Hypochromic Effect

2016 ◽  
Author(s):  
S. E. Braslavsky ◽  
K. N. Houk
Keyword(s):  
Hypochromic Effect

scholarly journals The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Olga Ilinskaya ◽  
Vera Ulyanova ◽  
Irina Lisevich ◽  
Elena Dudkina ◽  
Nataliya Zakharchenko ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Protein Concentration ◽  
Bacillus Pumilus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Culture Fluid ◽  
Size Exclusion ◽  
High Protein Concentration ◽  
Exclusion Chromatography ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Hypochromic Effect

Supported by crystallography studies, secreted ribonuclease of Bacillus pumilus (binase) has long been considered to be monomeric in form. Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric. To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase. The immutability of binase secondary structure upon transition from low to high protein concentration was registered, suggesting the binase dimerization immediately after translocation through the cell membrane and leading to detection of binase dimers only in the culture fluid regardless of ribonuclease concentration. Our results made it necessary to take a fresh look at the binase stability and cytotoxicity towards virus-infected or tumor cells.

scholarly journals SPECTROSCOPIC STUDY OF THE PROCESS OF HYPOCHROMIC EFFECT IN SOL T IN SOLUTIONS OF N UTIONS OF NATURAL D TURAL DYES AND VI YES AND VITAMIN B2 AMIN B2

2020 ◽  
Vol 4 (3) ◽  
pp. 21-27
Author(s):  
Salikh Khusenovich Astanov ◽  
◽  
Siddiq Kakhkharovich Kakhkharov ◽  
Keyword(s):  
Spectroscopic Study ◽  
Binary Mixtures ◽  
Self Assembly ◽  
Spectroscopic Method ◽  
Dipole Interaction ◽  
Absorption Capacity ◽  
Vitamin B2 ◽  
Intense Absorption ◽  
Hypochromic Effect

The process of self-assembly of riboflavin molecules in aqueous and binary mixtures of solvents was investigated by the spectroscopic method. It was shown that during the assembly of vitamin B2 molecules, dipole-dipole interaction occurs, as a result of which resonant splitting of excited electronic levels of riboflavin molecules occurs. And the observable hypo chromic effect is due to a decrease in the intense absorption capacity of the aggregated riboflavin molecules themselves.

Photocatalytic Degradation of Humic Acid Using Ti/Anodized TiO2 Metal Plate with Fe-Doping

2012 ◽  
Vol 724 ◽  
pp. 33-36 ◽  
Author(s):  
Jong Oh Kim ◽  
Seung Pil Choi ◽  
Geon Tae Kim ◽  
Yong Hak Kim
Keyword(s):  
Humic Acid ◽  
Photocatalytic Degradation ◽  
Surface Area ◽  
Acid Concentration ◽  
Uv Irradiation ◽  
Metal Plate ◽  
Fe Doping ◽  
Treatment Efficiency ◽  
Acid Degradation ◽  
Hypochromic Effect

We evaluated the treatment efficiency of humic acid using Ti and anodized TiO2metal plate with/without Fe-doping. Variation of humic acid concentration after 60 minutes of UV irradiation in the case of Ti only, Fe-Ti, TiO2only and Fe-TiO2was about 3.0%, 5.5%, 9.8% and 9.2%, respectively. . It is found that hypochromic effect was revealed in all cases with respect to doping time. Fe doping method is considered to be effective for humic acid degradation in spite of relatively low surface area of Ti and anodized TiO2metal plate.

Hypochromic Effect

2016 ◽  
Author(s):  
S. E. Braslavsky
Keyword(s):  
Hypochromic Effect

Coumarin–nucleotide base pairs. Ultraviolet absorption, fluorescence, and photochemical study

1984 ◽  
Vol 62 (10) ◽  
pp. 2006-2010 ◽  
Author(s):  
Grażyna Wenska ◽  
Stefan Paszyc
Keyword(s):  
Excitation Energy ◽  
Fluorescence Intensity ◽  
Ultraviolet Absorption ◽  
Base Pairs ◽  
Nucleotide Base ◽  
Polymethylene Chain ◽  
Hypochromic Effect

Bichromophoric compounds containing a nucleotide base and coumarin linked with a polymethylene chain have been synthesized. A hypochromic effect was observed for adenine–coumarin pairs in contrast to the thymine containing compound. The fluorescence intensity of coumarin when bound into these systems was found to be significantly reduced. It has also been demonstrated that excitation energy is transferred from adenine or thymine to the coumarin moiety. Intermolecular dimerization of coumarin residues was observed upon irradiation of an adenine–coumarin pair.

scholarly journals Interaction of Duloxetine Hydrochloride with Deoxyribonucleic Acid Measured by Fluorescence Spectroscopy

2016 ◽  
Vol 14 (2) ◽  
pp. 199-206
Author(s):  
Raznin Akter Joly ◽  
Md Reazul Islam ◽  
Sonia Sultana ◽  
Asma Rahman ◽  
Md Zakir Sultan ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Deoxyribonucleic Acid ◽  
Antidepressant Drug ◽  
Living Organism ◽  
Binding Mode ◽  
Duloxetine Hydrochloride ◽  
Calf Thymus ◽  
Binding Forces ◽  
Ct Dna ◽  
Hypochromic Effect

Interactions with many clinically active therapeutic agents with DNA are well studied and it is necessary to decipher the structure of DNA and to investigate the pathological implications of those molecules in living organism. This study investigated the interaction of antidepressant drug Duloxetine-hydrochloride (DLX) with calf thymus DNA (ct-DNA). The interaction of DLX with ct-DNA was studied employing fluorescence spectroscopy. Hypochromic effect was found in the absorption spectra of duloxetine, and its wavelength had no shift in the presence of DNA indicating external binding mode of duloxetine to DNA. Fluorescence spectroscopic results showed the quenching of fluorescence intensity of DLX in presence of DNA indicating the interaction between DLX and DNA. Hydrophobic interaction and hydrogen bonding played the dominating role in DLX-DNA binding and binding forces also indicate the binding site of duloxetine to be at the minor groove of DNA.Dhaka Univ. J. Pharm. Sci. 14(2): 199-206, 2015 (December)

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