  
Margit Møller Fernqvist ◽  
Hendrik Hercht ◽  
Kilian E.C. Smith ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Aqueous Solubility ◽  
Fish Embryo ◽  
Fish Embryo Test ◽  
Passive Dosing ◽  
Pah Toxicity

The fish embryo test as an alternative to acute fish toxicity testing: Optimisation for difficult compounds and role of metabolic activation

2009 ◽  
Vol 189 ◽  
pp. S205
Author(s):  
Stefan Scholz ◽  
Melanie Knöbel ◽  
Julia Ortmann ◽  
Frans Busser ◽  
Nynke Kramer ◽  
...  
Keyword(s):  
Toxicity Testing ◽  
Metabolic Activation ◽  
Fish Embryo ◽  
Fish Embryo Test ◽  
Fish Toxicity

scholarly journals Brazilian silverside, Atherinella brasiliensis (Quoy & Gaimard,1825) embryos as a test-species for marine fish ecotoxicological tests

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11214
Author(s):  
Natália Martins Feitosa ◽  
Emiliano Nicolas Calderon ◽  
Rhennã Nascimento da Silva ◽  
Sônia Lopes Rezende de Melo ◽  
Jackson Souza-Menezes ◽  
...  
Keyword(s):  
Marine Fish ◽  
Alternative Assessment ◽  
Assessment Tool ◽  
South American Species ◽  
Fish Embryo ◽  
Test Species ◽  
Ecotoxicological Tests ◽  
Fish Embryo Test ◽  
Natural Reproduction ◽  
Contaminated Waste

The fish embryo test (FET) is an alternative to the classic freshwater toxicity test used to assess environmental hazards and risks to fish. This test has been standardized and adopted by the Organization for Economic and Cooperation and Development (OECD). As salinity may affect the substances’ toxicity, we describe the development of an alternative euryhaline test species for embryonic ecotoxicological tests: the Brazilian silverside Atherinella brasiliensis (Quoy & Gaimard, 1825). This species is broadly distributed along the coast of South America and is able to inhabit a broad range of environmental and saline conditions. Ours is the first study on the maintenance of a native South American species for natural reproduction and the generation of embryos for tests. The embryos used are transparent and possess fluorescent cells which have only been seen in a few species and which may be used as markers, making it an alternative assessment tool for the lethal and sublethal substances in marine and estuarine environments. We provide a detailed description and analysis of embryonic development under different salinities and temperatures. The embryos and larvae developed in similar ways at different salinities, however as temperatures increased, mortality also increased. We considered the effects of the reference toxicants Zn2+ and SDS using a protocol similar to the FET that was standardized for zebrafish. Brazilian silverside embryos are as sensitive as freshwater, or euryhaline fish, to the surfactant but are more resistant to metals prior to hatching. We were able to show the advantages of the Brazilian silverside as a model for a marine fish embryo test (FETm) with high levels of reproducibility and little contaminated waste.

scholarly journals Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

2019 ◽  
Vol 7 ◽  
Author(s):  
Bettie Cormier ◽  
Annika Batel ◽  
Jérôme Cachot ◽  
Marie-Laure Bégout ◽  
Thomas Braunbeck ◽  
...  
Keyword(s):  
Hazard Assessment ◽  
Danio Rerio ◽  
Fish Embryo ◽  
Fish Embryo Test

Is fish embryo test (FET) according to OECD 236 sensible enough for delivering quality data for effluent risk assessment?

2018 ◽  
Vol 37 (11) ◽  
pp. 2925-2932 ◽  
Author(s):  
Julio Alberto Alegre Stelzer ◽  
Catiusa Kuchak Rosin ◽  
Luana Hainzenreder Bauer ◽  
Marilia Hartmann ◽  
Fernando Hepp Pulgati ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Quality Data ◽  
Fish Embryo ◽  
Fish Embryo Test

Species-specific considerations in using the fish embryo test as an alternative to identify endocrine disruption

2014 ◽  
Vol 155 ◽  
pp. 62-72 ◽  
Author(s):  
Viktoria Schiller ◽  
Xiaowei Zhang ◽  
Markus Hecker ◽  
Christoph Schäfers ◽  
Rainer Fischer ◽  
...  
Keyword(s):  
Endocrine Disruption ◽  
Fish Embryo ◽  
Fish Embryo Test ◽  
Species Specific

scholarly journals Toxic or Not Toxic, That Is the Carbon Quantum Dot’s Question: A Comprehensive Evaluation with Zebrafish Embryo, Eleutheroembryo, and Adult Models

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1598
Author(s):  
Chih-Yu Chung ◽  
Yu-Ju Chen ◽  
Chia-Hui Kang ◽  
Hung-Yun Lin ◽  
Chih-Ching Huang ◽  
...  
Keyword(s):  
Zebrafish Embryo ◽  
Comprehensive Evaluation ◽  
Aquatic Organisms ◽  
Carbon Quantum Dots ◽  
Adult Zebrafish ◽  
Fish Embryo ◽  
Wide Range ◽  
Different Types ◽  
Low Toxicity

Carbon quantum dots (CQDs) are emerging novel nanomaterials with a wide range of applications and high biocompatibility. However, there is a lack of in-depth research on whether CQDs can cause acute or long-term adverse reactions in aquatic organisms. In this study, two different types of CQDs prepared by ammonia citrate and spermidine, namely CQDAC and CQDSpd, were used to evaluate their biocompatibilities. In the fish embryo acute toxicity test (FET), the LD50 of CQDAC and CQDSpd was about 500 and 100 ppm. During the stage of eleutheroembryo, the LD50 decreased to 340 and 55 ppm, respectively. However, both CQDs were quickly eliminated from embryo and eleutheroembryo, indicating a lack of bioaccumulation. Long-term accumulation of CQDs was also performed in this study, and adult zebrafish showed no adverse effects in 12 weeks. In addition, there was no difference in the hatchability and deformity rates of offspring produced by adult zebrafish, regardless of whether they were fed CQDs or not. The results showed that both CQDAC and CQDSpd have low toxicity and bioaccumulation to zebrafish. Moreover, the toxicity assay developed in this study provides a comprehensive platform to assess the impacts of CQDs on aquatic organisms in the future.

scholarly journals The Role of Polymeric Coatings for a Safe-by-Design Development of Biomedical Gold Nanoparticles Assessed in Zebrafish Embryo

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1004
Author(s):  
Pamela Floris ◽  
Stefania Garbujo ◽  
Gabriele Rolla ◽  
Marco Giustra ◽  
Lucia Salvioni ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Significant Inhibition ◽  
Zebrafish Embryos ◽  
Design Development ◽  
Embryo Viability ◽  
Fish Embryo ◽  
Toxic Response ◽  
Consequent Reduction ◽  
Vertebrate Model ◽  
Safe By Design

In the biomedical field, gold nanoparticles (GNPs) have attracted the attention of the scientific community thanks to their high potential in both diagnostic and therapeutic applications. The extensive use of GNPs led researchers to investigate their toxicity, identifying stability, size, shape, and surface charge as key properties determining their impact on biological systems, with possible strategies defined to reduce it according to a Safe-by-Design (SbD) approach. The purpose of the present work was to analyze the toxicity of GNPs of various sizes and with different coating polymers on the developing vertebrate model, zebrafish. In particular, increasing concentrations (from 0.001 to 1 nM) of 6 or 15 nm poly-(isobutylene-alt-maleic anhydride)-graft-dodecyl polymer (PMA)- or polyethylene glycol (PEG)-coated GNPs were tested on zebrafish embryos using the fish embryo test (FET). While GNP@PMA did not exert significant toxicity on zebrafish embryos, GNP@PEG induced a significant inhibition of embryo viability, a delay of hatching (with the smaller size NPs), and a higher incidence of malformations, in terms of tail morphology and eye development. Transmission electron microscope analysis evidenced that the more negatively charged GNP@PMA was sequestered by the positive charges of chorion proteins, with a consequent reduction in the amount of NPs able to reach the developing embryo and exert toxicological activity. The mild toxic response observed on embryos directly exposed to GNP@PMA suggest that these NPs are promising in terms of SbD development of gold-based biomedical nanodevices. On the other hand, the almost neutral GNP@PEG, which did not interact with the chorion surface and was free to cross chorion pores, significantly impacted the developing zebrafish. The present study raises concerns about the safety of PEGylated gold nanoparticles and contributes to the debated issue of the free use of this nanotool in medicine and nano-biotechnologies.

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